Restriction map for
pFN21AB7372
(Linear) (Six-base) MAPSORT of: KIBB7372.seq Check: 2162 from: 1 to: 885
KIBB7372.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AatII G_ACGT'C
Cuts at: 0 614 885
Size: 614 271
AccI GT'mk_AC
Cuts at: 0 740 885
Size: 740 145
AflIII A'CryG_T
Cuts at: 0 638 715 885
Size: 638 77 170
Fragments arranged by size:
638 170 77
BanI G'GyrC_C
Cuts at: 0 128 885
Size: 128 757
BbsI GAAGACnn'nnnn_
Cuts at: 0 657 885
Size: 657 228
BfrBI ATG'CAT
Cuts at: 0 24 54 885
Size: 24 30 831
Fragments arranged by size:
831 30 24
BglII A'GATC_T
Cuts at: 0 599 885
Size: 599 286
BmgBI CAC'GTC
Cuts at: 0 783 885
Size: 783 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 885
Size: 730 155
BsaAI yAC'GTr
Cuts at: 0 641 885
Size: 641 244
BsaBI GATnn'nnATC
Cuts at: 0 226 885
Size: 226 659
BsaHI Gr'CG_yC
Cuts at: 0 611 885
Size: 611 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 885
Size: 121 30 734
Fragments arranged by size:
734 121 30
BseYI C'CCAG_C
Cuts at: 0 98 885
Size: 98 787
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 184 524 885
Size: 133 51 340 361
Fragments arranged by size:
361 340 133 51
BsiHKAI G_wGCw'C
Cuts at: 0 14 885
Size: 14 871
Bsp1286I G_dGCh'C
Cuts at: 0 14 885
Size: 14 871
BssSI C'ACGA_G
Cuts at: 0 670 885
Size: 670 215
BstEII G'GTnAC_C
Cuts at: 0 150 885
Size: 150 735
BstYI r'GATC_y
Cuts at: 0 227 599 885
Size: 227 372 286
Fragments arranged by size:
372 286 227
BtgI C'CryG_G
Cuts at: 0 36 428 684 885
Size: 36 392 256 201
Fragments arranged by size:
392 256 201 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 63 885
Size: 63 822
BtsI GCAGTG_nn'
Cuts at: 0 121 885
Size: 121 764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 18 53 71 106 336 371 885
Size: 18 35 18 35 230 35 514
Fragments arranged by size:
514 230 35 35 35 18 18
EaeI y'GGCC_r
Cuts at: 0 39 885
Size: 39 846
EarI CTCTTCn'nnn_
Cuts at: 0 457 614 885
Size: 457 157 271
Fragments arranged by size:
457 271 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 771 885
Size: 771 114
MscI TGG'CCA
Cuts at: 0 41 885
Size: 41 844
MspA1I CmG'CkG
Cuts at: 0 102 885
Size: 102 783
NcoI C'CATG_G
Cuts at: 0 428 885
Size: 428 457
NsiI A_TGCA'T
Cuts at: 0 26 56 885
Size: 26 30 829
Fragments arranged by size:
829 30 26
NspI r_CATG'y
Cuts at: 0 24 28 58 552 885
Size: 24 4 30 494 333
Fragments arranged by size:
494 333 30 24 4
PasI CC'CwG_GG
Cuts at: 0 767 885
Size: 767 118
PsiI TTA'TAA
Cuts at: 0 311 885
Size: 311 574
PvuII CAG'CTG
Cuts at: 0 102 885
Size: 102 783
SfcI C'TryA_G
Cuts at: 0 534 885
Size: 534 351
SmlI C'TyrA_G
Cuts at: 0 745 885
Size: 745 140
SphI G_CATG'C
Cuts at: 0 24 28 58 552 885
Size: 24 4 30 494 333
Fragments arranged by size:
494 333 30 24 4
StuI AGG'CCT
Cuts at: 0 777 885
Size: 777 108
StyI C'CwwG_G
Cuts at: 0 86 298 428 855 885
Size: 86 212 130 427 30
Fragments arranged by size:
427 212 130 86 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 885
Size: 146 739
TatI w'GTAC_w
Cuts at: 0 285 885
Size: 285 600
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 885
Size: 121 32 732
Fragments arranged by size:
732 121 32
ZraI GAC'GTC
Cuts at: 0 612 885
Size: 612 273
Enzymes that do cut:
AatII AccI AflIII BanI BbsI BfrBI BglII BmgBI
BpuEI BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BssSI BstEII BstYI BtgI BtgZI BtsI CspCI
EaeI EarI EcoNI MscI MspA1I NcoI NsiI NspI
PasI PsiI PvuII SfcI SmlI SphI StuI StyI
TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BsaI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI