(Circular) (Six-base) MAPSORT of: pFN21AB7366.seq Check: 2 from: 1 to: 5440 pFN21AB7366 5440 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2297 2465 2297 Size: 168 5272 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3503 4887 5155 1632 Size: 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 268 AfeI AGC'GCT Cuts at: 2426 2426 Size: 5440 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 2035 2181 2224 3183 4755 2035 Size: 146 43 959 1572 2720 Fragments arranged by size: 2720 1572 959 146 43 ApaI G_GGCC'C Cuts at: 2349 2524 2716 2349 Size: 175 192 5073 Fragments arranged by size: 5073 192 175 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 2502 3994 160 Size: 872 1470 1492 1606 Fragments arranged by size: 1606 1492 1470 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2765 1870 Size: 73 207 615 4545 Fragments arranged by size: 4545 615 207 73 BamHI G'GATC_C Cuts at: 2352 2770 3137 2352 Size: 418 367 4655 Fragments arranged by size: 4655 418 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 4216 618 Size: 325 217 627 974 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 BanII G_rGCy'C Cuts at: 1391 2349 2524 2716 2759 1391 Size: 958 175 192 43 4072 Fragments arranged by size: 4072 958 192 175 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4051 136 Size: 107 122 71 631 605 2379 1525 Fragments arranged by size: 2379 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2071 2349 2403 2524 2716 3501 4657 1790 Size: 281 278 54 121 192 785 1156 2573 Fragments arranged by size: 2573 1156 785 281 278 192 121 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2269 3171 4129 Size: 416 53 83 399 24 852 902 958 Cuts at: 4129 442 Size: 1753 Fragments arranged by size: 1753 958 902 852 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2541 2602 4100 1239 Size: 1302 61 1498 2579 Fragments arranged by size: 2579 1498 1302 61 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3565 4430 4728 3565 Size: 865 298 4277 Fragments arranged by size: 4277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5215 Fragments arranged by size: 5215 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2687 1186 Size: 1501 3939 BseYI C'CCAG_C Cuts at: 2070 2144 2708 4643 5139 2070 Size: 74 564 1935 496 2371 Fragments arranged by size: 2371 1935 564 496 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 2194 2210 1163 Size: 573 449 9 16 4393 Fragments arranged by size: 4393 573 449 16 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2517 2626 2890 3651 4679 1540 Size: 275 186 516 109 264 761 1028 2301 Fragments arranged by size: 2301 1028 761 516 275 264 186 109 BsiHKAI G_wGCw'C Cuts at: 2333 3501 3586 4657 2333 Size: 1168 85 1071 3116 Fragments arranged by size: 3116 1168 1071 85 BsmI GAATG_Cn' Cuts at: 2197 2388 2710 2951 3044 2197 Size: 191 322 241 93 4593 Fragments arranged by size: 4593 322 241 191 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2724 2724 Size: 5440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2333 2349 2403 2524 2716 2759 Size: 399 281 262 16 54 121 192 43 Cuts at: 2759 3501 3586 4657 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 281 262 192 121 85 54 43 16 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2297 2350 2465 2795 844 Size: 1265 188 53 115 330 3489 Fragments arranged by size: 3489 1265 330 188 115 53 BsrBI CCG'CTC Cuts at: 1394 1823 2622 3327 1394 Size: 429 799 705 3507 Fragments arranged by size: 3507 799 705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4109 65 Size: 1183 2687 174 1396 Fragments arranged by size: 2687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 2247 96 Size: 2151 3289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 2721 3500 4512 2721 Size: 779 1012 3649 Fragments arranged by size: 3649 1012 779 BstAPI GCAn_nnn'nTGC Cuts at: 794 2026 2035 794 Size: 1232 9 4199 Fragments arranged by size: 4199 1232 9 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2352 2770 3137 3522 3539 4991 5077 5089 Size: 380 418 367 385 17 1452 86 12 Cuts at: 5089 5435 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 418 385 380 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2494 2494 Size: 5440 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2205 2514 Size: 54 1425 50 275 18 337 38 309 Cuts at: 2514 2623 2679 2697 2887 3776 5405 8 Size: 109 56 18 190 889 1629 43 Fragments arranged by size: 1629 1425 889 337 309 275 190 109 56 54 50 43 38 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2514 2623 2887 1537 Size: 275 702 109 264 4090 Fragments arranged by size: 4090 702 275 264 109 EarI CTCTTCn'nnn_ Cuts at: 1353 3370 1353 Size: 2017 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2541 2602 3503 4100 4887 5155 1239 Size: 393 909 61 901 597 787 268 1524 Fragments arranged by size: 1524 909 901 787 597 393 268 61 EcoO109I rG'GnC_Cy Cuts at: 2345 2346 2520 2521 2712 2713 2345 Size: 1 174 1 191 1 5072 Fragments arranged by size: 5072 191 174 1 1 1 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 5440 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 2299 2428 4587 1678 Size: 621 129 2159 2531 Fragments arranged by size: 2531 2159 621 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373 Size: 32 135 32 2509 32 42 32 2626 Fragments arranged by size: 2626 2509 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2365 2784 3030 669 Size: 988 281 427 419 246 3079 Fragments arranged by size: 3079 988 427 419 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 2681 10 Size: 54 1425 343 337 512 2769 Fragments arranged by size: 2769 1425 512 343 337 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2636 3398 3757 3916 518 Size: 648 843 627 762 359 159 2042 Fragments arranged by size: 2042 843 762 648 627 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2316 2628 2856 3533 4681 4926 1606 Size: 426 284 312 228 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 426 312 284 245 228 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2623 2887 2623 Size: 264 5176 NspI r_CATG'y Cuts at: 1132 1229 2798 4343 1132 Size: 97 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 97 PasI CC'CwG_GG Cuts at: 1348 2656 1348 Size: 1308 4132 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PfoI T'CCnGG_A Cuts at: 2307 2307 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2316 1613 Size: 703 4737 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2345 2520 2712 2345 Size: 175 192 5073 Fragments arranged by size: 5073 192 175 PstI C_TGCA'G Cuts at: 830 2531 2792 830 Size: 1701 261 3478 Fragments arranged by size: 3478 1701 261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2032 2316 2032 Size: 284 5156 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2527 2608 2788 3923 4604 4795 826 Size: 219 1482 81 180 1135 681 191 1471 Fragments arranged by size: 1482 1471 1135 681 219 191 180 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3544 4445 4707 820 Size: 197 926 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 2577 1517 Size: 1060 4380 StyI C'CwwG_G Cuts at: 513 1065 2004 2879 513 Size: 552 939 875 3074 Fragments arranged by size: 3074 939 875 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2350 3467 3484 3637 3822 4267 325 Size: 1255 770 1117 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1117 770 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 3686 5398 Size: 274 80 33 51 1443 270 1439 1712 Cuts at: 5398 96 Size: 138 Fragments arranged by size: 1712 1443 1439 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5400 5432 5400 Size: 32 5408 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2674 2674 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI