Restriction map for
pFN21AB7366
(Linear) (Six-base) MAPSORT of: KIBB7366.seq Check: 4936 from: 1 to: 735
KIBB7366.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 292 460 735
Size: 292 168 275
Fragments arranged by size:
292 275 168
AfeI AGC'GCT
Cuts at: 0 421 735
Size: 421 314
AlwNI CAG_nnn'CTG
Cuts at: 0 30 176 219 735
Size: 30 146 43 516
Fragments arranged by size:
516 146 43 30
ApaI G_GGCC'C
Cuts at: 0 344 519 711 735
Size: 344 175 192 24
Fragments arranged by size:
344 192 175 24
AseI AT'TA_AT
Cuts at: 0 497 735
Size: 497 238
AvaI C'yCGr_G
Cuts at: 0 145 735
Size: 145 590
BamHI G'GATC_C
Cuts at: 0 347 735
Size: 347 388
BanII G_rGCy'C
Cuts at: 0 344 519 711 735
Size: 344 175 192 24
Fragments arranged by size:
344 192 175 24
Bme1580I G_kGCm'C
Cuts at: 0 66 344 398 519 711 735
Size: 66 278 54 121 192 24
Fragments arranged by size:
278 192 121 66 54 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 735
Size: 264 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 597 735
Size: 536 61 138
Fragments arranged by size:
536 138 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 682 735
Size: 682 53
BseYI C'CCAG_C
Cuts at: 0 65 139 703 735
Size: 65 74 564 32
Fragments arranged by size:
564 74 65 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 189 205 735
Size: 180 9 16 530
Fragments arranged by size:
530 180 16 9
BsiEI CG_ry'CG
Cuts at: 0 512 621 735
Size: 512 109 114
Fragments arranged by size:
512 114 109
BsiHKAI G_wGCw'C
Cuts at: 0 328 735
Size: 328 407
BsmI GAATG_Cn'
Cuts at: 0 192 383 705 735
Size: 192 191 322 30
Fragments arranged by size:
322 192 191 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 719 735
Size: 719 16
Bsp1286I G_dGCh'C
Cuts at: 0 66 328 344 398 519 711 735
Size: 66 262 16 54 121 192 24
Fragments arranged by size:
262 192 121 66 54 24 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 292 345 460 735
Size: 104 188 53 115 275
Fragments arranged by size:
275 188 115 104 53
BsrBI CCG'CTC
Cuts at: 0 617 735
Size: 617 118
BsrGI T'GTAC_A
Cuts at: 0 242 735
Size: 242 493
BssSI C'ACGA_G
Cuts at: 0 716 735
Size: 716 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 21 30 735
Size: 21 9 705
Fragments arranged by size:
705 21 9
BstYI r'GATC_y
Cuts at: 0 347 735
Size: 347 388
Bsu36I CC'TnA_GG
Cuts at: 0 489 735
Size: 489 246
EaeI y'GGCC_r
Cuts at: 0 162 200 509 618 674 692 735
Size: 162 38 309 109 56 18 43
Fragments arranged by size:
309 162 109 56 43 38 18
EagI C'GGCC_G
Cuts at: 0 509 618 735
Size: 509 109 117
Fragments arranged by size:
509 117 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 597 735
Size: 536 61 138
Fragments arranged by size:
536 138 61
EcoO109I rG'GnC_Cy
Cuts at: 0 340 341 515 516 707 708 735
Size: 340 1 174 1 191 1 27
Fragments arranged by size:
340 191 174 27 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 312 735
Size: 312 423
HaeII r_GCGC'y
Cuts at: 0 294 423 735
Size: 294 129 312
Fragments arranged by size:
312 294 129
HincII GTy'rAC
Cuts at: 0 360 735
Size: 360 375
MscI TGG'CCA
Cuts at: 0 164 676 735
Size: 164 512 59
Fragments arranged by size:
512 164 59
MslI CAynn'nnrTG
Cuts at: 0 631 735
Size: 631 104
MspA1I CmG'CkG
Cuts at: 0 27 311 623 735
Size: 27 284 312 112
Fragments arranged by size:
312 284 112 27
NotI GC'GGCC_GC
Cuts at: 0 618 735
Size: 618 117
PasI CC'CwG_GG
Cuts at: 0 651 735
Size: 651 84
PfoI T'CCnGG_A
Cuts at: 0 302 735
Size: 302 433
PshAI GACnn'nnGTC
Cuts at: 0 311 735
Size: 311 424
PspOMI G'GGCC_C
Cuts at: 0 340 515 707 735
Size: 340 175 192 28
Fragments arranged by size:
340 192 175 28
PstI C_TGCA'G
Cuts at: 0 526 735
Size: 526 209
PvuII CAG'CTG
Cuts at: 0 27 311 735
Size: 27 284 424
Fragments arranged by size:
424 284 27
SfcI C'TryA_G
Cuts at: 0 522 603 735
Size: 522 81 132
Fragments arranged by size:
522 132 81
StuI AGG'CCT
Cuts at: 0 572 735
Size: 572 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 345 735
Size: 345 390
TatI w'GTAC_w
Cuts at: 0 242 735
Size: 242 493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 735
Size: 669 66
Enzymes that do cut:
AarI AfeI AlwNI ApaI AseI AvaI BamHI BanII
Bme1580I BmrI BpmI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI
BstYI Bsu36I EaeI EagI Eco57MI EcoO109I FalI HaeII
HincII MscI MslI MspA1I NotI PasI PfoI PshAI
PspOMI PstI PvuII SfcI StuI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AsiSI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BspEI BspHI BsrDI BsrFI BssHII BstBI BstEII
BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI