Restriction map for
pFN21AB7365
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BseRI BsgI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 463 TCCGGA T^CCGGA
AcyI 13 GACGCC G[GA]^CG[CT]C
AfaI 437 GTAC GT^AC
AlwI 60 GATCC GGATC(4/5)
Asp700I 359 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 41 GTGCTC G[AT]GC[AT]^C
AvrII 31 CCTAGG C^CTAGG
BbsI 553 GAAGAC GAAGAC(2/6)
BbvI 516 GCAGC GCAGC(8/12)
BlnI 31 CCTAGG C^CTAGG
BpuAI 553 GAAGAC GAAGAC(2/6)
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 27 GAGACC GGTCTC(1/5)
BsaWI 463 TCCGGA [AT]^CCGG[AT]
BseAI 463 TCCGGA T^CCGGA
BsiHKAI 41 GTGCTC G[AT]GC[AT]^C
BsmBI 8 GAGACG CGTCTC(1/5)
BspEI 463 TCCGGA T^CCGGA
BstBI 366 TTCGAA TT^CGAA
BstF5I 461 CATCC GGATG(2/0)
BstYI 59 AGATCC [GA]^GATC[CT]
EcoNI 17 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 211 ATGCAT ATGCA^T
Fnu4HI 516 GCAGC GC^[ACGT]GC
FokI 461 CATCC GGATG(9/13)
HapII 464 CCGG C^CGG
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HpaII 464 CCGG C^CGG
HphI 522 GGTGA GGTGA(8/7)
ItaI 516 GCAGC GC^[ACGT]GC
MaeII 285 ACGT A^CGT
MaeIII 263 GTAAC ^GT[ACGT]AC
MflI 59 AGATCC [GA]^GATC[CT]
MroI 463 TCCGGA T^CCGGA
MseI 452 TTAA T^TAA
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MspI 464 CCGG C^CGG
MwoI 336 GCCCAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 211 ATGCAT ATGCA^T
NspI 483 ACATGC [GA]CATG^[CT]
NspV 366 TTCGAA TT^CGAA
PvuII 517 CAGCTG CAG^CTG
RsaI 437 GTAC GT^AC
SfaNI 385 GCATC GCATC(5/9)
SfuI 366 TTCGAA TT^CGAA
Tru9I 452 TTAA T^TAA
TseI 516 GCAGC G^C[AT]GC
XhoII 59 AGATCC [GA]^GATC[CT]
XmnI 359 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 139 AGCT AG^CT
518 AGCT
AvaII 466 GGACC G^G[AT]CC
491 GGACC
BfaI 32 CTAG C^TAG
181 CTAG
BmyI 41 GTGCTC G[AGT]GC[ACT]^C
417 GTGCCC
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
Bsp1286I 41 GTGCTC G[AGT]GC[ACT]^C
417 GTGCCC
Cac8I 305 GCAGGC GC[ACGT]^[ACGT]GC
345 GCCAGC
EarI 153 GAAGAG CTCTTC(1/4)
532 GAAGAG
Ksp632I 153 GAAGAG CTCTTC(1/4)
532 GAAGAG
MaeI 32 CTAG C^TAG
181 CTAG
TaqI 74 TCGA T^CGA
367 TCGA
TthHB8I 74 TCGA T^CGA
367 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
BcgI BpmI BsaJI BsiYI BslI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII EcoT14I HaeIII HinfI MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TfiI