(Linear) (Six-base) MAPSORT of: KIBB7365.seq  Check: 1695  from: 1  to: 564



KIBB7365.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AvrII C'CTAG_G

Cuts at:      0      31     564 
   Size:         31     533
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     560     564 
   Size:        560       4
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35      64      98     315     349     357     391 
   Size:          1      34      29      34     217      34       8      34

Cuts at:    391     564 
   Size:        173

  Fragments arranged by size: 

                 217     173      34      34      34      34      29       8
                   1
 
BfrBI ATG'CAT

Cuts at:      0     213     564 
   Size:        213     351
 
Bme1580I G_kGCm'C

Cuts at:      0     421     564 
   Size:        421     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     295     407     564 
   Size:        220      75     112     157

  Fragments arranged by size: 

                 220     157     112      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     564 
   Size:         21     543
 
BsaHI Gr'CG_yC

Cuts at:      0      14     564 
   Size:         14     550
 
BsaWI w'CCGG_w

Cuts at:      0     463     564 
   Size:        463     101
 
BsiHKAI G_wGCw'C

Cuts at:      0      45     564 
   Size:         45     519
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       2     564 
   Size:          2     562
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     421     564 
   Size:         45     376     143

  Fragments arranged by size: 

                 376     143      45
 
BspEI T'CCGG_A

Cuts at:      0     463     564 
   Size:        463     101
 
BstBI TT'CG_AA

Cuts at:      0     367     564 
   Size:        367     197
 
BstYI r'GATC_y

Cuts at:      0      59     564 
   Size:         59     505
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     527     564 
   Size:        148     379      37

  Fragments arranged by size: 

                 379     148      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     295     407     564 
   Size:        220      75     112     157

  Fragments arranged by size: 

                 220     157     112      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     564 
   Size:         21     543
 
MspA1I CmG'CkG

Cuts at:      0     519     564 
   Size:        519      45
 
NsiI A_TGCA'T

Cuts at:      0     215     564 
   Size:        215     349
 
NspI r_CATG'y

Cuts at:      0     487     564 
   Size:        487      77
 
PfoI T'CCnGG_A

Cuts at:      0     423     564 
   Size:        423     141
 
PvuII CAG'CTG

Cuts at:      0     519     564 
   Size:        519      45
 
StyI C'CwwG_G

Cuts at:      0      31     125     267     564 
   Size:         31      94     142     297

  Fragments arranged by size: 

                 297     142      94      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     363     564 
   Size:        363     201
 

 Enzymes that do cut:

    AvrII     BbsI     BcgI    BfrBI Bme1580I     BpmI     BsaI    BsaHI
    BsaWI  BsiHKAI    BsmBI Bsp1286I    BspEI    BstBI    BstYI     EarI
  Eco57MI    EcoNI   MspA1I     NsiI     NspI     PfoI    PvuII     StyI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI