(Circular) (Six-base) MAPSORT of: pFN21AB7363.seq Check: 4727 from: 1 to: 5791 pFN21AB7363 5791 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 3084 3112 3084 Size: 28 5763 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3854 5238 5506 1632 Size: 2222 1384 268 1917 Fragments arranged by size: 2222 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 2366 1166 Size: 1200 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2666 1184 Size: 1482 4309 AlwNI CAG_nnn'CTG Cuts at: 2846 3534 5106 2846 Size: 688 1572 3531 Fragments arranged by size: 3531 1572 688 ApaI G_GGCC'C Cuts at: 3056 3056 Size: 5791 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 2508 2738 3100 3326 3478 5587 1493 Size: 1015 230 362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1015 362 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2592 2604 2764 2783 2983 3116 1870 Size: 73 649 12 160 19 200 133 4545 Fragments arranged by size: 4545 649 200 160 133 73 19 12 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2448 3084 3112 4567 618 Size: 325 217 627 661 636 28 1455 1842 Fragments arranged by size: 1842 1455 661 636 627 325 217 28 BanII G_rGCy'C Cuts at: 1391 2259 2588 2961 3056 3110 1391 Size: 868 329 373 95 54 4072 Fragments arranged by size: 4072 868 373 329 95 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3016 3050 3982 4016 1903 Size: 34 1079 34 932 34 3678 Fragments arranged by size: 3678 1079 932 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2334 5786 2334 Size: 3452 2339 BlpI GC'TnA_GC Cuts at: 2556 2587 3208 2556 Size: 31 621 5139 Fragments arranged by size: 5139 621 31 Bme1580I G_kGCm'C Cuts at: 1790 3056 3852 5008 1790 Size: 1266 796 1156 2573 Fragments arranged by size: 2573 1266 1156 796 BmgBI CAC'GTC Cuts at: 1318 2377 1318 Size: 1059 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2768 3522 4480 Size: 416 53 83 399 24 1351 754 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1351 958 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2278 2278 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2877 4451 1239 Size: 1638 1574 2579 Fragments arranged by size: 2579 1638 1574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2607 4454 882 Size: 617 1108 1847 2219 Fragments arranged by size: 2219 1847 1108 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 2072 5785 2072 Size: 3713 2078 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2415 2628 3598 3980 Size: 53 83 186 936 882 213 970 382 Cuts at: 3980 4615 275 Size: 635 1451 Fragments arranged by size: 1451 970 936 882 635 382 213 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 2558 2588 2869 Size: 30 165 30 1083 30 42 30 281 Cuts at: 2869 2899 1178 Size: 30 4070 Fragments arranged by size: 4070 1083 281 165 42 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5791 BseYI C'CCAG_C Cuts at: 2022 2638 2653 2906 3023 4994 5490 2022 Size: 616 15 253 117 1971 496 2323 Fragments arranged by size: 2323 1971 616 496 253 117 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2305 2961 3852 3937 5008 2259 Size: 46 656 891 85 1071 3042 Fragments arranged by size: 3042 1071 891 656 85 46 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2305 2588 2961 3056 3110 3852 Size: 399 469 46 283 373 95 54 742 Cuts at: 3852 3937 5008 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 373 283 95 85 54 46 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 2518 3680 5410 2518 Size: 1162 1730 2899 Fragments arranged by size: 2899 1730 1162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3146 844 Size: 2302 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2752 3678 1394 Size: 429 929 926 3507 Fragments arranged by size: 3507 929 926 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2445 3029 4435 4622 1700 Size: 110 635 584 1406 187 2869 Fragments arranged by size: 2869 1406 635 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 2252 3851 4863 2252 Size: 1599 1012 3180 Fragments arranged by size: 3180 1599 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 2657 1184 Size: 1473 4318 BstXI CCAn_nnnn'nTGG Cuts at: 3047 3047 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2180 2334 3121 3488 3873 3890 5342 5428 Size: 208 154 787 367 385 17 1452 86 Cuts at: 5428 5440 5786 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 787 385 367 346 208 154 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2280 2280 Size: 5791 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 2949 513 Size: 552 939 360 585 3355 Fragments arranged by size: 3355 939 585 552 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 2805 3307 4101 4121 2805 Size: 502 794 20 4475 Fragments arranged by size: 4475 794 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2987 4798 809 Size: 507 1671 1811 1802 Fragments arranged by size: 1811 1802 1671 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2952 2970 Size: 54 1425 50 275 18 295 827 18 Cuts at: 2970 3037 3238 4127 5756 8 Size: 67 201 889 1629 43 Fragments arranged by size: 1629 1425 889 827 295 275 201 67 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3721 1353 Size: 2368 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2959 2257 Size: 702 5089 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2877 3854 4451 5238 5506 1239 Size: 393 1245 977 597 787 268 1524 Fragments arranged by size: 1524 1245 977 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2164 3052 3053 2164 Size: 888 1 4902 Fragments arranged by size: 4902 888 1 EcoRI G'AATT_C Cuts at: 2738 3100 3478 2738 Size: 362 378 5051 Fragments arranged by size: 5051 378 362 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2353 2305 Size: 48 5743 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2772 3009 4938 1678 Size: 1094 237 1929 2531 Fragments arranged by size: 2531 1929 1094 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2337 2558 2590 2851 Size: 32 135 32 733 32 221 32 261 Cuts at: 2851 2869 2883 2901 4432 4464 4506 4538 1373 Size: 18 14 18 1531 32 42 32 2626 Fragments arranged by size: 2626 1531 733 261 221 135 42 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3088 3116 3088 Size: 28 5763 MfeI C'AATT_G Cuts at: 2271 3390 2271 Size: 1119 4672 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3969 Fragments arranged by size: 3969 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2366 2423 2517 2648 3749 4108 Size: 648 1041 159 57 94 131 1101 359 Cuts at: 4108 4267 518 Size: 159 2042 Fragments arranged by size: 2042 1101 1041 648 359 159 159 131 94 57 MspA1I CmG'CkG Cuts at: 1606 2834 2843 3207 3884 5032 5277 1606 Size: 1228 9 364 677 1148 245 2120 Fragments arranged by size: 2120 1228 1148 677 364 245 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2949 513 Size: 552 939 945 3355 Fragments arranged by size: 3355 945 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2653 3149 4694 1132 Size: 97 1424 496 1545 2229 Fragments arranged by size: 2229 1545 1424 496 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5791 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 5791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PspOMI G'GGCC_C Cuts at: 3052 3052 Size: 5791 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 SacI G_AGCT'C Cuts at: 2259 2961 2259 Size: 702 5089 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SanDI GG'GwC_CC Cuts at: 2164 2164 Size: 5791 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 3139 4274 4955 5146 826 Size: 219 2094 1135 681 191 1471 Fragments arranged by size: 2094 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 2594 3118 1872 Size: 722 524 4545 Fragments arranged by size: 4545 722 524 SmlI C'TyrA_G Cuts at: 820 1017 1943 2604 3895 4796 5058 820 Size: 197 926 661 1291 901 262 1553 Fragments arranged by size: 1553 1291 926 901 661 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 2653 3149 1132 Size: 1521 496 3774 Fragments arranged by size: 3774 1521 496 SspI AAT'ATT Cuts at: 5 52 2387 3715 5 Size: 47 2335 1328 2081 Fragments arranged by size: 2335 2081 1328 47 StuI AGG'CCT Cuts at: 1517 2706 1517 Size: 1189 4602 StyI C'CwwG_G Cuts at: 513 1065 2004 2949 3230 513 Size: 552 939 945 281 3074 Fragments arranged by size: 3074 945 939 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3818 3835 3988 4173 4618 325 Size: 1255 2238 17 153 185 445 1498 Fragments arranged by size: 2238 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 5751 5783 2486 Size: 32 3233 32 2494 Fragments arranged by size: 3233 2494 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 2604 1943 Size: 661 5130 XmaI C'CCGG_G Cuts at: 1870 2592 3116 1870 Size: 722 524 4545 Fragments arranged by size: 4545 722 524 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI