Restriction map for
pFN21AB7363
(Linear) (Six-base) MAPSORT of: KIBB7363.seq Check: 3819 from: 1 to: 1086
KIBB7363.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
Acc65I G'GTAC_C
Cuts at: 0 1079 1086
Size: 1079 7
AleI CACnn'nnGTG
Cuts at: 0 361 1086
Size: 361 725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 1086
Size: 661 425
AlwNI CAG_nnn'CTG
Cuts at: 0 841 1086
Size: 841 245
ApaI G_GGCC'C
Cuts at: 0 1051 1086
Size: 1051 35
ApoI r'AATT_y
Cuts at: 0 503 733 1086
Size: 503 230 353
Fragments arranged by size:
503 353 230
AvaI C'yCGr_G
Cuts at: 0 587 599 759 778 978 1086
Size: 587 12 160 19 200 108
Fragments arranged by size:
587 200 160 108 19 12
BanI G'GyrC_C
Cuts at: 0 443 1079 1086
Size: 443 636 7
Fragments arranged by size:
636 443 7
BanII G_rGCy'C
Cuts at: 0 254 583 956 1051 1086
Size: 254 329 373 95 35
Fragments arranged by size:
373 329 254 95 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1011 1045 1086
Size: 1011 34 41
Fragments arranged by size:
1011 41 34
BglII A'GATC_T
Cuts at: 0 329 1086
Size: 329 757
BlpI GC'TnA_GC
Cuts at: 0 551 582 1086
Size: 551 31 504
Fragments arranged by size:
551 504 31
Bme1580I G_kGCm'C
Cuts at: 0 1051 1086
Size: 1051 35
BmgBI CAC'GTC
Cuts at: 0 372 1086
Size: 372 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 763 1086
Size: 763 323
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 273 1086
Size: 273 813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1086
Size: 872 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 602 1086
Size: 602 484
BsaBI GATnn'nnATC
Cuts at: 0 67 1086
Size: 67 1019
BsaHI Gr'CG_yC
Cuts at: 0 410 623 1086
Size: 410 213 463
Fragments arranged by size:
463 410 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 553 583 864 894 1086
Size: 481 30 42 30 281 30 192
Fragments arranged by size:
481 281 192 42 30 30 30
BseYI C'CCAG_C
Cuts at: 0 17 633 648 901 1018 1086
Size: 17 616 15 253 117 68
Fragments arranged by size:
616 253 117 68 17 15
BsiHKAI G_wGCw'C
Cuts at: 0 254 300 956 1086
Size: 254 46 656 130
Fragments arranged by size:
656 254 130 46
Bsp1286I G_dGCh'C
Cuts at: 0 254 300 583 956 1051 1086
Size: 254 46 283 373 95 35
Fragments arranged by size:
373 283 254 95 46 35
BspHI T'CATG_A
Cuts at: 0 513 1086
Size: 513 573
BsrBI CCG'CTC
Cuts at: 0 747 1086
Size: 747 339
BsrFI r'CCGG_y
Cuts at: 0 440 1024 1086
Size: 440 584 62
Fragments arranged by size:
584 440 62
BssSI C'ACGA_G
Cuts at: 0 247 1086
Size: 247 839
BstEII G'GTnAC_C
Cuts at: 0 652 1086
Size: 652 434
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1042 1086
Size: 1042 44
BstYI r'GATC_y
Cuts at: 0 175 329 1086
Size: 175 154 757
Fragments arranged by size:
757 175 154
Bsu36I CC'TnA_GG
Cuts at: 0 275 1086
Size: 275 811
BtgI C'CryG_G
Cuts at: 0 359 944 1086
Size: 359 585 142
Fragments arranged by size:
585 359 142
BtsI GCAGTG_nn'
Cuts at: 0 800 1086
Size: 800 286
DrdI GACnn_nn'nnGTC
Cuts at: 0 982 1086
Size: 982 104
EaeI y'GGCC_r
Cuts at: 0 120 947 965 1032 1086
Size: 120 827 18 67 54
Fragments arranged by size:
827 120 67 54 18
EcoICRI GAG'CTC
Cuts at: 0 252 954 1086
Size: 252 702 132
Fragments arranged by size:
702 252 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1086
Size: 872 214
EcoO109I rG'GnC_Cy
Cuts at: 0 159 1047 1048 1086
Size: 159 888 1 38
Fragments arranged by size:
888 159 38 1
EcoRI G'AATT_C
Cuts at: 0 733 1086
Size: 733 353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 348 1086
Size: 300 48 738
Fragments arranged by size:
738 300 48
HaeII r_GCGC'y
Cuts at: 0 767 1004 1086
Size: 767 237 82
Fragments arranged by size:
767 237 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 553 585 846 864 878 896
Size: 300 32 221 32 261 18 14 18
Cuts at: 896 1086
Size: 190
Fragments arranged by size:
300 261 221 190 32 32 18 18
14
KpnI G_GTAC'C
Cuts at: 0 1083 1086
Size: 1083 3
MfeI C'AATT_G
Cuts at: 0 266 1086
Size: 266 820
MslI CAynn'nnrTG
Cuts at: 0 202 361 418 512 643 1086
Size: 202 159 57 94 131 443
Fragments arranged by size:
443 202 159 131 94 57
MspA1I CmG'CkG
Cuts at: 0 829 838 1086
Size: 829 9 248
Fragments arranged by size:
829 248 9
NcoI C'CATG_G
Cuts at: 0 944 1086
Size: 944 142
NspI r_CATG'y
Cuts at: 0 648 1086
Size: 648 438
PpuMI rG'GwC_Cy
Cuts at: 0 159 1086
Size: 159 927
PspOMI G'GGCC_C
Cuts at: 0 1047 1086
Size: 1047 39
SacI G_AGCT'C
Cuts at: 0 254 956 1086
Size: 254 702 130
Fragments arranged by size:
702 254 130
SanDI GG'GwC_CC
Cuts at: 0 159 1086
Size: 159 927
SmaI CCC'GGG
Cuts at: 0 589 1086
Size: 589 497
SmlI C'TyrA_G
Cuts at: 0 599 1086
Size: 599 487
SphI G_CATG'C
Cuts at: 0 648 1086
Size: 648 438
SspI AAT'ATT
Cuts at: 0 382 1086
Size: 382 704
StuI AGG'CCT
Cuts at: 0 701 1086
Size: 701 385
StyI C'CwwG_G
Cuts at: 0 944 1086
Size: 944 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 1086
Size: 481 32 573
Fragments arranged by size:
573 481 32
XhoI C'TCGA_G
Cuts at: 0 599 1086
Size: 599 487
XmaI C'CCGG_G
Cuts at: 0 587 1086
Size: 587 499
Enzymes that do cut:
Acc65I AleI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BcgI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaBI BsaHI BsaXI BseYI BsiHKAI Bsp1286I
BspHI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I KpnI MfeI MslI MspA1I NcoI
NspI PpuMI PspOMI SacI SanDI SmaI SmlI SphI
SspI StuI StyI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BmtI Bpu10I BpuEI BsaAI BsaWI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EarI EciI EcoNI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI TaqII
TatI Tth111I XbaI XcmI XmnI ZraI