Restriction map for
pFN21AB7301
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BsiEI BsiWI BsmBI BsmFI BsmI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I CpoI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HgaI
HindIII KpnI KspI MfeI MluI
MluNI MscI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 1251 GACGTC GACGT^C
AccI 1312 GTAGAC GT^[AC][GT]AC
AccIII 1326 TCCGGA T^CCGGA
AciI 33 GCGG CCGC(-3/-1)
AflIII 1039 ACACGT A^C[GA][CT]GT
AhdI 1246 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1438 CCTAAGG CC^T[ACGT]AGG
AseI 2241 ATTAAT AT^TAAT
AsnI 2241 ATTAAT AT^TAAT
Asp700I 2160 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1246 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2188 GAGCAC G[AT]GC[AT]^C
AvaI 57 CCCGAG C^[CT]CG[GA]G
BbeI 170 GGCGCC GGCGC^C
BclI 1542 TGATCA T^GATCA
BcnI 2381 CCCGG CC^[GC]GG
BglII 1395 AGATCT A^GATCT
BmyI 2188 GAGCAC G[AGT]GC[ACT]^C
BsaBI 232 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1326 TCCGGA [AT]^CCGG[AT]
BseAI 1326 TCCGGA T^CCGGA
BsgI 1841 GTGCAG GTGCAG(16/14)
BsiHKAI 2188 GAGCAC G[AT]GC[AT]^C
BsiYI 1120 CCCAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1120 CCCAAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 57 CCCGAG C^[CT]CG[GA]G
Bsp1286I 2188 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 2294 TGTACA T^GTACA
BspDI 313 ATCGAT AT^CGAT
BspEI 1326 TCCGGA T^CCGGA
BspMI 1758 GCAGGT ACCTGC(4/8)
BsrGI 2294 TGTACA T^GTACA
BssSI 1022 CTCGTG CACGAG(-5/-1)
BstAPI 1749 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1438 CCTAAGG CC^T[ACGT]AGG
CfoI 171 GCGC GCG^C
ClaI 313 ATCGAT AT^CGAT
DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 155 CCGTGG C^C[GA][CT]GG
Eam1105I 1246 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1438 CCTAAGG CC^T[ACGT]AGG
EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1891 CCTTGG C^C[AT][AT]GG
FbaI 1542 TGATCA T^GATCA
HaeII 170 GGCGCC [GA]GCGC^[CT]
HhaI 171 GCGC GCG^C
HinP1I 171 GCGC G^CGC
HincII 2269 GTTAAC GT[CT]^[GA]AC
HindII 2269 GTTAAC GT[CT]^[GA]AC
HpaI 2269 GTTAAC GTT^AAC
KasI 170 GGCGCC G^GCGCC
MamI 232 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1326 TCCGGA T^CCGGA
MspA1I 31 CAGCGG C[AC]G^C[GT]G
NarI 170 GGCGCC GG^CGCC
NciI 2381 CCCGG CC^[GC]GG
NspI 990 GCATGC [GA]CATG^[CT]
PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2241 ATTAAT AT^TAAT
ScaI 1295 AGTACT AGT^ACT
SfoI 170 GGCGCC GGC^GCC
SmlI 2368 CTTGAG C^T[CT][GA]AG
SpeI 931 ACTAGT A^CTAGT
SphI 990 GCATGC GCATG^C
SspBI 2294 TGTACA T^GTACA
StyI 1891 CCTTGG C^C[AT][AT]GG
XbaI 1255 TCTAGA T^CTAGA
XcmI 1237 CCAGAAAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2160 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 170 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
AfaI 1296 GTAC GT^AC
2295 GTAC
AlwNI 224 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2194 CAGAATCTG
BanI 170 GGCGCC G^G[CT][GA]CC
571 GGCACC
BpmI 2081 CTCCAG CTGGAG(16/14)
715 CTGGAG
BsaHI 170 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
BsaJI 155 CCGTGG C^C[ACGT][ACGT]GG
1891 CCTTGG
BsmAI 659 GAGAC GTCTC(1/5)
1537 GAGAC
BsrDI 1501 GCAATG GCAATG(2/0)
2290 GCAATG
BstNI 1365 CCTGG CC^[AT]GG
1825 CCAGG
BstYI 1395 AGATCT [GA]^GATC[CT]
2328 AGATCC
Cac8I 990 GCATGC GC[ACGT]^[ACGT]GC
1095 GCTGGC
DraI 1114 TTTAAA TTT^AAA
1576 TTTAAA
EcoRII 1365 CCTGG ^CC[AT]GG
1825 CCAGG
HapII 1327 CCGG C^CGG
2382 CCGG
Hin1I 170 GGCGCC G[GA]^CG[CT]C
1251 GACGTC
HpaII 1327 CCGG C^CGG
2382 CCGG
MaeII 1041 ACGT A^CGT
1252 ACGT
MflI 1395 AGATCT [GA]^GATC[CT]
2328 AGATCC
MspI 1327 CCGG C^CGG
2382 CCGG
MvaI 1365 CCTGG CC^[AT]GG
1825 CCAGG
RsaI 1296 GTAC GT^AC
2295 GTAC
SapI 1186 GAAGAGC GCTCTTC(1/4)
1585 GAAGAGC
SspI 1134 AATATT AAT^ATT
1484 AATATT
XhoII 1395 AGATCT [GA]^GATC[CT]
2328 AGATCC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BfaI BseRI BspHI BsrI
BssKI BstF5I Cfr13I DdeI DpnII
EarI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV PleI RcaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I