(Circular) (Six-base) MAPSORT of: pFN21AB7300.seq Check: 787 from: 1 to: 7573 pFN21AB7300 7573 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4426 4758 4426 Size: 332 7241 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5383 278 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3837 4916 1937 Size: 1900 1079 4594 Fragments arranged by size: 4594 1900 1079 Acc65I G'GTAC_C Cuts at: 3515 4745 4894 3515 Size: 1230 149 6194 Fragments arranged by size: 6194 1230 149 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2148 2555 3981 4240 5636 7020 7288 1632 Size: 516 407 1426 259 1396 1384 268 1917 Fragments arranged by size: 1917 1426 1396 1384 516 407 268 259 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 2808 6472 7449 1203 Size: 1605 3664 977 1327 Fragments arranged by size: 3664 1605 1327 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 6302 6302 Size: 7573 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2283 3103 4558 1184 Size: 1099 820 1455 4199 Fragments arranged by size: 4199 1455 1099 820 AlwNI CAG_nnn'CTG Cuts at: 2562 3589 5316 6888 2562 Size: 1027 1727 1572 3247 Fragments arranged by size: 3247 1727 1572 1027 ApaI G_GGCC'C Cuts at: 4039 4470 4039 Size: 431 7142 ApaLI G'TGCA_C Cuts at: 5630 6786 5630 Size: 1156 6417 ApoI r'AATT_y Cuts at: 1493 4882 5108 5260 7369 1493 Size: 3389 226 152 2109 1697 Fragments arranged by size: 3389 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2906 6127 160 Size: 872 1874 3221 1606 Fragments arranged by size: 3221 1874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 2518 4469 4898 1870 Size: 73 575 1951 429 4545 Fragments arranged by size: 4545 1951 575 429 73 BamHI G'GATC_C Cuts at: 4903 5270 4903 Size: 367 7206 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2930 3138 3515 3775 Size: 325 217 627 234 909 208 377 260 Cuts at: 3775 3827 4745 4864 4894 6349 618 Size: 52 918 119 30 1455 1842 Fragments arranged by size: 1842 1455 918 909 627 377 325 260 234 217 208 119 52 30 BanII G_rGCy'C Cuts at: 1391 2208 2460 2569 3607 3746 4039 4470 4758 Size: 817 252 109 1038 139 293 431 288 Cuts at: 4758 4892 1391 Size: 134 4072 Fragments arranged by size: 4072 1038 817 431 293 288 252 139 134 109 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 7573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 928 Size: 971 266 6336 Fragments arranged by size: 6336 971 266 BbvCI CC'TCA_GC Cuts at: 2220 2441 4213 4232 4674 2220 Size: 221 1772 19 442 5119 Fragments arranged by size: 5119 1772 442 221 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2920 2954 5764 5798 1903 Size: 34 50 34 899 34 2810 34 3678 Fragments arranged by size: 3678 2810 899 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5465 6675 5465 Size: 1210 6363 BclI T'GATC_A Cuts at: 1548 4335 1548 Size: 2787 4786 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 3035 3101 Size: 107 122 71 631 605 393 970 66 Cuts at: 3101 4029 4068 6184 136 Size: 928 39 2116 1525 Fragments arranged by size: 2116 1525 970 928 631 605 393 122 107 71 66 39 BglII A'GATC_T Cuts at: 2495 7568 2495 Size: 5073 2500 BlpI GC'TnA_GC Cuts at: 2202 2393 4990 2202 Size: 191 2597 4785 Fragments arranged by size: 4785 2597 191 Bme1580I G_kGCm'C Cuts at: 1790 3141 3780 3830 4039 4470 4869 5634 6790 Size: 1351 639 50 209 431 399 765 1156 Cuts at: 6790 1790 Size: 2573 Fragments arranged by size: 2573 1351 1156 765 639 431 399 209 50 BmgBI CAC'GTC Cuts at: 1318 3835 1318 Size: 2517 5056 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2572 3144 4598 Size: 416 53 83 399 24 1155 572 1454 Cuts at: 4598 5304 6262 442 Size: 706 958 1753 Fragments arranged by size: 1753 1454 1155 958 706 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7573 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4527 4527 Size: 7573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3003 3782 4143 4374 4549 4742 6233 Size: 1028 736 779 361 231 175 193 1491 Cuts at: 6233 1239 Size: 2579 Fragments arranged by size: 2579 1491 1028 779 736 361 231 193 175 Bpu10I CC'TnA_GC Cuts at: 2207 2220 2441 4213 4232 4439 4674 2207 Size: 13 221 1772 19 207 235 5106 Fragments arranged by size: 5106 1772 235 221 207 19 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 4190 4715 5698 6563 6861 2492 Size: 1698 525 983 865 298 3204 Fragments arranged by size: 3204 1698 983 865 525 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2685 4479 6236 882 Size: 617 1186 1794 1757 2219 Fragments arranged by size: 2219 1794 1757 1186 617 BsaAI yAC'GTr Cuts at: 493 1204 5364 493 Size: 711 4160 2702 Fragments arranged by size: 4160 2702 711 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2700 4313 5380 Size: 53 83 186 936 489 678 1613 1067 Cuts at: 5380 5762 6397 275 Size: 382 635 1451 Fragments arranged by size: 1613 1451 1067 936 678 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 4345 6006 6404 6678 6825 7236 2766 Size: 1579 1661 398 274 147 411 3103 Fragments arranged by size: 3103 1661 1579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4116 4146 1178 Size: 30 165 30 2713 30 4605 Fragments arranged by size: 4605 2713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3000 3866 3869 4365 4500 1186 Size: 1814 866 3 496 135 4259 Fragments arranged by size: 4259 1814 866 496 135 3 BseYI C'CCAG_C Cuts at: 4032 4368 6776 7272 4032 Size: 336 2408 496 4333 Fragments arranged by size: 4333 2408 496 336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3955 4404 1163 Size: 573 2219 449 4332 Fragments arranged by size: 4332 2219 573 449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 4577 5023 5784 6812 1540 Size: 275 186 68 2508 446 761 1028 2301 Fragments arranged by size: 2508 2301 1028 761 446 275 186 68 BsiHKAI G_wGCw'C Cuts at: 2208 2541 2969 3379 3746 5634 5719 6790 2208 Size: 333 428 410 367 1888 85 1071 2991 Fragments arranged by size: 2991 1888 1071 428 410 367 333 85 BsmI GAATG_Cn' Cuts at: 3016 5084 5177 3016 Size: 2068 93 5412 Fragments arranged by size: 5412 2068 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2460 2541 2569 2969 3141 3379 Size: 399 418 252 81 28 400 172 238 Cuts at: 3379 3607 3746 3780 3830 4039 4470 4758 4869 Size: 228 139 34 50 209 431 288 111 Cuts at: 4869 4892 5634 5719 6790 1391 Size: 23 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 431 418 400 399 288 252 238 228 209 172 139 111 85 81 50 34 28 23 BspEI T'CCGG_A Cuts at: 2766 4345 7236 2766 Size: 1579 2891 3103 Fragments arranged by size: 3103 2891 1579 BspHI T'CATG_A Cuts at: 3798 5462 7192 3798 Size: 1664 1730 4179 Fragments arranged by size: 4179 1730 1664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3619 4426 4758 4928 844 Size: 2775 807 332 170 3489 Fragments arranged by size: 3489 2775 807 332 170 BsrBI CCG'CTC Cuts at: 1394 1823 5460 1394 Size: 429 3637 3507 Fragments arranged by size: 3637 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3343 3988 6068 6242 65 Size: 1183 2095 645 2080 174 1396 Fragments arranged by size: 2095 2080 1396 1183 645 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4518 6217 6404 1700 Size: 110 2708 1699 187 2869 Fragments arranged by size: 2869 2708 1699 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7573 BssSI C'ACGA_G Cuts at: 2654 2777 3041 5633 6645 2654 Size: 123 264 2592 1012 3582 Fragments arranged by size: 3582 2592 1012 264 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 4700 4798 794 Size: 3906 98 3569 Fragments arranged by size: 3906 3569 98 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 2306 1184 Size: 1122 6451 BstXI CCAn_nnnn'nTGG Cuts at: 2944 3022 3059 3512 2944 Size: 78 37 453 7005 Fragments arranged by size: 7005 453 78 37 BstYI r'GATC_y Cuts at: 1972 2495 3729 3813 4903 5270 5655 5672 7124 Size: 523 1234 84 1090 367 385 17 1452 Cuts at: 7124 7210 7222 7568 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1234 1090 523 385 367 346 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 3356 3849 3356 Size: 493 7080 BtgI C'CryG_G Cuts at: 513 1065 2069 2353 2800 2926 3033 3988 4063 Size: 552 1004 284 447 126 107 955 75 Cuts at: 4063 4718 513 Size: 655 3368 Fragments arranged by size: 3368 1004 955 655 552 447 284 126 107 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6834 Fragments arranged by size: 6834 683 56 BtsI GCAGTG_nn' Cuts at: 2341 3452 3484 3584 3938 4018 4424 4649 5089 Size: 1111 32 100 354 80 406 225 440 Cuts at: 5089 5883 5903 2341 Size: 794 20 4011 Fragments arranged by size: 4011 1111 794 440 406 354 225 100 80 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 2625 4877 2625 Size: 2252 5321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2377 4495 6580 809 Size: 507 1061 2118 2085 1802 Fragments arranged by size: 2118 2085 1802 1061 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2958 3093 Size: 54 1425 50 275 18 236 892 135 Cuts at: 3093 3532 3985 4134 4520 4574 5020 5909 7538 Size: 439 453 149 386 54 446 889 1629 Cuts at: 7538 8 Size: 43 Fragments arranged by size: 1629 1425 892 889 453 446 439 386 275 236 149 135 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2066 4574 5020 1537 Size: 275 254 2508 446 4090 Fragments arranged by size: 4090 2508 446 275 254 EarI CTCTTCn'nnn_ Cuts at: 1353 2574 3285 3306 5503 1353 Size: 1221 711 21 2197 3423 Fragments arranged by size: 3423 2197 1221 711 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3383 6160 6534 6680 387 Size: 1064 138 1794 2777 374 146 1280 Fragments arranged by size: 2777 1794 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2206 3744 2206 Size: 1538 6035 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2267 2555 3003 3782 3981 4143 Size: 393 516 119 288 448 779 199 162 Cuts at: 4143 4240 4374 4549 4742 5636 6233 7020 7288 Size: 97 134 175 193 894 597 787 268 Cuts at: 7288 1239 Size: 1524 Fragments arranged by size: 1524 894 787 779 597 516 448 393 288 268 199 193 175 162 134 119 97 EcoO109I rG'GnC_Cy Cuts at: 3420 3596 3845 4036 4109 4410 4466 3420 Size: 176 249 191 73 301 56 6527 Fragments arranged by size: 6527 301 249 191 176 73 56 EcoRI G'AATT_C Cuts at: 4882 5260 4882 Size: 378 7195 FspI TGC'GCA Cuts at: 6079 6079 Size: 7573 HaeII r_GCGC'y Cuts at: 1678 2025 6720 1678 Size: 347 4695 2531 Fragments arranged by size: 4695 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2220 2252 2612 2644 3074 Size: 32 135 32 648 32 360 32 430 Cuts at: 3074 3106 3721 3753 4093 4125 4327 4359 6214 Size: 32 615 32 340 32 202 32 1855 Cuts at: 6214 6246 6288 6320 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1855 648 615 430 360 340 202 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2992 4917 5163 669 Size: 988 281 1054 1925 246 3079 Fragments arranged by size: 3079 1925 1054 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KasI G'GCGC_C Cuts at: 2021 2021 Size: 7573 KpnI G_GTAC'C Cuts at: 3519 4749 4898 3519 Size: 1230 149 6194 Fragments arranged by size: 6194 1230 149 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2840 3246 3792 6687 6871 1305 Size: 1535 406 546 2895 184 2007 Fragments arranged by size: 2895 2007 1535 546 406 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 3987 4136 10 Size: 54 1425 343 1128 1027 149 3447 Fragments arranged by size: 3447 1425 1128 1027 343 149 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 2960 3020 3239 4142 4163 5531 Size: 648 1749 45 60 219 903 21 1368 Cuts at: 5531 5890 6049 518 Size: 359 159 2042 Fragments arranged by size: 2042 1749 1368 903 648 359 219 159 60 45 21 MspA1I CmG'CkG Cuts at: 1606 2071 3128 3589 4275 4871 4989 5666 6814 Size: 465 1057 461 686 596 118 677 1148 Cuts at: 6814 7059 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1057 686 677 596 465 461 245 118 NaeI GCC'GGC Cuts at: 4520 4520 Size: 7573 NarI GG'CG_CC Cuts at: 2022 2022 Size: 7573 NcoI C'CATG_G Cuts at: 513 1065 3033 3988 513 Size: 552 1968 955 4098 Fragments arranged by size: 4098 1968 955 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7573 NgoMIV G'CCGG_C Cuts at: 4518 4518 Size: 7573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7573 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 2812 2920 3229 3559 3868 4931 6476 Size: 97 1583 108 309 330 309 1063 1545 Cuts at: 6476 1132 Size: 2229 Fragments arranged by size: 2229 1583 1545 1063 330 309 309 108 97 PasI CC'CwG_GG Cuts at: 1348 3875 4318 4463 1348 Size: 2527 443 145 4458 Fragments arranged by size: 4458 2527 443 145 PciI A'CATG_T Cuts at: 2808 6472 2808 Size: 3664 3909 PflMI CCAn_nnn'nTGG Cuts at: 1108 2562 3046 1108 Size: 1454 484 5635 Fragments arranged by size: 5635 1454 484 PfoI T'CCnGG_A Cuts at: 2238 3456 4499 2238 Size: 1218 1043 5312 Fragments arranged by size: 5312 1218 1043 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 7155 7187 5642 Size: 32 1481 32 6028 Fragments arranged by size: 6028 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3420 3596 3845 4109 4410 3420 Size: 176 249 264 301 6583 Fragments arranged by size: 6583 301 264 249 176 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7573 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 4035 4466 4035 Size: 431 7142 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2130 2162 2130 Size: 32 7541 PstI C_TGCA'G Cuts at: 830 2058 2133 3082 3198 4181 4311 4925 830 Size: 1228 75 949 116 983 130 614 3478 Fragments arranged by size: 3478 1228 983 949 614 130 116 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 PvuII CAG'CTG Cuts at: 3128 3589 3128 Size: 461 7112 SacI G_AGCT'C Cuts at: 2208 3746 2208 Size: 1538 6035 SacII CC_GC'GG Cuts at: 2072 2072 Size: 7573 SalI G'TCGA_C Cuts at: 1936 4915 1936 Size: 2979 4594 SanDI GG'GwC_CC Cuts at: 3420 3596 4410 3420 Size: 176 814 6583 Fragments arranged by size: 6583 814 176 SapI GCTCTTCn'nnn_ Cuts at: 2574 2574 Size: 7573 SbfI CC_TGCA'GG Cuts at: 4925 4925 Size: 7573 ScaI AGT'ACT Cuts at: 5821 7533 5821 Size: 1712 5861 SfcI C'TryA_G Cuts at: 826 1045 2054 2129 3078 3194 4177 4307 4921 Size: 219 1009 75 949 116 983 130 614 Cuts at: 4921 6056 6737 6928 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 983 949 681 614 219 191 130 116 75 SfoI GGC'GCC Cuts at: 2023 2023 Size: 7573 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 2520 4471 4900 1872 Size: 648 1951 429 4545 Fragments arranged by size: 4545 1951 648 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 4205 4730 5677 6578 6840 Size: 197 926 564 1698 525 947 901 262 Cuts at: 6840 820 Size: 1553 Fragments arranged by size: 1698 1553 947 926 901 564 525 262 197 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 4931 1132 Size: 3799 3774 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 3539 4507 4570 1517 Size: 2022 968 63 4520 Fragments arranged by size: 4520 2022 968 63 StyI C'CwwG_G Cuts at: 513 1065 3033 3988 4838 5012 513 Size: 552 1968 955 850 174 3074 Fragments arranged by size: 3074 1968 955 850 552 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3102 3845 5600 5617 5770 5955 6400 Size: 1255 1522 743 1755 17 153 185 445 Cuts at: 6400 325 Size: 1498 Fragments arranged by size: 1755 1522 1498 1255 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4167 5819 7531 Size: 274 80 33 51 1443 2190 1652 1712 Cuts at: 7531 96 Size: 138 Fragments arranged by size: 2190 1712 1652 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2797 2829 4116 4148 7533 7565 2797 Size: 32 1287 32 3385 32 2805 Fragments arranged by size: 3385 2805 1287 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4492 1316 Size: 3176 4397 XbaI T'CTAG_A Cuts at: 4909 4909 Size: 7573 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2638 2941 4144 2329 Size: 309 303 1203 5758 Fragments arranged by size: 5758 1203 309 303 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 2518 4469 4898 1870 Size: 648 1951 429 4545 Fragments arranged by size: 4545 1951 648 429 XmnI GAAnn'nnTTC Cuts at: 5702 5702 Size: 7573 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5381 276 Size: 53 83 186 936 3847 2468 Fragments arranged by size: 3847 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SexAI SfiI SrfI SwaI