Restriction map for
pFN21AB7297
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BfaI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RsrII
SalI SapI SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SwaI
TfiI Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 609 ACATGT A^C[GA][CT]GT
AspI 3040 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1656 TGGCCA TGG^CCA
BbvCI 1181 CCTCAGC CCTCAGC(-5/-2)
BclI 932 TGATCA T^GATCA
BsiEI 3237 CGATCG CG[GA][CT]^CG
Bsp1407I 458 TGTACA T^GTACA
BspLU11I 609 ACATGT A^CATGT
BsrGI 458 TGTACA T^GTACA
BssHII 14 GCGCGC G^CGCGC
BstEII 2286 GGTCACC G^GT[ACGT]ACC
BstPI 2286 GGTCACC G^GT[ACGT]ACC
EarI 2682 GAAGAG CTCTTC(1/4)
EcoO65I 2286 GGTCACC G^GT[ACGT]ACC
EcoRV 3177 GATATC GAT^ATC
FbaI 932 TGATCA T^GATCA
Ksp632I 2682 GAAGAG CTCTTC(1/4)
KspI 62 CCGCGG CCGC^GG
MluNI 1656 TGGCCA TGG^CCA
MscI 1656 TGGCCA TGG^CCA
MseI 3170 TTAA T^TAA
NdeI 1652 CATATG CA^TATG
PflFI 3040 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3237 CGATCG CGAT^CG
SacII 62 CCGCGG CCGC^GG
SgrAI 2701 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3559 CCCGGG CCC^GGG
SphI 756 GCATGC GCATG^C
SspBI 458 TGTACA T^GTACA
SspI 913 AATATT AAT^ATT
Tru9I 3170 TTAA T^TAA
Tth111I 3040 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3559 CCCGGG C^CCGGG
XmaI 3559 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Alw44I 1595 GTGCAC G^TGCAC
1885 GTGCAC
ApaLI 1595 GTGCAC G^TGCAC
1885 GTGCAC
BamHI 391 GGATCC G^GATCC
2606 GGATCC
BbeI 305 GGCGCC GGCGC^C
1284 GGCGCC
BbrPI 163 CACGTG CAC^GTG
1213 CACGTG
BbsI 3628 GAAGAC GAAGAC(2/6)
3214 GTCTTC
BcgI 2902 CGAGTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
999 GCAGGTGTGTCG
BciVI 2804 GGATAC GTATCC(6/5)
3474 GGATAC
BlpI 1048 GCTGAGC GC^T[ACGT]AGC
1344 GCTCAGC
Bpu1102I 1048 GCTGAGC GC^T[ACGT]AGC
1344 GCTCAGC
BpuAI 3628 GAAGAC GAAGAC(2/6)
3214 GTCTTC
BsaAI 163 CACGTG [CT]AC^GT[GA]
1213 CACGTG
BsrBI 571 GAGCGG CCGCTC(-3/-3)
742 GAGCGG
BsrDI 2424 CATTGC GCAATG(2/0)
3019 CATTGC
CelII 1048 GCTGAGC GC^T[ACGT]AGC
1344 GCTCAGC
EcoNI 993 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1290 CCTGGTGCAGG
KasI 305 GGCGCC G^GCGCC
1284 GGCGCC
NarI 305 GGCGCC GG^CGCC
1284 GGCGCC
NcoI 822 CCATGG C^CATGG
2348 CCATGG
PmaCI 163 CACGTG CAC^GTG
1213 CACGTG
PmlI 163 CACGTG CAC^GTG
1213 CACGTG
SfoI 305 GGCGCC GGC^GCC
1284 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII