Restriction map for
pFN21AB7297
(Linear) (Six-base) MAPSORT of: KIBB7297.seq Check: 62 from: 1 to: 3678
KIBB7297.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 990 2209 2973 3678
Size: 990 1219 764 705
Fragments arranged by size:
1219 990 764 705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2438 3678
Size: 2438 1240
AflIII A'CryG_T
Cuts at: 0 609 3678
Size: 609 3069
AleI CACnn'nnGTG
Cuts at: 0 2669 3678
Size: 2669 1009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 802 1130 1910 2545 3521 3678
Size: 283 519 328 780 635 976 157
Fragments arranged by size:
976 780 635 519 328 283 157
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1969 2001 3678
Size: 1969 32 1677
Fragments arranged by size:
1969 1677 32
AlwNI CAG_nnn'CTG
Cuts at: 0 190 509 655 957 2045 2814 3043 3325
Size: 190 319 146 302 1088 769 229 282
Cuts at: 3325 3397 3604 3678
Size: 72 207 74
Fragments arranged by size:
1088 769 319 302 282 229 207 190
146 74 72
ApaI G_GGCC'C
Cuts at: 0 1097 1574 1790 1854 2411 2580 3307 3678
Size: 1097 477 216 64 557 169 727 371
Fragments arranged by size:
1097 727 557 477 371 216 169 64
ApaLI G'TGCA_C
Cuts at: 0 1595 1885 3678
Size: 1595 290 1793
Fragments arranged by size:
1793 1595 290
AvaI C'yCGr_G
Cuts at: 0 3013 3300 3559 3678
Size: 3013 287 259 119
Fragments arranged by size:
3013 287 259 119
BamHI G'GATC_C
Cuts at: 0 391 2606 3678
Size: 391 2215 1072
Fragments arranged by size:
2215 1072 391
BanI G'GyrC_C
Cuts at: 0 305 1284 1911 2221 2758 3141 3646 3678
Size: 305 979 627 310 537 383 505 32
Fragments arranged by size:
979 627 537 505 383 310 305 32
BanII G_rGCy'C
Cuts at: 0 1097 1346 1511 1574 1678 1790 1854 2196
Size: 1097 249 165 63 104 112 64 342
Cuts at: 2196 2411 2580 3194 3307 3471 3678
Size: 215 169 614 113 164 207
Fragments arranged by size:
1097 614 342 249 215 207 169 165
164 113 112 104 64 63
BbeI G_GCGC'C
Cuts at: 0 309 1288 3678
Size: 309 979 2390
Fragments arranged by size:
2390 979 309
BbsI GAAGACnn'nnnn_
Cuts at: 0 3207 3635 3678
Size: 3207 428 43
Fragments arranged by size:
3207 428 43
BbvCI CC'TCA_GC
Cuts at: 0 1182 3678
Size: 1182 2496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 988 1022 2891 2925 3678
Size: 988 34 1869 34 753
Fragments arranged by size:
1869 988 753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2798 3468 3678
Size: 2798 670 210
Fragments arranged by size:
2798 670 210
BclI T'GATC_A
Cuts at: 0 932 3678
Size: 932 2746
BglI GCCn_nnn'nGGC
Cuts at: 0 444 2434 3132 3678
Size: 444 1990 698 546
Fragments arranged by size:
1990 698 546 444
BlpI GC'TnA_GC
Cuts at: 0 1049 1345 3678
Size: 1049 296 2333
Fragments arranged by size:
2333 1049 296
Bme1580I G_kGCm'C
Cuts at: 0 128 1017 1097 1158 1574 1599 1790 1828
Size: 128 889 80 61 416 25 191 38
Cuts at: 1828 1854 1889 1916 2411 2580 2909 3307 3678
Size: 26 35 27 495 169 329 398 371
Fragments arranged by size:
889 495 416 398 371 329 191 169
128 80 61 38 35 27 26 25
BmgBI CAC'GTC
Cuts at: 0 1980 3678
Size: 1980 1698
BmrI ACTGGGnnnn_n'
Cuts at: 0 2913 2937 3678
Size: 2913 24 741
Fragments arranged by size:
2913 741 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 485 589 1015 1360 1692 2269 2377
Size: 222 263 104 426 345 332 577 108
Cuts at: 2377 2557 3548 3558 3644 3678
Size: 180 991 10 86 34
Fragments arranged by size:
991 577 426 345 332 263 222 180
108 104 86 34 10
Bpu10I CC'TnA_GC
Cuts at: 0 958 1182 1782 3678
Size: 958 224 600 1896
Fragments arranged by size:
1896 958 600 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2182 2380 2551 3678
Size: 2182 198 171 1127
Fragments arranged by size:
2182 1127 198 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 686 1198 1255 2636 3678
Size: 686 512 57 1381 1042
Fragments arranged by size:
1381 1042 686 512 57
BsaAI yAC'GTr
Cuts at: 0 165 1215 3678
Size: 165 1050 2463
Fragments arranged by size:
2463 1050 165
BsaHI Gr'CG_yC
Cuts at: 0 306 1285 1934 3124 3678
Size: 306 979 649 1190 554
Fragments arranged by size:
1190 979 649 554 306
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 988 1018 1345 1375 1738 1768
Size: 442 30 516 30 327 30 363 30
Cuts at: 1768 2077 2107 3073 3103 3678
Size: 309 30 966 30 575
Fragments arranged by size:
966 575 516 442 363 327 309 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1161 1170 1366 1834 2554 2615 2764 3678
Size: 1161 9 196 468 720 61 149 914
Fragments arranged by size:
1161 914 720 468 196 149 61 9
BseYI C'CCAG_C
Cuts at: 0 177 708 729 1010 1022 1039 1406 1758
Size: 177 531 21 281 12 17 367 352
Cuts at: 1758 1853 1907 2166 2214 2573 2784 2913 2928
Size: 95 54 259 48 359 211 129 15
Cuts at: 2928 3494 3678
Size: 566 184
Fragments arranged by size:
566 531 367 359 352 281 259 211
184 177 129 95 54 48 21 17
15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 253 334 652 859 1274 1315 1548
Size: 70 183 81 318 207 415 41 233
Cuts at: 1548 2227 2677 2812 3012 3180 3678
Size: 679 450 135 200 168 498
Fragments arranged by size:
679 498 450 415 318 233 207 200
183 168 135 81 70 41
BsiEI CG_ry'CG
Cuts at: 0 3240 3678
Size: 3240 438
BsiHKAI G_wGCw'C
Cuts at: 0 1055 1220 1346 1599 1678 1889 2196 2534
Size: 1055 165 126 253 79 211 307 338
Cuts at: 2534 2728 2950 3236 3471 3678
Size: 194 222 286 235 207
Fragments arranged by size:
1055 338 307 286 253 235 222 211
207 194 165 126 79
BsmI GAATG_Cn'
Cuts at: 0 485 1492 1899 3678
Size: 485 1007 407 1779
Fragments arranged by size:
1779 1007 485 407
Bsp1286I G_dGCh'C
Cuts at: 0 128 1017 1055 1097 1158 1220 1346 1511
Size: 128 889 38 42 61 62 126 165
Cuts at: 1511 1574 1599 1678 1790 1828 1854 1889 1916
Size: 63 25 79 112 38 26 35 27
Cuts at: 1916 2196 2411 2534 2580 2728 2909 2950 3194
Size: 280 215 123 46 148 181 41 244
Cuts at: 3194 3236 3307 3471 3678
Size: 42 71 164 207
Fragments arranged by size:
889 280 244 215 207 181 165 164
148 128 126 123 112 79 71 63
62 61 46 42 42 41 38 38
35 27 26 25
BspHI T'CATG_A
Cuts at: 0 860 1200 1988 3678
Size: 860 340 788 1690
Fragments arranged by size:
1690 860 788 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 674 713 990 1492 2209 2510 2973
Size: 38 636 39 277 502 717 301 463
Cuts at: 2973 3678
Size: 705
Fragments arranged by size:
717 705 636 502 463 301 277 39
38
BsrBI CCG'CTC
Cuts at: 0 573 744 3678
Size: 573 171 2934
Fragments arranged by size:
2934 573 171
BsrDI GCAATG_nn'
Cuts at: 0 2423 3018 3678
Size: 2423 595 660
Fragments arranged by size:
2423 660 595
BsrFI r'CCGG_y
Cuts at: 0 1207 2290 2630 2702 2850 2873 3266 3678
Size: 1207 1083 340 72 148 23 393 412
Fragments arranged by size:
1207 1083 412 393 340 148 72 23
BsrGI T'GTAC_A
Cuts at: 0 458 3678
Size: 458 3220
BssHII G'CGCG_C
Cuts at: 0 14 3678
Size: 14 3664
BstAPI GCAn_nnn'nTGC
Cuts at: 0 190 496 1610 3141 3397 3678
Size: 190 306 1114 1531 256 281
Fragments arranged by size:
1531 1114 306 281 256 190
BstEII G'GTnAC_C
Cuts at: 0 2286 3678
Size: 2286 1392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 708 1171 2355 2921 3442 3678
Size: 708 463 1184 566 521 236
Fragments arranged by size:
1184 708 566 521 463 236
BstYI r'GATC_y
Cuts at: 0 391 2210 2606 3678
Size: 391 1819 396 1072
Fragments arranged by size:
1819 1072 396 391
Bsu36I CC'TnA_GG
Cuts at: 0 1098 1144 2879 3605 3678
Size: 1098 46 1735 726 73
Fragments arranged by size:
1735 1098 726 73 46
BtgI C'CryG_G
Cuts at: 0 62 822 2348 3092 3678
Size: 62 760 1526 744 586
Fragments arranged by size:
1526 760 744 586 62
BtsI GCAGTG_nn'
Cuts at: 0 1456 1476 1623 1864 2142 3142 3337 3678
Size: 1456 20 147 241 278 1000 195 341
Fragments arranged by size:
1456 1000 341 278 241 195 147 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2577 2612 3678
Size: 2577 35 1066
Fragments arranged by size:
2577 1066 35
DraIII CAC_nnn'GTG
Cuts at: 0 168 1068 2655 3678
Size: 168 900 1587 1023
Fragments arranged by size:
1587 1023 900 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 239 2894 3211 3678
Size: 239 2655 317 467
Fragments arranged by size:
2655 467 317 239
EaeI y'GGCC_r
Cuts at: 0 1209 1656 2852 3444 3678
Size: 1209 447 1196 592 234
Fragments arranged by size:
1209 1196 592 447 234
EarI CTCTTCn'nnn_
Cuts at: 0 2677 3678
Size: 2677 1001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1981 2119 2689 3678
Size: 1981 138 570 989
Fragments arranged by size:
1981 989 570 138
EcoICRI GAG'CTC
Cuts at: 0 1344 1676 2194 3469 3678
Size: 1344 332 518 1275 209
Fragments arranged by size:
1344 1275 518 332 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 485 589 1015 1360 1692 2269 2377
Size: 222 263 104 426 345 332 577 108
Cuts at: 2377 2438 2557 3548 3558 3644 3678
Size: 61 119 991 10 86 34
Fragments arranged by size:
991 577 426 345 332 263 222 119
108 104 86 61 34 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 1294 3678
Size: 997 297 2384
Fragments arranged by size:
2384 997 297
EcoO109I rG'GnC_Cy
Cuts at: 0 281 663 1035 1094 1499 1570 1571 1786
Size: 281 382 372 59 405 71 1 215
Cuts at: 1786 1832 2031 2408 2499 2577 3304 3376 3384
Size: 46 199 377 91 78 727 72 8
Cuts at: 3384 3491 3678
Size: 107 187
Fragments arranged by size:
727 405 382 377 372 281 215 199
187 107 91 78 72 71 59 46
8 1
EcoRV GAT'ATC
Cuts at: 0 3179 3678
Size: 3179 499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1629 3678
Size: 1629 2049
HaeII r_GCGC'y
Cuts at: 0 309 1032 1288 2566 2709 3678
Size: 309 723 256 1278 143 969
Fragments arranged by size:
1278 969 723 309 256 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 1162 1194 2077 2109 2591 2623
Size: 140 32 990 32 883 32 482 32
Cuts at: 2623 2954 2986 3466 3483 3498 3515 3678
Size: 331 32 480 17 15 17 163
Fragments arranged by size:
990 883 482 480 331 163 140 32
32 32 32 32 17 17 15
KasI G'GCGC_C
Cuts at: 0 305 1284 3678
Size: 305 979 2394
Fragments arranged by size:
2394 979 305
MscI TGG'CCA
Cuts at: 0 1658 3678
Size: 1658 2020
MslI CAynn'nnrTG
Cuts at: 0 205 478 784 859 1129 1169 1892 2272
Size: 205 273 306 75 270 40 723 380
Cuts at: 2272 2347 2669 3440 3678
Size: 75 322 771 238
Fragments arranged by size:
771 723 380 322 306 273 270 238
205 75 75 40
MspA1I CmG'CkG
Cuts at: 0 64 493 599 729 1328 1521 1578 1907
Size: 64 429 106 130 599 193 57 329
Cuts at: 1907 2405 2979 3678
Size: 498 574 699
Fragments arranged by size:
699 599 574 498 429 329 193 130
106 64 57
NaeI GCC'GGC
Cuts at: 0 2632 2704 2852 3678
Size: 2632 72 148 826
Fragments arranged by size:
2632 826 148 72
NarI GG'CG_CC
Cuts at: 0 306 1285 3678
Size: 306 979 2393
Fragments arranged by size:
2393 979 306
NcoI C'CATG_G
Cuts at: 0 822 2348 3678
Size: 822 1526 1330
Fragments arranged by size:
1526 1330 822
NdeI CA'TA_TG
Cuts at: 0 1653 3678
Size: 1653 2025
NgoMIV G'CCGG_C
Cuts at: 0 2630 2702 2850 3678
Size: 2630 72 148 828
Fragments arranged by size:
2630 828 148 72
NspI r_CATG'y
Cuts at: 0 613 760 838 1134 3678
Size: 613 147 78 296 2544
Fragments arranged by size:
2544 613 296 147 78
PasI CC'CwG_GG
Cuts at: 0 897 1828 3487 3678
Size: 897 931 1659 191
Fragments arranged by size:
1659 931 897 191
PciI A'CATG_T
Cuts at: 0 609 3678
Size: 609 3069
PflMI CCAn_nnn'nTGG
Cuts at: 0 1765 2652 3325 3526 3678
Size: 1765 887 673 201 152
Fragments arranged by size:
1765 887 673 201 152
PfoI T'CCnGG_A
Cuts at: 0 1678 2534 3357 3408 3535 3678
Size: 1678 856 823 51 127 143
Fragments arranged by size:
1678 856 823 143 127 51
PmlI CAC'GTG
Cuts at: 0 165 1215 3678
Size: 165 1050 2463
Fragments arranged by size:
2463 1050 165
PpuMI rG'GwC_Cy
Cuts at: 0 281 663 1035 1499 1832 2031 2499 3376
Size: 281 382 372 464 333 199 468 877
Cuts at: 3376 3384 3491 3678
Size: 8 107 187
Fragments arranged by size:
877 468 464 382 372 333 281 199
187 107 8
PspOMI G'GGCC_C
Cuts at: 0 1093 1570 1786 1850 2407 2576 3303 3678
Size: 1093 477 216 64 557 169 727 375
Fragments arranged by size:
1093 727 557 477 375 216 169 64
PstI C_TGCA'G
Cuts at: 0 133 195 1333 2023 2149 2587 2767 3058
Size: 133 62 1138 690 126 438 180 291
Cuts at: 3058 3393 3402 3678
Size: 335 9 276
Fragments arranged by size:
1138 690 438 335 291 276 180 133
126 62 9
PvuI CG_AT'CG
Cuts at: 0 3240 3678
Size: 3240 438
PvuII CAG'CTG
Cuts at: 0 493 729 1521 1907 3678
Size: 493 236 792 386 1771
Fragments arranged by size:
1771 792 493 386 236
SacI G_AGCT'C
Cuts at: 0 1346 1678 2196 3471 3678
Size: 1346 332 518 1275 207
Fragments arranged by size:
1346 1275 518 332 207
SacII CC_GC'GG
Cuts at: 0 65 3678
Size: 65 3613
SanDI GG'GwC_CC
Cuts at: 0 281 2031 3491 3678
Size: 281 1750 1460 187
Fragments arranged by size:
1750 1460 281 187
SexAI A'CCwGG_T
Cuts at: 0 1802 1994 2282 3678
Size: 1802 192 288 1396
Fragments arranged by size:
1802 1396 288 192
SfcI C'TryA_G
Cuts at: 0 129 191 774 1329 2019 2145 2583 2763
Size: 129 62 583 555 690 126 438 180
Cuts at: 2763 3054 3389 3398 3678
Size: 291 335 9 280
Fragments arranged by size:
690 583 555 438 335 291 280 180
129 126 62 9
SfoI GGC'GCC
Cuts at: 0 307 1286 3678
Size: 307 979 2392
Fragments arranged by size:
2392 979 307
SgrAI Cr'CCGG_yG
Cuts at: 0 2702 3678
Size: 2702 976
SmaI CCC'GGG
Cuts at: 0 3561 3678
Size: 3561 117
SmlI C'TyrA_G
Cuts at: 0 2197 2395 2566 3678
Size: 2197 198 171 1112
Fragments arranged by size:
2197 1112 198 171
SphI G_CATG'C
Cuts at: 0 760 3678
Size: 760 2918
SspI AAT'ATT
Cuts at: 0 915 3678
Size: 915 2763
StuI AGG'CCT
Cuts at: 0 2176 2593 3298 3678
Size: 2176 417 705 380
Fragments arranged by size:
2176 705 417 380
StyI C'CwwG_G
Cuts at: 0 822 2312 2348 2384 3571 3678
Size: 822 1490 36 36 1187 107
Fragments arranged by size:
1490 1187 822 107 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 287 1899 2730 3129 3678
Size: 234 53 1612 831 399 549
Fragments arranged by size:
1612 831 549 399 234 53
TatI w'GTAC_w
Cuts at: 0 458 3678
Size: 458 3220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 877 909 988 1020 1236 1268
Size: 383 32 462 32 79 32 216 32
Cuts at: 1268 1435 1467 1578 1610 2098 2130 3073 3105
Size: 167 32 111 32 488 32 943 32
Cuts at: 3105 3678
Size: 573
Fragments arranged by size:
943 573 488 462 383 216 167 111
79 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3043 3678
Size: 3043 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 169 375 2430 3134 3678
Size: 169 206 2055 704 544
Fragments arranged by size:
2055 704 544 206 169
XmaI C'CCGG_G
Cuts at: 0 3559 3678
Size: 3559 119
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuI PvuII SacI SacII
SanDI SexAI SfcI SfoI SgrAI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
ApoI AscI AseI AsiSI AvrII BaeI BfrBI BglII
BmtI BplI BsaBI BsaWI BsiWI BsmBI BspEI BssSI
BstBI BstZ17I BtgZI ClaI DraI EagI EcoRI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspXI PsrI RsrII SalI SapI SbfI
ScaI SfiI SgfI SnaBI SpeI SrfI SwaI XbaI
XhoI XmnI ZraI