Restriction map for
pFN21AB7294
(Circular) (Six-base) MAPSORT of: pFN21AB7294.seq Check: 3930 from: 1 to: 7012
pFN21AB7294 7012 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 3401 4040 2465
Size: 936 639 5437
Fragments arranged by size:
5437 936 639
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4822 278
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2946 4355 1937
Size: 1009 1409 4594
Fragments arranged by size:
4594 1409 1009
Acc65I G'GTAC_C
Cuts at: 4333 4333
Size: 7012
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2338 4009 5075 6459 6727 1632
Size: 706 1671 1066 1384 268 1917
Fragments arranged by size:
1917 1671 1384 1066 706 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6815
AflIII A'CryG_T
Cuts at: 1203 3978 5911 6888 1203
Size: 2775 1933 977 1327
Fragments arranged by size:
2775 1933 1327 977
AgeI A'CCGG_T
Cuts at: 5843 5843
Size: 7012
AhdI GACnn_n'nnGTC
Cuts at: 2079 5741 2079
Size: 3662 3350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3193 1184
Size: 2009 5003
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 3209 3241 2420
Size: 32 757 32 6191
Fragments arranged by size:
6191 757 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2203 2467 3124 3136 3280 4013 4032 4755 6327
Size: 264 657 12 144 733 19 723 1572
Cuts at: 6327 2203
Size: 2888
Fragments arranged by size:
2888 1572 733 723 657 264 144 19
12
ApaI G_GGCC'C
Cuts at: 2240 2240
Size: 7012
ApaLI G'TGCA_C
Cuts at: 2705 5069 6225 2705
Size: 2364 1156 3492
Fragments arranged by size:
3492 2364 1156
ApoI r'AATT_y
Cuts at: 1493 4321 4547 4699 6808 1493
Size: 2828 226 152 2109 1697
Fragments arranged by size:
2828 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 2995 3651 4337 1870
Size: 73 1052 656 686 4545
Fragments arranged by size:
4545 1052 686 656 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4117 4150 4117
Size: 33 6979
BamHI G'GATC_C
Cuts at: 4342 4709 4342
Size: 367 6645
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4199 4333 5788 618
Size: 325 217 627 2412 134 1455 1842
Fragments arranged by size:
2412 1842 1455 627 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2095 2137 2177 2240 2514 2542 2973 3167
Size: 704 42 40 63 274 28 431 194
Cuts at: 3167 3471 3832 4331 1391
Size: 304 361 499 4072
Fragments arranged by size:
4072 704 499 431 361 304 274 194
63 42 40 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 928
Size: 971 571 5470
Fragments arranged by size:
5470 971 571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5203 5237 1903
Size: 34 3266 34 3678
Fragments arranged by size:
3678 3266 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 4904 6114 4145
Size: 759 1210 5043
Fragments arranged by size:
5043 1210 759
BclI T'GATC_A
Cuts at: 1548 2739 1548
Size: 1191 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2642 5623 136
Size: 107 122 71 631 605 970 2981 1525
Fragments arranged by size:
2981 1525 970 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7007 7007
Size: 7012
BlpI GC'TnA_GC
Cuts at: 4429 4429
Size: 7012
Bme1580I G_kGCm'C
Cuts at: 1790 2240 2709 3575 5073 6229 1790
Size: 450 469 866 1498 1156 2573
Fragments arranged by size:
2573 1498 1156 866 469 450
BmgBI CAC'GTC
Cuts at: 1318 3790 4155 1318
Size: 2472 365 4175
Fragments arranged by size:
4175 2472 365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2632 2967 3231
Size: 416 53 83 399 24 1215 335 264
Cuts at: 3231 4743 5701 442
Size: 1512 958 1753
Fragments arranged by size:
1753 1512 1215 958 416 399 335 264
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2327 2412 2942 3846 5672 1239
Size: 870 218 85 530 904 1826 2579
Fragments arranged by size:
2579 1826 904 870 530 218 85
Bpu10I CC'TnA_GC
Cuts at: 2967 3941 2967
Size: 974 6038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3305 5137 6002 6300 3305
Size: 1832 865 298 4017
Fragments arranged by size:
4017 1832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2388 2918 3539 5675 882
Size: 617 889 530 621 2136 2219
Fragments arranged by size:
2219 2136 889 621 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 2341 3037 3916 4803 493
Size: 711 1137 696 879 887 2702
Fragments arranged by size:
2702 1137 887 879 711 696
BsaBI GATnn'nnATC
Cuts at: 7006 7006
Size: 7012
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3450 4819 5201 5836
Size: 53 83 186 936 1917 1369 382 635
Cuts at: 5836 275
Size: 1451
Fragments arranged by size:
1917 1451 1369 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2571 3159 4301 5445 5843 6117 6264 6675 2571
Size: 588 1142 1144 398 274 147 411 2908
Fragments arranged by size:
2908 1144 1142 588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 2420 2450 3900
Size: 30 165 30 909 30 78 30 1450
Cuts at: 3900 3930 4296 4326 1178
Size: 30 366 30 3864
Fragments arranged by size:
3864 1450 909 366 165 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2320 2573 2637 3386 3797 3822 1186
Size: 1129 5 253 64 749 411 25 4376
Fragments arranged by size:
4376 1129 749 411 253 64 25 5
BseYI C'CCAG_C
Cuts at: 2022 2115 2119 2153 2275 2373 3124 3199 3765
Size: 93 4 34 122 98 751 75 566
Cuts at: 3765 4193 4226 4269 6215 6711 2022
Size: 428 33 43 1946 496 2323
Fragments arranged by size:
2323 1946 751 566 496 428 122 98
93 75 43 34 33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 3413 3421 3478 3535 3569 3796
Size: 573 1191 486 8 57 57 34 227
Cuts at: 3796 3938 1163
Size: 142 4237
Fragments arranged by size:
4237 1191 573 486 227 142 57 57
34 8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2660 4462 5223 6251 1540
Size: 275 186 659 1802 761 1028 2301
Fragments arranged by size:
2301 1802 1028 761 659 275 186
BsiHKAI G_wGCw'C
Cuts at: 2095 2177 2709 3017 3167 3832 5073 5158 6229
Size: 82 532 308 150 665 1241 85 1071
Cuts at: 6229 2095
Size: 2878
Fragments arranged by size:
2878 1241 1071 665 532 308 150 85
82
BsmI GAATG_Cn'
Cuts at: 4523 4616 4523
Size: 93 6919
BsmBI CGTCTCn'nnnn_
Cuts at: 4166 4166
Size: 7012
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2137 2177 2240 2514 2542 2709
Size: 399 305 42 40 63 274 28 167
Cuts at: 2709 2973 3017 3167 3471 3575 3832 4331 5073
Size: 264 44 150 304 104 257 499 742
Cuts at: 5073 5158 6229 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 305 304 274
264 257 167 150 104 85 63 44
42 40 28
BspEI T'CCGG_A
Cuts at: 4301 6675 4301
Size: 2374 4638
BspHI T'CATG_A
Cuts at: 4901 6631 4901
Size: 1730 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2465 2671 3240 3401 3557 3591 4040 4367
Size: 1621 206 569 161 156 34 449 327
Cuts at: 4367 844
Size: 3489
Fragments arranged by size:
3489 1621 569 449 327 206 161 156
34
BsrBI CCG'CTC
Cuts at: 1394 1823 4899 1394
Size: 429 3076 3507
Fragments arranged by size:
3507 3076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4090 4221 5507 5681 65
Size: 1183 2842 131 1286 174 1396
Fragments arranged by size:
2842 1396 1286 1183 174 131
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 3657 5656 5843 1700
Size: 110 845 1002 1999 187 2869
Fragments arranged by size:
2869 1999 1002 845 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7012
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 3016 5072 6084 3016
Size: 2056 1012 3944
Fragments arranged by size:
3944 2056 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3423 3432 3790 3966 4128 794
Size: 2629 9 358 176 162 3678
Fragments arranged by size:
3678 2629 358 176 162 9
BstBI TT'CG_AA
Cuts at: 5816 5816
Size: 7012
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7012
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2725 2809 3195 4255 2161
Size: 564 84 386 1060 4918
Fragments arranged by size:
4918 1060 564 386 84
BstYI r'GATC_y
Cuts at: 1972 2590 2898 3758 4342 4709 5094 5111 6563
Size: 618 308 860 584 367 385 17 1452
Cuts at: 6563 6649 6661 7007 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 860 618 584 385 367 346
308 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2139 2544 2139
Size: 405 6607
BtgI C'CryG_G
Cuts at: 513 1065 2808 2871 3061 3497 4069 4164 513
Size: 552 1743 63 190 436 572 95 3361
Fragments arranged by size:
3361 1743 572 552 436 190 95 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2113 3113 3523 3980 487
Size: 683 56 887 1000 410 457 3519
Fragments arranged by size:
3519 1000 887 683 457 410 56
BtsI GCAGTG_nn'
Cuts at: 2398 3668 4048 4528 5322 5342 2398
Size: 1270 380 480 794 20 4068
Fragments arranged by size:
4068 1270 794 480 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7012
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6977
DraI TTT'AAA
Cuts at: 4279 4316 4279
Size: 37 6975
DraIII CAC_nnn'GTG
Cuts at: 3503 3503
Size: 7012
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2213 2958 6019 809
Size: 507 897 745 3061 1802
Fragments arranged by size:
3061 1802 897 745 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2657 2820 3102
Size: 54 1425 50 275 18 827 163 282
Cuts at: 3102 4459 5348 6977 8
Size: 1357 889 1629 43
Fragments arranged by size:
1629 1425 1357 889 827 282 275 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2657 4459 1537
Size: 275 845 1802 4090
Fragments arranged by size:
4090 1802 845 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2996 3311 4942 1353
Size: 1643 315 1631 3423
Fragments arranged by size:
3423 1643 1631 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2207 2675 3941 5599 5973 6119
Size: 1064 138 618 468 1266 1658 374 146
Cuts at: 6119 387
Size: 1280
Fragments arranged by size:
1658 1280 1266 1064 618 468 374 146
138
EcoICRI GAG'CTC
Cuts at: 2093 2175 3165 3830 2093
Size: 82 990 665 5275
Fragments arranged by size:
5275 990 665 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2327 2338 2412 2942 3846 4009
Size: 393 477 218 11 74 530 904 163
Cuts at: 4009 5075 5672 6459 6727 1239
Size: 1066 597 787 268 1524
Fragments arranged by size:
1524 1066 904 787 597 530 477 393
268 218 163 74 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3837 3837
Size: 7012
EcoO109I rG'GnC_Cy
Cuts at: 2073 2081 2236 2549 3003 3844 4190 2073
Size: 8 155 313 454 841 346 4895
Fragments arranged by size:
4895 841 454 346 313 155 8
EcoRI G'AATT_C
Cuts at: 4321 4699 4321
Size: 378 6634
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 2023 2350 4054 6159 1678
Size: 345 327 1704 2105 2531
Fragments arranged by size:
2531 2105 1704 345 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 3371 3403 5653
Size: 32 135 32 493 32 1274 32 2250
Cuts at: 5653 5685 5727 5759 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2250 1274 493 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3817 4356 4602 669
Size: 988 281 1879 539 246 3079
Fragments arranged by size:
3079 1879 988 539 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7012
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KpnI G_GTAC'C
Cuts at: 4337 4337
Size: 7012
MfeI C'AATT_G
Cuts at: 4611 4611
Size: 7012
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6126 6310 1305
Size: 4821 184 2007
Fragments arranged by size:
4821 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5190
Fragments arranged by size:
5190 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4970 5329 5488 518
Size: 648 3804 359 159 2042
Fragments arranged by size:
3804 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2203 2755 3024 3300 3483 3627 3922
Size: 513 84 552 269 276 183 144 295
Cuts at: 3922 4197 4428 5105 6253 6498 1606
Size: 275 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 513 295 276 275
269 245 231 183 144 84
NcoI C'CATG_G
Cuts at: 513 1065 2871 513
Size: 552 1806 4654
Fragments arranged by size:
4654 1806 552
NdeI CA'TA_TG
Cuts at: 387 3047 387
Size: 2660 4352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NspI r_CATG'y
Cuts at: 1132 1229 3982 4370 5915 1132
Size: 97 2753 388 1545 2229
Fragments arranged by size:
2753 2229 1545 388 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 3978 5911 3978
Size: 1933 5079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 3124 1108
Size: 1413 603 4996
Fragments arranged by size:
4996 1413 603
PfoI T'CCnGG_A
Cuts at: 2919 3327 2919
Size: 408 6604
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PmlI CAC'GTG
Cuts at: 3916 3916
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2420 2452 5081 5113 6594 6626 2420
Size: 32 2629 32 1481 32 2806
Fragments arranged by size:
2806 2629 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2073 2081 2549 3003 3844 4190 2073
Size: 8 468 454 841 346 4895
Fragments arranged by size:
4895 841 468 454 346 8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7012
PsiI TTA'TAA
Cuts at: 4582 4582
Size: 7012
PspOMI G'GGCC_C
Cuts at: 2236 2236
Size: 7012
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2483 2515 2483
Size: 32 6980
PstI C_TGCA'G
Cuts at: 830 3285 3905 4082 4133 4364 830
Size: 2455 620 177 51 231 3478
Fragments arranged by size:
3478 2455 620 231 177 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
PvuII CAG'CTG
Cuts at: 2119 2203 2755 3300 3922 2119
Size: 84 552 545 622 5209
Fragments arranged by size:
5209 622 552 545 84
SacI G_AGCT'C
Cuts at: 2095 2177 3167 3832 2095
Size: 82 990 665 5275
Fragments arranged by size:
5275 990 665 82
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SanDI GG'GwC_CC
Cuts at: 2073 2549 2073
Size: 476 6536
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 5260 6972 5260
Size: 1712 5300
SexAI A'CCwGG_T
Cuts at: 3846 3846
Size: 7012
SfcI C'TryA_G
Cuts at: 826 1045 2700 3253 3281 3901 4078 4129 4360
Size: 219 1655 553 28 620 177 51 231
Cuts at: 4360 5495 6176 6367 826
Size: 1135 681 191 1471
Fragments arranged by size:
1655 1471 1135 681 620 553 231 219
191 177 51 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 3653 4339 1872
Size: 1781 686 4545
Fragments arranged by size:
4545 1781 686
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3320 5116 6017 6279 820
Size: 197 926 1377 1796 901 262 1553
Fragments arranged by size:
1796 1553 1377 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4803 493
Size: 4310 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7012
SphI G_CATG'C
Cuts at: 1132 4370 1132
Size: 3238 3774
SspI AAT'ATT
Cuts at: 5 52 4936 5
Size: 47 4884 2081
Fragments arranged by size:
4884 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7012
StyI C'CwwG_G
Cuts at: 513 1065 2077 2531 2871 4451 513
Size: 552 1012 454 340 1580 3074
Fragments arranged by size:
3074 1580 1012 552 454 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2999 4177 5039 5056 5209 5394 5839
Size: 1255 1419 1178 862 17 153 185 445
Cuts at: 5839 325
Size: 1498
Fragments arranged by size:
1498 1419 1255 1178 862 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3267 5258 6970
Size: 274 80 33 51 1443 1290 1991 1712
Cuts at: 6970 96
Size: 138
Fragments arranged by size:
1991 1712 1443 1290 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 3167 3199 3647 3679 3721 3753 3779
Size: 32 823 32 448 32 42 32 26
Cuts at: 3779 3811 3900 3932 6972 7004 2312
Size: 32 89 32 3040 32 2320
Fragments arranged by size:
3040 2320 823 448 89 42 32 32
32 32 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7012
XbaI T'CTAG_A
Cuts at: 4348 4348
Size: 7012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2084 2468 2522 2084
Size: 384 54 6574
Fragments arranged by size:
6574 384 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 3651 4337 1870
Size: 1781 686 4545
Fragments arranged by size:
4545 1781 686
XmnI GAAnn'nnTTC
Cuts at: 3353 5141 3353
Size: 1788 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4820 276
Size: 53 83 186 936 3286 2468
Fragments arranged by size:
3286 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI RsrII SacII SapI
SfiI SfoI SrfI SwaI