Restriction map for
pFN21AB7293
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BbeI BciVI
BfaI BfrI BlnI BsaWI BseAI
BsiWI BsmI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MunI NarI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1359 CGCG CG^CG
AflIII 2342 ACGTGT A^C[GA][CT]GT
AhdI 3032 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1287 GTGCAC G^TGCAC
ApaLI 1287 GTGCAC G^TGCAC
AspEI 3032 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3012 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 158 TGCGCA TGC^GCA
BbrPI 2026 CACGTG CAC^GTG
BcgI 1013 GCACCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1367 TGATCA T^GATCA
BglII 2846 AGATCT A^GATCT
BsaAI 2026 CACGTG [CT]AC^GT[GA]
BsaBI 516 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 179 CGATCG CG[GA][CT]^CG
Bsp1407I 2993 TGTACA T^GTACA
BspDI 181 ATCGAT AT^CGAT
BspHI 2318 TCATGA T^CATGA
BsrFI 320 GCCGGC [GA]^CCGG[CT]
BsrGI 2993 TGTACA T^GTACA
BssSI 331 CACGAG CACGAG(-5/-1)
BstUI 1359 CGCG CG^CG
Cfr10I 320 GCCGGC [GA]^CCGG[CT]
ClaI 181 ATCGAT AT^CGAT
DrdI 967 GACATTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3032 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1789 CTCTTC CTCTTC(1/4)
FbaI 1367 TGATCA T^GATCA
FspI 158 TGCGCA TGC^GCA
HincII 2815 GTCAAC GT[CT]^[GA]AC
HindII 2815 GTCAAC GT[CT]^[GA]AC
Ksp632I 1789 CTCTTC CTCTTC(1/4)
MamI 516 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1359 CGCG CG^CG
NaeI 320 GCCGGC GCC^GGC
NdeI 197 CATATG CA^TATG
NgoMIV 320 GCCGGC G^CCGGC
PflFI 3012 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2026 CACGTG CAC^GTG
PmlI 2026 CACGTG CAC^GTG
PstI 1260 CTGCAG CTGCA^G
PvuI 179 CGATCG CGAT^CG
RcaI 2318 TCATGA T^CATGA
SapI 1788 GCTCTTC GCTCTTC(1/4)
SexAI 1753 ACCTGGT A^CC[AT]GGT
SspBI 2993 TGTACA T^GTACA
TaqI 182 TCGA T^CGA
Tsp509I 1831 AATT ^AATT
Tth111I 3012 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 182 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
ApaI 633 GGGCCC GGGCC^C
1948 GGGCCC
BbvCI 1872 CCTCAGC CCTCAGC(-5/-2)
2004 CCTCAGC
BglI 990 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1307 GCCACTACGGC
BsgI 265 CTGCAC GTGCAG(16/14)
45 GTGCAG
BsmBI 22 GAGACG CGTCTC(1/5)
2930 GAGACG
BsrDI 786 CATTGC GCAATG(2/0)
2136 CATTGC
BstAPI 146 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2049 GCAGAGGATGC
BstEII 540 GGTGACC G^GT[ACGT]ACC
1084 GGTGACC
BstPI 540 GGTGACC G^GT[ACGT]ACC
1084 GGTGACC
EcoO65I 540 GGTGACC G^GT[ACGT]ACC
1084 GGTGACC
SacI 1570 GAGCTC GAGCT^C
2062 GAGCTC
SfcI 1103 CTATAG C^T[GA][CT]AG
1260 CTGCAG
SmlI 277 CTCAAG C^T[CT][GA]AG
1090 CTCAAG
TfiI 429 GATTC G^A[AT]TC
2693 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SphI
StuI StyI Tru9I TseI Tsp45I
TspRI Van91I XcmI XhoII