(Circular) (Six-base) MAPSORT of: pFN21AB7293.seq Check: 8373 from: 1 to: 7795 pFN21AB7293 7795 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2310 4888 2310 Size: 2578 5217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5605 278 Size: 53 83 186 936 4069 2468 Fragments arranged by size: 4069 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5138 1937 Size: 3201 4594 Acc65I G'GTAC_C Cuts at: 5116 5116 Size: 7795 AclI AA'CG_TT Cuts at: 1563 5922 6295 1563 Size: 4359 373 3063 Fragments arranged by size: 4359 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2941 3622 5858 7242 7510 1632 Size: 1309 681 2236 1384 268 1917 Fragments arranged by size: 2236 1917 1384 1309 681 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7598 AflIII A'CryG_T Cuts at: 1203 4347 6694 7671 1203 Size: 3144 2347 977 1327 Fragments arranged by size: 3144 2347 1327 977 AgeI A'CCGG_T Cuts at: 6626 6626 Size: 7795 AhdI GACnn_n'nnGTC Cuts at: 5042 6524 5042 Size: 1482 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7795 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2318 1184 Size: 1134 6661 AlwNI CAG_nnn'CTG Cuts at: 2866 3940 4442 5538 7110 2866 Size: 1074 502 1096 1572 3551 Fragments arranged by size: 3551 1572 1096 1074 502 ApaI G_GGCC'C Cuts at: 2642 3957 2642 Size: 1315 6480 ApaLI G'TGCA_C Cuts at: 3292 5852 7008 3292 Size: 2560 1156 4079 Fragments arranged by size: 4079 2560 1156 ApoI r'AATT_y Cuts at: 1493 5104 5330 5482 7591 1493 Size: 3611 226 152 2109 1697 Fragments arranged by size: 3611 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6349 160 Size: 872 5317 1606 Fragments arranged by size: 5317 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7795 AvaI C'yCGr_G Cuts at: 1870 1943 2393 4193 4740 4932 5120 1870 Size: 73 450 1800 547 192 188 4545 Fragments arranged by size: 4545 1800 547 450 192 188 73 BamHI G'GATC_C Cuts at: 5125 5492 5125 Size: 367 7428 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 2372 2609 2755 2992 Size: 325 217 627 407 178 237 146 237 Cuts at: 2992 3017 3245 3992 4424 5116 6571 618 Size: 25 228 747 432 692 1455 1842 Fragments arranged by size: 1842 1455 747 692 627 432 407 325 237 237 228 217 178 146 25 BanII G_rGCy'C Cuts at: 1391 2097 2241 2642 2657 3519 3579 3845 3957 Size: 706 144 401 15 862 60 266 112 Cuts at: 3957 4071 5114 1391 Size: 114 1043 4072 Fragments arranged by size: 4072 1043 862 706 401 266 144 114 112 60 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3433 3915 4275 4523 928 Size: 971 1534 482 360 248 4200 Fragments arranged by size: 4200 1534 971 482 360 248 BbvCI CC'TCA_GC Cuts at: 3878 4010 3878 Size: 132 7663 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3007 3041 5986 6020 1903 Size: 34 1070 34 2945 34 3678 Fragments arranged by size: 3678 2945 1070 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5687 6897 5687 Size: 1210 6585 BclI T'GATC_A Cuts at: 1548 3372 1548 Size: 1824 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3001 3318 6406 Size: 107 122 71 631 605 1329 317 3088 Cuts at: 6406 136 Size: 1525 Fragments arranged by size: 3088 1525 1329 631 605 317 122 107 71 BglII A'GATC_T Cuts at: 4851 7790 4851 Size: 2939 4856 BlpI GC'TnA_GC Cuts at: 2081 3573 4679 4746 5212 2081 Size: 1492 1106 67 466 4664 Fragments arranged by size: 4664 1492 1106 466 67 Bme1580I G_kGCm'C Cuts at: 1790 2199 2642 2758 3296 3957 3995 4092 4988 Size: 409 443 116 538 661 38 97 896 Cuts at: 4988 5856 7012 1790 Size: 868 1156 2573 Fragments arranged by size: 2573 1156 896 868 661 538 443 409 116 97 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3928 4703 5526 Size: 416 53 83 399 24 2511 775 823 Cuts at: 5526 6484 442 Size: 958 1753 Fragments arranged by size: 2511 1753 958 823 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2726 4055 5025 5033 6455 1239 Size: 1487 1329 970 8 1422 2579 Fragments arranged by size: 2579 1487 1422 1329 970 8 Bpu10I CC'TnA_GC Cuts at: 3878 4010 4065 3878 Size: 132 55 7608 Fragments arranged by size: 7608 132 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 3080 5920 6785 7083 2267 Size: 813 2840 865 298 2979 Fragments arranged by size: 2979 2840 865 813 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2717 2806 2893 3673 4196 5009 6458 Size: 617 1218 89 87 780 523 813 1449 Cuts at: 6458 882 Size: 2219 Fragments arranged by size: 2219 1449 1218 813 780 617 523 89 87 BsaAI yAC'GTr Cuts at: 493 1204 4033 5586 493 Size: 711 2829 1553 2702 Fragments arranged by size: 2829 2702 1553 711 BsaBI GATnn'nnATC Cuts at: 2525 7789 2525 Size: 5264 2531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2040 4164 5602 Size: 53 83 186 936 497 10 2124 1438 Cuts at: 5602 5984 6619 275 Size: 382 635 1451 Fragments arranged by size: 2124 1451 1438 936 635 497 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 6228 6626 6900 7047 7458 6228 Size: 398 274 147 411 6565 Fragments arranged by size: 6565 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2834 2864 2927 Size: 30 165 30 855 30 546 30 63 Cuts at: 2927 2957 5006 5036 1178 Size: 30 2049 30 3937 Fragments arranged by size: 3937 2049 855 546 165 63 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 3394 3743 3835 4490 4553 4606 4877 Size: 1225 983 349 92 655 63 53 271 Cuts at: 4877 1186 Size: 4104 Fragments arranged by size: 4104 1225 983 655 349 271 92 63 53 BseYI C'CCAG_C Cuts at: 2129 2226 2330 2415 3240 3297 4390 4988 6998 Size: 97 104 85 825 57 1093 598 2010 Cuts at: 6998 7494 2129 Size: 496 2430 Fragments arranged by size: 2430 2010 1093 825 598 496 104 97 85 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2255 1163 Size: 573 335 184 6703 Fragments arranged by size: 6703 573 335 184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2187 5245 6006 7034 1540 Size: 275 186 186 3058 761 1028 2301 Fragments arranged by size: 3058 2301 1028 761 275 186 186 BsiHKAI G_wGCw'C Cuts at: 2211 3296 3579 3795 3948 4038 4071 4752 5079 Size: 1085 283 216 153 90 33 681 327 Cuts at: 5079 5856 5941 7012 2211 Size: 777 85 1071 2994 Fragments arranged by size: 2994 1085 1071 777 681 327 283 216 153 90 85 33 BsmI GAATG_Cn' Cuts at: 5306 5399 5306 Size: 93 7702 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 4929 2021 Size: 2908 4887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2199 2211 2241 2642 2657 2758 Size: 399 307 102 12 30 401 15 101 Cuts at: 2758 3296 3519 3579 3795 3845 3948 3957 3995 Size: 538 223 60 216 50 103 9 38 Cuts at: 3995 4038 4071 4092 4752 4988 5079 5114 5856 Size: 43 33 21 660 236 91 35 742 Cuts at: 5856 5941 7012 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 660 538 401 399 307 236 223 216 103 102 101 91 85 60 50 43 38 35 33 30 21 15 12 9 BspEI T'CCGG_A Cuts at: 7458 7458 Size: 7795 BspHI T'CATG_A Cuts at: 4323 5684 7414 4323 Size: 1361 1730 4704 Fragments arranged by size: 4704 1730 1361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 2310 2481 2745 3540 4178 4888 5150 Size: 1433 33 171 264 795 638 710 262 Cuts at: 5150 844 Size: 3489 Fragments arranged by size: 3489 1433 795 710 638 264 262 171 33 BsrBI CCG'CTC Cuts at: 1394 1823 5682 1394 Size: 429 3859 3507 Fragments arranged by size: 3859 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2790 4140 6290 6464 65 Size: 1183 1542 1350 2150 174 1396 Fragments arranged by size: 2150 1542 1396 1350 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2325 6439 6626 1700 Size: 110 515 4114 187 2869 Fragments arranged by size: 4114 2869 515 187 110 BsrGI T'GTAC_A Cuts at: 96 4998 96 Size: 4902 2893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7795 BssSI C'ACGA_G Cuts at: 2336 5855 6867 2336 Size: 3519 1012 3264 Fragments arranged by size: 3519 3264 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2157 4060 794 Size: 1363 1903 4529 Fragments arranged by size: 4529 1903 1363 BstBI TT'CG_AA Cuts at: 6599 6599 Size: 7795 BstEII G'GTnAC_C Cuts at: 1184 2545 3089 1184 Size: 1361 544 5890 Fragments arranged by size: 5890 1361 544 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2750 2830 3349 3553 3787 4216 4465 2689 Size: 61 80 519 204 234 429 249 6019 Fragments arranged by size: 6019 519 429 249 234 204 80 61 BstYI r'GATC_y Cuts at: 1972 4170 4611 4851 5125 5492 5877 5894 7346 Size: 2198 441 240 274 367 385 17 1452 Cuts at: 7346 7432 7444 7790 1972 Size: 86 12 346 1977 Fragments arranged by size: 2198 1977 1452 441 385 367 346 274 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2688 2796 3735 513 Size: 552 939 684 108 939 4573 Fragments arranged by size: 4573 939 939 684 552 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3532 3553 3760 4063 487 Size: 683 56 2306 21 207 303 4219 Fragments arranged by size: 4219 2306 683 303 207 56 21 BtsI GCAGTG_nn' Cuts at: 2075 2092 5311 6105 6125 2075 Size: 17 3219 794 20 3745 Fragments arranged by size: 3745 3219 794 20 17 ClaI AT'CG_AT Cuts at: 1554 2187 1554 Size: 633 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2587 2622 529 Size: 35 2023 35 5702 Fragments arranged by size: 5702 2023 35 35 DraI TTT'AAA Cuts at: 5099 5099 Size: 7795 DraIII CAC_nnn'GTG Cuts at: 2049 2710 3291 3762 4098 2049 Size: 661 581 471 336 5746 Fragments arranged by size: 5746 661 581 471 336 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2978 6802 809 Size: 507 1662 3824 1802 Fragments arranged by size: 3824 1802 1662 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2697 2820 3054 Size: 54 1425 50 275 18 867 123 234 Cuts at: 3054 3135 3165 4027 4257 4582 4630 5242 6131 Size: 81 30 862 230 325 48 612 889 Cuts at: 6131 7760 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 867 862 612 325 275 234 230 123 81 54 50 48 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 5242 1537 Size: 275 3430 4090 Fragments arranged by size: 4090 3430 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3800 5725 1353 Size: 2447 1925 3423 Fragments arranged by size: 3423 2447 1925 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2984 3956 6382 6756 6902 387 Size: 1064 138 1395 972 2426 374 146 1280 Fragments arranged by size: 2426 1395 1280 1064 972 374 146 138 EcoICRI GAG'CTC Cuts at: 3577 4069 3577 Size: 492 7303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2726 2941 3622 4055 5025 5033 5858 Size: 393 1094 215 681 433 970 8 825 Cuts at: 5858 6455 7242 7510 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1094 970 825 787 681 597 433 393 268 215 8 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2741 3455 3899 4178 4469 4895 5024 2285 Size: 456 714 444 279 291 426 129 5056 Fragments arranged by size: 5056 714 456 444 426 291 279 129 EcoO109I rG'GnC_Cy Cuts at: 2734 3100 3126 3214 3953 3954 4150 4462 5029 Size: 366 26 88 739 1 196 312 567 Cuts at: 5029 2734 Size: 5500 Fragments arranged by size: 5500 739 567 366 312 196 88 26 1 EcoRI G'AATT_C Cuts at: 5104 5482 5104 Size: 378 7417 FspI TGC'GCA Cuts at: 2165 6301 2165 Size: 4136 3659 FspAI rTGC'GCAy Cuts at: 2165 2165 Size: 7795 HaeII r_GCGC'y Cuts at: 1678 2062 3716 4419 4449 6942 1678 Size: 384 1654 703 30 2493 2531 Fragments arranged by size: 2531 2493 1654 703 384 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2258 2290 2834 Size: 32 135 32 509 32 145 32 544 Cuts at: 2834 2866 2927 2959 4657 4689 5006 5028 5038 Size: 32 61 32 1698 32 317 22 10 Cuts at: 5038 5060 6436 6468 6510 6542 1373 Size: 22 1376 32 42 32 2626 Fragments arranged by size: 2626 1698 1376 544 509 317 145 135 61 42 32 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 4822 5139 5385 669 Size: 988 281 2884 317 246 3079 Fragments arranged by size: 3079 2884 988 317 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7795 HpaI GTT'AAC Cuts at: 5385 5385 Size: 7795 KpnI G_GTAC'C Cuts at: 5120 5120 Size: 7795 MfeI C'AATT_G Cuts at: 5394 5394 Size: 7795 MluI A'CGCG_T Cuts at: 7671 7671 Size: 7795 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3627 4482 4714 6909 7093 1305 Size: 2322 855 232 2195 184 2007 Fragments arranged by size: 2322 2195 2007 855 232 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2699 2822 3137 3167 4029 Size: 54 1425 343 867 123 315 30 862 Cuts at: 4029 10 Size: 3776 Fragments arranged by size: 3776 1425 867 862 343 315 123 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2304 2795 3347 3551 3734 3785 4217 Size: 648 1138 491 552 204 183 51 432 Cuts at: 4217 4328 5753 6112 6271 518 Size: 111 1425 359 159 2042 Fragments arranged by size: 2042 1425 1138 648 552 491 432 359 204 183 159 111 51 MspA1I CmG'CkG Cuts at: 1606 2055 2108 3309 3466 3642 4394 5211 5888 Size: 449 53 1201 157 176 752 817 677 Cuts at: 5888 7036 7281 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1201 1148 817 752 677 449 245 176 157 53 NaeI GCC'GGC Cuts at: 2327 2327 Size: 7795 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6304 Fragments arranged by size: 6304 939 552 NdeI CA'TA_TG Cuts at: 387 2203 387 Size: 1816 5979 NgoMIV G'CCGG_C Cuts at: 2325 2325 Size: 7795 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7795 NotI GC'GGCC_GC Cuts at: 5242 5242 Size: 7795 NspI r_CATG'y Cuts at: 1132 1229 2164 2309 2446 5153 6698 1132 Size: 97 935 145 137 2707 1545 2229 Fragments arranged by size: 2707 2229 1545 935 145 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7795 PciI A'CATG_T Cuts at: 6694 6694 Size: 7795 PflMI CCAn_nnn'nTGG Cuts at: 1108 2648 2989 3144 3940 1108 Size: 1540 341 155 796 4963 Fragments arranged by size: 4963 1540 796 341 155 PfoI T'CCnGG_A Cuts at: 2627 2742 2627 Size: 115 7680 PmeI GTTT'AAAC Cuts at: 5099 5099 Size: 7795 PmlI CAC'GTG Cuts at: 4033 4033 Size: 7795 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5864 5896 7377 7409 5864 Size: 32 1481 32 6250 Fragments arranged by size: 6250 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2734 3100 3126 3214 4150 5029 2734 Size: 366 26 88 936 879 5500 Fragments arranged by size: 5500 936 879 366 88 26 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7795 PsiI TTA'TAA Cuts at: 5365 5365 Size: 7795 PspOMI G'GGCC_C Cuts at: 2638 3953 2638 Size: 1315 6480 PstI C_TGCA'G Cuts at: 830 3269 5147 830 Size: 2439 1878 3478 Fragments arranged by size: 3478 2439 1878 PvuI CG_AT'CG Cuts at: 2001 2187 2001 Size: 186 7609 PvuII CAG'CTG Cuts at: 2108 3309 3466 3642 2108 Size: 1201 157 176 6261 Fragments arranged by size: 6261 1201 176 157 SacI G_AGCT'C Cuts at: 3579 4071 3579 Size: 492 7303 SalI G'TCGA_C Cuts at: 1936 5137 1936 Size: 3201 4594 SanDI GG'GwC_CC Cuts at: 3126 3126 Size: 7795 SapI GCTCTTCn'nnn_ Cuts at: 3800 3800 Size: 7795 SbfI CC_TGCA'GG Cuts at: 5147 5147 Size: 7795 ScaI AGT'ACT Cuts at: 6043 7755 6043 Size: 1712 6083 SexAI A'CCwGG_T Cuts at: 3758 3758 Size: 7795 SfcI C'TryA_G Cuts at: 826 1045 3108 3265 5143 6278 6959 7150 826 Size: 219 2063 157 1878 1135 681 191 1471 Fragments arranged by size: 2063 1878 1471 1135 681 219 191 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7795 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7795 SmaI CCC'GGG Cuts at: 1872 5122 1872 Size: 3250 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 3095 5899 6800 7062 820 Size: 197 926 339 813 2804 901 262 1553 Fragments arranged by size: 2804 1553 926 901 813 339 262 197 SnaBI TAC'GTA Cuts at: 493 5586 493 Size: 5093 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7795 SphI G_CATG'C Cuts at: 1132 2164 2309 2446 5153 1132 Size: 1032 145 137 2707 3774 Fragments arranged by size: 3774 2707 1032 145 137 SspI AAT'ATT Cuts at: 5 52 5719 5 Size: 47 5667 2081 Fragments arranged by size: 5667 2081 47 StuI AGG'CCT Cuts at: 1517 2562 2942 4124 4554 1517 Size: 1045 380 1182 430 4758 Fragments arranged by size: 4758 1182 1045 430 380 StyI C'CwwG_G Cuts at: 513 1065 2004 2853 3120 4458 4579 5234 513 Size: 552 939 849 267 1338 121 655 3074 Fragments arranged by size: 3074 1338 939 849 655 552 267 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 2527 2681 2773 2794 3311 3663 Size: 1255 660 287 154 92 21 517 352 Cuts at: 3663 5822 5839 5992 6177 6622 325 Size: 2159 17 153 185 445 1498 Fragments arranged by size: 2159 1498 1255 660 517 445 352 287 185 154 153 92 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4998 6041 7753 Size: 274 80 33 51 1443 3021 1043 1712 Cuts at: 7753 96 Size: 138 Fragments arranged by size: 3021 1712 1443 1043 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4332 4364 7755 7787 4332 Size: 32 3391 32 4340 Fragments arranged by size: 4340 3391 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5020 1316 Size: 3704 4091 XbaI T'CTAG_A Cuts at: 5131 5131 Size: 7795 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 2905 3064 3160 3834 4216 2707 Size: 198 159 96 674 382 6286 Fragments arranged by size: 6286 674 382 198 159 96 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7795 XmaI C'CCGG_G Cuts at: 1870 5120 1870 Size: 3250 4545 XmnI GAAnn'nnTTC Cuts at: 5924 5924 Size: 7795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5603 276 Size: 53 83 186 936 4069 2468 Fragments arranged by size: 4069 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI