Restriction map for
pFN21AB7293
(Linear) (Six-base) MAPSORT of: KIBB7293.seq Check: 1937 from: 1 to: 3090
KIBB7293.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 305 2883 3090
Size: 305 2578 207
Fragments arranged by size:
2578 305 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1617 3090
Size: 936 681 1473
Fragments arranged by size:
1473 936 681
AflIII A'CryG_T
Cuts at: 0 2342 3090
Size: 2342 748
AhdI GACnn_n'nnGTC
Cuts at: 0 3037 3090
Size: 3037 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 313 3090
Size: 313 2777
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1935 2437 3090
Size: 861 1074 502 653
Fragments arranged by size:
1074 861 653 502
ApaI G_GGCC'C
Cuts at: 0 637 1952 3090
Size: 637 1315 1138
Fragments arranged by size:
1315 1138 637
ApaLI G'TGCA_C
Cuts at: 0 1287 3090
Size: 1287 1803
AvaI C'yCGr_G
Cuts at: 0 388 2188 2735 2927 3090
Size: 388 1800 547 192 163
Fragments arranged by size:
1800 547 388 192 163
BanI G'GyrC_C
Cuts at: 0 189 367 604 750 987 1012 1240 1987
Size: 189 178 237 146 237 25 228 747
Cuts at: 1987 2419 3090
Size: 432 671
Fragments arranged by size:
747 671 432 237 237 228 189 178
146 25
BanII G_rGCy'C
Cuts at: 0 92 236 637 652 1514 1574 1840 1952
Size: 92 144 401 15 862 60 266 112
Cuts at: 1952 2066 3090
Size: 114 1024
Fragments arranged by size:
1024 862 401 266 144 114 112 92
60 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1428 1910 2270 2518 3090
Size: 1428 482 360 248 572
Fragments arranged by size:
1428 572 482 360 248
BbvCI CC'TCA_GC
Cuts at: 0 1873 2005 3090
Size: 1873 132 1085
Fragments arranged by size:
1873 1085 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 3090
Size: 1002 34 2054
Fragments arranged by size:
2054 1002 34
BclI T'GATC_A
Cuts at: 0 1367 3090
Size: 1367 1723
BglI GCCn_nnn'nGGC
Cuts at: 0 996 1313 3090
Size: 996 317 1777
Fragments arranged by size:
1777 996 317
BglII A'GATC_T
Cuts at: 0 2846 3090
Size: 2846 244
BlpI GC'TnA_GC
Cuts at: 0 76 1568 2674 2741 3090
Size: 76 1492 1106 67 349
Fragments arranged by size:
1492 1106 349 76 67
Bme1580I G_kGCm'C
Cuts at: 0 194 637 753 1291 1952 1990 2087 2983
Size: 194 443 116 538 661 38 97 896
Cuts at: 2983 3090
Size: 107
Fragments arranged by size:
896 661 538 443 194 116 107 97
38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1923 2698 3090
Size: 1923 775 392
Fragments arranged by size:
1923 775 392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 2050 3020 3028 3090
Size: 721 1329 970 8 62
Fragments arranged by size:
1329 970 721 62 8
Bpu10I CC'TnA_GC
Cuts at: 0 1873 2005 2060 3090
Size: 1873 132 55 1030
Fragments arranged by size:
1873 1030 132 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1075 3090
Size: 262 813 2015
Fragments arranged by size:
2015 813 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 712 801 888 1668 2191 3004 3090
Size: 712 89 87 780 523 813 86
Fragments arranged by size:
813 780 712 523 89 87 86
BsaAI yAC'GTr
Cuts at: 0 2028 3090
Size: 2028 1062
BsaBI GATnn'nnATC
Cuts at: 0 520 3090
Size: 520 2570
BsaHI Gr'CG_yC
Cuts at: 0 25 35 2159 3090
Size: 25 10 2124 931
Fragments arranged by size:
2124 931 25 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 829 859 922 952 3001 3031
Size: 253 30 546 30 63 30 2049 30
Cuts at: 3031 3090
Size: 59
Fragments arranged by size:
2049 546 253 63 59 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 1389 1738 1830 2485 2548 2601 2872
Size: 406 983 349 92 655 63 53 271
Cuts at: 2872 3090
Size: 218
Fragments arranged by size:
983 655 406 349 271 218 92 63
53
BseYI C'CCAG_C
Cuts at: 0 124 221 325 410 1235 1292 2385 2983
Size: 124 97 104 85 825 57 1093 598
Cuts at: 2983 3090
Size: 107
Fragments arranged by size:
1093 825 598 124 107 104 97 85
57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 250 3090
Size: 66 184 2840
Fragments arranged by size:
2840 184 66
BsiEI CG_ry'CG
Cuts at: 0 182 3090
Size: 182 2908
BsiHKAI G_wGCw'C
Cuts at: 0 206 1291 1574 1790 1943 2033 2066 2747
Size: 206 1085 283 216 153 90 33 681
Cuts at: 2747 3074 3090
Size: 327 16
Fragments arranged by size:
1085 681 327 283 216 206 153 90
33 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 2924 3090
Size: 16 2908 166
Fragments arranged by size:
2908 166 16
Bsp1286I G_dGCh'C
Cuts at: 0 92 194 206 236 637 652 753 1291
Size: 92 102 12 30 401 15 101 538
Cuts at: 1291 1514 1574 1790 1840 1943 1952 1990 2033
Size: 223 60 216 50 103 9 38 43
Cuts at: 2033 2066 2087 2747 2983 3074 3090
Size: 33 21 660 236 91 16
Fragments arranged by size:
660 538 401 236 223 216 103 102
101 92 91 60 50 43 38 33
30 21 16 15 12 9
BspHI T'CATG_A
Cuts at: 0 2318 3090
Size: 2318 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 305 476 740 1535 2173 2883 3090
Size: 272 33 171 264 795 638 710 207
Fragments arranged by size:
795 710 638 272 264 207 171 33
BsrDI GCAATG_nn'
Cuts at: 0 785 2135 3090
Size: 785 1350 955
Fragments arranged by size:
1350 955 785
BsrFI r'CCGG_y
Cuts at: 0 320 3090
Size: 320 2770
BsrGI T'GTAC_A
Cuts at: 0 2993 3090
Size: 2993 97
BssSI C'ACGA_G
Cuts at: 0 331 3090
Size: 331 2759
BstAPI GCAn_nnn'nTGC
Cuts at: 0 152 2055 3090
Size: 152 1903 1035
Fragments arranged by size:
1903 1035 152
BstEII G'GTnAC_C
Cuts at: 0 540 1084 3090
Size: 540 544 2006
Fragments arranged by size:
2006 544 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 745 825 1344 1548 1782 2211 2460
Size: 684 61 80 519 204 234 429 249
Cuts at: 2460 3090
Size: 630
Fragments arranged by size:
684 630 519 429 249 234 204 80
61
BstYI r'GATC_y
Cuts at: 0 2165 2606 2846 3090
Size: 2165 441 240 244
Fragments arranged by size:
2165 441 244 240
BtgI C'CryG_G
Cuts at: 0 683 791 1730 3090
Size: 683 108 939 1360
Fragments arranged by size:
1360 939 683 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1527 1548 1755 2058 3090
Size: 1527 21 207 303 1032
Fragments arranged by size:
1527 1032 303 207 21
BtsI GCAGTG_nn'
Cuts at: 0 70 87 3090
Size: 70 17 3003
Fragments arranged by size:
3003 70 17
ClaI AT'CG_AT
Cuts at: 0 182 3090
Size: 182 2908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 3090
Size: 582 35 2473
Fragments arranged by size:
2473 582 35
DraIII CAC_nnn'GTG
Cuts at: 0 44 705 1286 1757 2093 3090
Size: 44 661 581 471 336 997
Fragments arranged by size:
997 661 581 471 336 44
DrdI GACnn_nn'nnGTC
Cuts at: 0 973 3090
Size: 973 2117
EaeI y'GGCC_r
Cuts at: 0 692 815 1049 1130 1160 2022 2252 2577
Size: 692 123 234 81 30 862 230 325
Cuts at: 2577 2625 3090
Size: 48 465
Fragments arranged by size:
862 692 465 325 234 230 123 81
48 30
EarI CTCTTCn'nnn_
Cuts at: 0 1795 3090
Size: 1795 1295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 979 1951 3090
Size: 979 972 1139
Fragments arranged by size:
1139 979 972
EcoICRI GAG'CTC
Cuts at: 0 1572 2064 3090
Size: 1572 492 1026
Fragments arranged by size:
1572 1026 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 936 1617 2050 3020 3028 3090
Size: 721 215 681 433 970 8 62
Fragments arranged by size:
970 721 681 433 215 62 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 736 1450 1894 2173 2464 2890 3019
Size: 280 456 714 444 279 291 426 129
Cuts at: 3019 3090
Size: 71
Fragments arranged by size:
714 456 444 426 291 280 279 129
71
EcoO109I rG'GnC_Cy
Cuts at: 0 729 1095 1121 1209 1948 1949 2145 2457
Size: 729 366 26 88 739 1 196 312
Cuts at: 2457 3024 3090
Size: 567 66
Fragments arranged by size:
739 729 567 366 312 196 88 66
26 1
FspI TGC'GCA
Cuts at: 0 160 3090
Size: 160 2930
FspAI rTGC'GCAy
Cuts at: 0 160 3090
Size: 160 2930
HaeII r_GCGC'y
Cuts at: 0 57 1711 2414 2444 3090
Size: 57 1654 703 30 646
Fragments arranged by size:
1654 703 646 57 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 253 285 829 861 922 954
Size: 76 32 145 32 544 32 61 32
Cuts at: 954 2652 2684 3001 3023 3033 3055 3090
Size: 1698 32 317 22 10 22 35
Fragments arranged by size:
1698 544 317 145 76 61 35 32
32 32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 2817 3090
Size: 2817 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1622 2477 2709 3090
Size: 1622 855 232 381
Fragments arranged by size:
1622 855 381 232
MscI TGG'CCA
Cuts at: 0 694 817 1132 1162 2024 3090
Size: 694 123 315 30 862 1066
Fragments arranged by size:
1066 862 694 315 123 30
MslI CAynn'nnrTG
Cuts at: 0 299 790 1342 1546 1729 1780 2212 2323
Size: 299 491 552 204 183 51 432 111
Cuts at: 2323 3090
Size: 767
Fragments arranged by size:
767 552 491 432 299 204 183 111
51
MspA1I CmG'CkG
Cuts at: 0 50 103 1304 1461 1637 2389 3090
Size: 50 53 1201 157 176 752 701
Fragments arranged by size:
1201 752 701 176 157 53 50
NaeI GCC'GGC
Cuts at: 0 322 3090
Size: 322 2768
NdeI CA'TA_TG
Cuts at: 0 198 3090
Size: 198 2892
NgoMIV G'CCGG_C
Cuts at: 0 320 3090
Size: 320 2770
NspI r_CATG'y
Cuts at: 0 159 304 441 3090
Size: 159 145 137 2649
Fragments arranged by size:
2649 159 145 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 643 984 1139 1935 3090
Size: 643 341 155 796 1155
Fragments arranged by size:
1155 796 643 341 155
PfoI T'CCnGG_A
Cuts at: 0 622 737 3090
Size: 622 115 2353
Fragments arranged by size:
2353 622 115
PmlI CAC'GTG
Cuts at: 0 2028 3090
Size: 2028 1062
PpuMI rG'GwC_Cy
Cuts at: 0 729 1095 1121 1209 2145 3024 3090
Size: 729 366 26 88 936 879 66
Fragments arranged by size:
936 879 729 366 88 66 26
PspOMI G'GGCC_C
Cuts at: 0 633 1948 3090
Size: 633 1315 1142
Fragments arranged by size:
1315 1142 633
PstI C_TGCA'G
Cuts at: 0 1264 3090
Size: 1264 1826
PvuI CG_AT'CG
Cuts at: 0 182 3090
Size: 182 2908
PvuII CAG'CTG
Cuts at: 0 103 1304 1461 1637 3090
Size: 103 1201 157 176 1453
Fragments arranged by size:
1453 1201 176 157 103
SacI G_AGCT'C
Cuts at: 0 1574 2066 3090
Size: 1574 492 1024
Fragments arranged by size:
1574 1024 492
SanDI GG'GwC_CC
Cuts at: 0 1121 3090
Size: 1121 1969
SapI GCTCTTCn'nnn_
Cuts at: 0 1795 3090
Size: 1795 1295
SexAI A'CCwGG_T
Cuts at: 0 1753 3090
Size: 1753 1337
SfcI C'TryA_G
Cuts at: 0 1103 1260 3090
Size: 1103 157 1830
Fragments arranged by size:
1830 1103 157
SmlI C'TyrA_G
Cuts at: 0 277 1090 3090
Size: 277 813 2000
Fragments arranged by size:
2000 813 277
SphI G_CATG'C
Cuts at: 0 159 304 441 3090
Size: 159 145 137 2649
Fragments arranged by size:
2649 159 145 137
StuI AGG'CCT
Cuts at: 0 557 937 2119 2549 3090
Size: 557 380 1182 430 541
Fragments arranged by size:
1182 557 541 430 380
StyI C'CwwG_G
Cuts at: 0 848 1115 2453 2574 3090
Size: 848 267 1338 121 516
Fragments arranged by size:
1338 848 516 267 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 522 676 768 789 1306 1658 3090
Size: 235 287 154 92 21 517 352 1432
Fragments arranged by size:
1432 517 352 287 235 154 92 21
TatI w'GTAC_w
Cuts at: 0 2993 3090
Size: 2993 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2327 2359 3090
Size: 2327 32 731
Fragments arranged by size:
2327 731 32
Tth111I GACn'n_nGTC
Cuts at: 0 3015 3090
Size: 3015 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 900 1059 1155 1829 2211 3090
Size: 702 198 159 96 674 382 879
Fragments arranged by size:
879 702 674 382 198 159 96
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuI PvuII
SacI SanDI SapI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BmgBI BmtI BplI BsaWI BsiWI
BsmI BspEI BsrBI BssHII BstBI BstZ17I Bsu36I DraI
EagI EcoRI EcoRV FalI FseI HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI RsrII SacII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI