(Circular) (Six-base) MAPSORT of: pFN21AB7269.seq Check: 9495 from: 1 to: 5998 pFN21AB7269 5998 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 2340 4125 4498 1563 Size: 777 1785 373 3063 Fragments arranged by size: 3063 1785 777 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2313 2396 3093 4061 5445 5713 1632 Size: 442 239 83 697 968 1384 268 1917 Fragments arranged by size: 1917 1384 968 697 442 268 239 83 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2967 2999 2967 Size: 32 5966 AlwNI CAG_nnn'CTG Cuts at: 3741 5313 3741 Size: 1572 4426 ApaI G_GGCC'C Cuts at: 2198 2598 2198 Size: 400 5598 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 2829 3169 3307 3533 3685 5794 1493 Size: 1336 340 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1336 340 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3323 1870 Size: 73 1380 4545 Fragments arranged by size: 4545 1380 73 BamHI G'GATC_C Cuts at: 2661 3095 3328 3695 2661 Size: 434 233 367 4964 Fragments arranged by size: 4964 434 367 233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 4774 618 Size: 325 217 627 1532 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2198 2598 3317 1391 Size: 807 400 719 4072 Fragments arranged by size: 4072 807 719 400 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3223 928 Size: 971 1324 3703 Fragments arranged by size: 3703 1324 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4609 136 Size: 107 122 71 631 605 2937 1525 Fragments arranged by size: 2937 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2198 2598 4059 5215 1790 Size: 408 400 1461 1156 2573 Fragments arranged by size: 2573 1461 1156 408 400 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2511 2847 2931 Size: 416 53 83 399 24 1094 336 84 Cuts at: 2931 3049 3260 3729 4687 442 Size: 118 211 469 958 1753 Fragments arranged by size: 1753 1094 958 469 416 399 336 211 118 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2780 2348 Size: 432 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2103 2192 2691 3051 3074 4658 1239 Size: 864 89 499 360 23 1584 2579 Fragments arranged by size: 2579 1584 864 499 360 89 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5773 Fragments arranged by size: 5773 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2109 3052 3283 1186 Size: 923 943 231 3901 Fragments arranged by size: 3901 943 923 231 BseYI C'CCAG_C Cuts at: 5201 5697 5201 Size: 496 5502 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3062 3153 4059 4144 5215 3062 Size: 91 906 85 1071 3845 Fragments arranged by size: 3845 1071 906 91 85 BsmI GAATG_Cn' Cuts at: 2319 3509 3602 2319 Size: 1190 93 4715 Fragments arranged by size: 4715 1190 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2598 3062 3153 3317 4059 4144 Size: 399 408 400 464 91 164 742 85 Cuts at: 4144 5215 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 464 408 400 399 164 91 85 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2739 3353 844 Size: 1317 578 614 3489 Fragments arranged by size: 3489 1317 614 578 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 2566 96 Size: 2470 3528 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 794 Size: 1520 4478 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2205 2205 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2200 2661 3095 3328 3695 4080 4097 5549 Size: 228 461 434 233 367 385 17 1452 Cuts at: 5549 5635 5647 5993 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 461 434 385 367 346 233 228 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2189 513 Size: 552 1124 4322 Fragments arranged by size: 4322 1124 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 3514 4308 4328 3514 Size: 794 20 5184 Fragments arranged by size: 5184 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 2362 3302 2362 Size: 940 5058 DraIII CAC_nnn'GTG Cuts at: 3007 3007 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3445 4334 5963 Size: 54 1425 50 275 18 1615 889 1629 Cuts at: 5963 8 Size: 43 Fragments arranged by size: 1629 1615 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3928 1353 Size: 2575 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2012 4585 4959 5105 387 Size: 1064 138 423 2573 374 146 1280 Fragments arranged by size: 2573 1280 1064 423 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2103 2192 2313 2396 2691 3051 Size: 393 442 29 89 121 83 295 360 Cuts at: 3051 3074 3093 4061 4658 5445 5713 1239 Size: 23 19 968 597 787 268 1524 Fragments arranged by size: 1524 968 787 597 442 393 360 295 268 121 89 83 29 23 19 EcoO109I rG'GnC_Cy Cuts at: 2194 2594 2595 3028 2194 Size: 400 1 433 5164 Fragments arranged by size: 5164 433 400 1 EcoRI G'AATT_C Cuts at: 2829 3307 3685 2829 Size: 478 378 5142 Fragments arranged by size: 5142 478 378 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2374 2406 2638 2670 4639 Size: 32 135 32 802 32 232 32 1969 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1969 802 232 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 2474 2538 2790 748 Size: 1726 64 252 3956 Fragments arranged by size: 3956 1726 252 64 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2765 5112 5296 1305 Size: 1460 2347 184 2007 Fragments arranged by size: 2347 2007 1460 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4176 Fragments arranged by size: 4176 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3956 4315 4474 518 Size: 648 2790 359 159 2042 Fragments arranged by size: 2790 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3414 4091 5239 5484 1606 Size: 1808 677 1148 245 2120 Fragments arranged by size: 2120 1808 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2189 513 Size: 552 1124 4322 Fragments arranged by size: 4322 1124 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3146 3356 4901 1132 Size: 97 1917 210 1545 2229 Fragments arranged by size: 2229 1917 1545 210 97 PasI CC'CwG_GG Cuts at: 1348 2205 1348 Size: 857 5141 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PfoI T'CCnGG_A Cuts at: 2249 2249 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3028 3028 Size: 5998 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 2276 3568 2276 Size: 1292 4706 PspOMI G'GGCC_C Cuts at: 2194 2594 2194 Size: 400 5598 PstI C_TGCA'G Cuts at: 830 2736 3350 830 Size: 1906 614 3478 Fragments arranged by size: 3478 1906 614 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2732 3346 4481 5162 5353 826 Size: 219 1687 614 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 681 614 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4102 5003 5265 820 Size: 197 926 2159 901 262 1553 Fragments arranged by size: 2159 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2074 2189 3031 3437 513 Size: 552 1009 115 842 406 3074 Fragments arranged by size: 3074 1009 842 552 406 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2897 4025 4042 4195 4380 4825 325 Size: 1255 1317 1128 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 1128 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2566 4244 5956 Size: 274 80 33 51 1443 589 1678 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1712 1678 1443 589 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5958 5990 5958 Size: 32 5966 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 2456 2792 2960 3173 3202 4127 2456 Size: 336 168 213 29 925 4327 Fragments arranged by size: 4327 925 336 213 168 29 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI