Restriction map for
pFN21AB7267
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AspI BamHI BbrPI
BciVI BclI BglII BlpI BpmI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
HphI Ksp632I KspI MamI MfeI
MflI MluI MroI MslI MspA1I
MunI NcoI NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 213 GGTACC G^GTACC
AfaI 214 GTAC GT^AC
AflII 67 CTTAAG C^TTAAG
AflIII 588 ACACGT A^C[GA][CT]GT
AgeI 216 ACCGGT A^CCGGT
Alw44I 727 GTGCAC G^TGCAC
AlwI 818 GGATC GGATC(4/5)
AlwNI 790 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 813 CCTGAGG CC^T[ACGT]AGG
Aor51HI 911 AGCGCT AGC^GCT
ApaI 414 GGGCCC GGGCC^C
ApaLI 727 GTGCAC G^TGCAC
Asp718I 213 GGTACC G^GTACC
AspHI 727 GTGCAC G[AT]GC[AT]^C
AviII 469 TGCGCA TGC^GCA
BalI 806 TGGCCA TGG^CCA
BbsI 36 GAAGAC GAAGAC(2/6)
BbvCI 888 CCTCAGC CCTCAGC(-5/-2)
BfrI 67 CTTAAG C^TTAAG
BpuAI 36 GAAGAC GAAGAC(2/6)
BsaAI 589 CACGTA [CT]AC^GT[GA]
BsaI 309 GGTCTC GGTCTC(1/5)
BsaWI 216 ACCGGT [AT]^CCGG[AT]
BseRI 660 CTCCTC GAGGAG(10/8)
BsiHKAI 727 GTGCAC G[AT]GC[AT]^C
BsmAI 310 GTCTC GTCTC(1/5)
BspMI 765 GCAGGT ACCTGC(4/8)
BsrBI 715 GAGCGG CCGCTC(-3/-3)
BstXI 402 CCAGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 813 CCTGAGG CC^T[ACGT]AGG
DsaI 31 CCGTGG C^C[GA][CT]GG
EagI 245 CGGCCG C^GGCCG
EclXI 245 CGGCCG C^GGCCG
Eco47III 911 AGCGCT AGC^GCT
Eco52I 245 CGGCCG C^GGCCG
Eco81I 813 CCTGAGG CC^T[ACGT]AGG
FspI 469 TGCGCA TGC^GCA
KpnI 213 GGTACC GGTAC^C
MaeIII 827 GTAAC ^GT[ACGT]AC
MboII 36 GAAGA GAAGA(8/7)
MluNI 806 TGGCCA TGG^CCA
MscI 806 TGGCCA TGG^CCA
MseI 68 TTAA T^TAA
NdeI 782 CATATG CA^TATG
PaeR7I 19 CTCGAG C^TCGAG
PinAI 216 ACCGGT A^CCGGT
PpuMI 187 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 214 GTAC GT^AC
SbfI 927 CCTGCAGG CCTGCA^GG
SfaNI 458 GCATC GCATC(5/9)
SmaI 559 CCCGGG CCC^GGG
Sse8387I 927 CCTGCAGG CCTGCA^GG
TfiI 613 GATTC G^A[AT]TC
Tru9I 68 TTAA T^TAA
XhoI 19 CTCGAG C^TCGAG
XmaCI 559 CCCGGG C^CCGGG
XmaI 559 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 240 GGCGCC G[GA]^CG[CT]C
300 GGCGCC
AvrII 339 CCTAGG C^CTAGG
628 CCTAGG
BbeI 240 GGCGCC GGCGC^C
300 GGCGCC
BcgI 645 CGAAGCGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
936 CGAGGGACGTGC
BfaI 340 CTAG C^TAG
629 CTAG
BglI 96 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
745 GCCTTCGAGGC
BlnI 339 CCTAGG C^CTAGG
628 CCTAGG
BsaHI 240 GGCGCC G[GA]^CG[CT]C
300 GGCGCC
BsgI 915 CTGCAC GTGCAG(16/14)
907 GTGCAG
BsiEI 245 CGGCCG CG[GA][CT]^CG
637 CGACCG
BssHII 105 GCGCGC G^CGCGC
481 GCGCGC
DpnII 669 GATC ^GATC
819 GATC
DraII 187 AGGACCC [GA]G^G[ACGT]CC[CT]
413 GGGGCCC
EaeI 245 CGGCCG [CT]^GGCC[GA]
806 TGGCCA
EcoO109I 187 AGGACCC [GA]G^G[ACGT]CC[CT]
413 GGGGCCC
EcoT14I 339 CCTAGG C^C[AT][AT]GG
628 CCTAGG
Hin1I 240 GGCGCC G[GA]^CG[CT]C
300 GGCGCC
KasI 240 GGCGCC G^GCGCC
300 GGCGCC
MaeI 340 CTAG C^TAG
629 CTAG
MaeII 590 ACGT A^CGT
942 ACGT
MboI 669 GATC ^GATC
819 GATC
NaeI 96 GCCGGC GCC^GGC
243 GCCGGC
NarI 240 GGCGCC GG^CGCC
300 GGCGCC
NdeII 669 GATC ^GATC
819 GATC
NgoMIV 96 GCCGGC G^CCGGC
243 GCCGGC
PleI 359 GACTC GAGTC(4/5)
526 GAGTC
Sau3AI 669 GATC ^GATC
819 GATC
SfoI 240 GGCGCC GGC^GCC
300 GGCGCC
StyI 339 CCTAGG C^C[AT][AT]GG
628 CCTAGG
TaqI 20 TCGA T^CGA
749 TCGA
Tsp509I 582 AATT ^AATT
594 AATT
TthHB8I 20 TCGA T^CGA
749 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MnlI MspI MvaI MvnI MwoI
NciI NlaIV PstI Sau96I ScrFI
SfcI SmlI TseI TspRI