(Circular) (Six-base) MAPSORT of: pFN21AB7267.seq Check: 1429 from: 1 to: 5668 pFN21AB7267 5668 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2761 2761 Size: 5668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2218 2989 2218 Size: 771 4897 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 5115 5383 1632 Size: 2099 1384 268 1917 Fragments arranged by size: 2099 1917 1384 268 AfeI AGC'GCT Cuts at: 2918 2918 Size: 5668 AflII C'TTAA_G Cuts at: 820 1017 2072 820 Size: 197 1055 4416 Fragments arranged by size: 4416 1055 197 AflIII A'CryG_T Cuts at: 1203 2593 4567 5544 1203 Size: 1390 1974 977 1327 Fragments arranged by size: 1974 1390 1327 977 AgeI A'CCGG_T Cuts at: 2221 4499 2221 Size: 2278 3390 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2800 3411 4983 2800 Size: 611 1572 3485 Fragments arranged by size: 3485 1572 611 ApaI G_GGCC'C Cuts at: 2423 2423 Size: 5668 ApaLI G'TGCA_C Cuts at: 2732 3725 4881 2732 Size: 993 1156 3519 Fragments arranged by size: 3519 1156 993 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2153 2301 2309 2564 2993 1870 Size: 73 81 129 148 8 255 429 4545 Fragments arranged by size: 4545 429 255 148 129 81 73 8 AvrII C'CTAG_G Cuts at: 2344 2633 2344 Size: 289 5379 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 2224 2230 2245 2305 Size: 325 217 627 431 6 6 15 60 Cuts at: 2305 2410 2773 2989 4444 618 Size: 105 363 216 1455 1842 Fragments arranged by size: 1842 1455 627 431 363 325 217 216 105 60 15 6 6 BanII G_rGCy'C Cuts at: 1391 2160 2300 2423 2987 1391 Size: 769 140 123 564 4072 Fragments arranged by size: 4072 769 564 140 123 BbeI G_GCGC'C Cuts at: 2249 2309 2249 Size: 60 5608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 928 Size: 971 149 4548 Fragments arranged by size: 4548 971 149 BbvCI CC'TCA_GC Cuts at: 2894 2894 Size: 5668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2639 2673 2930 2964 3859 3893 1903 Size: 34 702 34 257 34 895 34 3678 Fragments arranged by size: 3678 895 702 257 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2107 2756 4279 Size: 107 122 71 631 605 435 649 1523 Cuts at: 4279 136 Size: 1525 Fragments arranged by size: 1525 1523 649 631 605 435 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 2273 2423 2736 3729 4885 1790 Size: 483 150 313 993 1156 2573 Fragments arranged by size: 2573 1156 993 483 313 150 BmgBI CAC'GTC Cuts at: 1318 2948 1318 Size: 1630 4038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2234 3399 4357 Size: 416 53 83 399 24 817 1165 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1165 958 817 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4328 1239 Size: 3089 2579 Bpu10I CC'TnA_GC Cuts at: 2762 2894 2762 Size: 132 5536 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 3793 4658 4956 2066 Size: 1727 865 298 2778 Fragments arranged by size: 2778 1727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2320 4331 882 Size: 617 821 2011 2219 Fragments arranged by size: 2219 2011 821 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 3459 493 Size: 711 1392 863 2702 Fragments arranged by size: 2702 1392 863 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2246 2306 3475 3857 Size: 53 83 186 936 713 60 1169 382 Cuts at: 3857 4492 275 Size: 635 1451 Fragments arranged by size: 1451 1169 936 713 635 382 186 83 60 53 BsaWI w'CCGG_w Cuts at: 2221 4101 4499 4773 4920 5331 2221 Size: 1880 398 274 147 411 2558 Fragments arranged by size: 2558 1880 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2656 1186 Size: 1470 4198 BseYI C'CCAG_C Cuts at: 2509 4871 5367 2509 Size: 2362 496 2810 Fragments arranged by size: 2810 2362 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2905 2933 1163 Size: 573 1169 28 3898 Fragments arranged by size: 3898 1169 573 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 2645 3118 3879 4907 1540 Size: 275 186 252 392 473 761 1028 2301 Fragments arranged by size: 2301 1028 761 473 392 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2736 3729 3814 4885 2736 Size: 993 85 1071 3519 Fragments arranged by size: 3519 1071 993 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2273 2300 2423 2736 2987 3729 Size: 399 370 113 27 123 313 251 742 Cuts at: 3729 3814 4885 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 313 251 123 113 85 27 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2761 3023 844 Size: 1917 262 3489 Fragments arranged by size: 3489 1917 262 BsrBI CCG'CTC Cuts at: 1394 1823 2722 3555 1394 Size: 429 899 833 3507 Fragments arranged by size: 3507 899 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2209 2221 2227 2248 4312 4499 Size: 110 291 108 12 6 21 2064 187 Cuts at: 4499 1700 Size: 2869 Fragments arranged by size: 2869 2064 291 187 110 108 21 12 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 2110 2486 1925 Size: 185 376 5107 Fragments arranged by size: 5107 376 185 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstXI CCAn_nnnn'nTGG Cuts at: 2414 2414 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2819 2819 Size: 5668 BtgI C'CryG_G Cuts at: 513 1065 2036 513 Size: 552 971 4145 Fragments arranged by size: 4145 971 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2358 3184 3978 3998 2358 Size: 826 794 20 4028 Fragments arranged by size: 4028 826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2811 3115 Size: 54 1425 50 275 18 420 561 304 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 561 420 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 3115 1537 Size: 275 438 865 4090 Fragments arranged by size: 4090 865 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3731 4328 5115 5383 1239 Size: 393 2099 597 787 268 1524 Fragments arranged by size: 2099 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2193 2419 2193 Size: 226 5442 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 2476 4174 2476 Size: 1698 3970 FspAI rTGC'GCAy Cuts at: 2476 2476 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2153 2249 2309 2525 2762 2920 4815 1678 Size: 475 96 60 216 237 158 1895 2531 Fragments arranged by size: 2531 1895 475 237 216 158 96 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KasI G'GCGC_C Cuts at: 2245 2305 2245 Size: 60 5608 KpnI G_GTAC'C Cuts at: 2222 2993 2222 Size: 771 4897 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2158 2343 4782 4966 1305 Size: 853 185 2439 184 2007 Fragments arranged by size: 2439 2007 853 185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 10 Size: 54 1425 343 981 2865 Fragments arranged by size: 2865 1425 981 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3626 3985 4144 518 Size: 648 2460 359 159 2042 Fragments arranged by size: 2460 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3084 3761 4909 5154 1606 Size: 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 245 NaeI GCC'GGC Cuts at: 2103 2250 2103 Size: 147 5521 NarI GG'CG_CC Cuts at: 2246 2306 2246 Size: 60 5608 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5116 NdeI CA'TA_TG Cuts at: 387 2788 387 Size: 2401 3267 NgoMIV G'CCGG_C Cuts at: 2101 2248 2101 Size: 147 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PfoI T'CCnGG_A Cuts at: 2439 2439 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2193 2193 Size: 5668 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspOMI G'GGCC_C Cuts at: 2419 2419 Size: 5668 PspXI vC'TCGA_Gb Cuts at: 2024 2024 Size: 5668 PstI C_TGCA'G Cuts at: 830 2208 2688 2937 3020 830 Size: 1378 480 249 83 3478 Fragments arranged by size: 3478 1378 480 249 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 2937 3020 2937 Size: 83 5585 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2204 2684 2872 2933 3016 4151 4832 Size: 219 1159 480 188 61 83 1135 681 Cuts at: 4832 5023 826 Size: 191 1471 Fragments arranged by size: 1471 1159 1135 681 480 219 191 188 83 61 SfoI GGC'GCC Cuts at: 2247 2307 2247 Size: 60 5608 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2566 2995 1872 Size: 694 429 4545 Fragments arranged by size: 4545 694 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 2072 2081 3772 4673 4935 Size: 197 926 81 48 9 1691 901 262 Cuts at: 4935 820 Size: 1553 Fragments arranged by size: 1691 1553 926 901 262 197 81 48 9 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2344 2633 3107 513 Size: 552 1279 289 474 3074 Fragments arranged by size: 3074 1279 552 474 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 2421 2453 2603 2635 5628 5660 2021 Size: 32 368 32 150 32 2993 32 2029 Fragments arranged by size: 2993 2029 368 150 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 2024 1943 Size: 81 5587 XmaI C'CCGG_G Cuts at: 1870 2564 2993 1870 Size: 694 429 4545 Fragments arranged by size: 4545 694 429 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI