Restriction map for
pFN21AB7267
(Linear) (Six-base) MAPSORT of: KIBB7267.seq Check: 7689 from: 1 to: 963
KIBB7267.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 756 963
Size: 756 207
Acc65I G'GTAC_C
Cuts at: 0 213 963
Size: 213 750
AfeI AGC'GCT
Cuts at: 0 913 963
Size: 913 50
AflII C'TTAA_G
Cuts at: 0 67 963
Size: 67 896
AflIII A'CryG_T
Cuts at: 0 588 963
Size: 588 375
AgeI A'CCGG_T
Cuts at: 0 216 963
Size: 216 747
AlwNI CAG_nnn'CTG
Cuts at: 0 795 963
Size: 795 168
ApaI G_GGCC'C
Cuts at: 0 418 963
Size: 418 545
ApaLI G'TGCA_C
Cuts at: 0 727 963
Size: 727 236
AvaI C'yCGr_G
Cuts at: 0 19 148 296 304 559 963
Size: 19 129 148 8 255 404
Fragments arranged by size:
404 255 148 129 19 8
AvrII C'CTAG_G
Cuts at: 0 339 628 963
Size: 339 289 335
Fragments arranged by size:
339 335 289
BanI G'GyrC_C
Cuts at: 0 213 219 225 240 300 405 768 963
Size: 213 6 6 15 60 105 363 195
Fragments arranged by size:
363 213 195 105 60 15 6 6
BanII G_rGCy'C
Cuts at: 0 155 295 418 963
Size: 155 140 123 545
Fragments arranged by size:
545 155 140 123
BbeI G_GCGC'C
Cuts at: 0 244 304 963
Size: 244 60 659
Fragments arranged by size:
659 244 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 963
Size: 43 920
BbvCI CC'TCA_GC
Cuts at: 0 889 963
Size: 889 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 634 668 925 959 963
Size: 634 34 257 34 4
Fragments arranged by size:
634 257 34 34 4
BglI GCCn_nnn'nGGC
Cuts at: 0 102 751 963
Size: 102 649 212
Fragments arranged by size:
649 212 102
Bme1580I G_kGCm'C
Cuts at: 0 268 418 731 963
Size: 268 150 313 232
Fragments arranged by size:
313 268 232 150
BmgBI CAC'GTC
Cuts at: 0 943 963
Size: 943 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 229 963
Size: 229 734
Bpu10I CC'TnA_GC
Cuts at: 0 757 889 963
Size: 757 132 74
Fragments arranged by size:
757 132 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 963
Size: 61 902
BsaI GGTCTCn'nnnn_
Cuts at: 0 315 963
Size: 315 648
BsaAI yAC'GTr
Cuts at: 0 591 963
Size: 591 372
BsaHI Gr'CG_yC
Cuts at: 0 241 301 963
Size: 241 60 662
Fragments arranged by size:
662 241 60
BsaWI w'CCGG_w
Cuts at: 0 216 963
Size: 216 747
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 651 963
Size: 651 312
BseYI C'CCAG_C
Cuts at: 0 504 963
Size: 504 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 928 963
Size: 900 28 35
Fragments arranged by size:
900 35 28
BsiEI CG_ry'CG
Cuts at: 0 248 640 963
Size: 248 392 323
Fragments arranged by size:
392 323 248
BsiHKAI G_wGCw'C
Cuts at: 0 731 963
Size: 731 232
Bsp1286I G_dGCh'C
Cuts at: 0 155 268 295 418 731 963
Size: 155 113 27 123 313 232
Fragments arranged by size:
313 232 155 123 113 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 756 963
Size: 756 207
BsrBI CCG'CTC
Cuts at: 0 717 963
Size: 717 246
BsrFI r'CCGG_y
Cuts at: 0 96 204 216 222 243 963
Size: 96 108 12 6 21 720
Fragments arranged by size:
720 108 96 21 12 6
BssHII G'CGCG_C
Cuts at: 0 105 481 963
Size: 105 376 482
Fragments arranged by size:
482 376 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 409 963
Size: 409 554
Bsu36I CC'TnA_GG
Cuts at: 0 814 963
Size: 814 149
BtgI C'CryG_G
Cuts at: 0 31 963
Size: 31 932
BtsI GCAGTG_nn'
Cuts at: 0 353 963
Size: 353 610
EaeI y'GGCC_r
Cuts at: 0 245 806 963
Size: 245 561 157
Fragments arranged by size:
561 245 157
EagI C'GGCC_G
Cuts at: 0 245 963
Size: 245 718
EcoO109I rG'GnC_Cy
Cuts at: 0 188 414 963
Size: 188 226 549
Fragments arranged by size:
549 226 188
FspI TGC'GCA
Cuts at: 0 471 963
Size: 471 492
FspAI rTGC'GCAy
Cuts at: 0 471 963
Size: 471 492
HaeII r_GCGC'y
Cuts at: 0 148 244 304 520 757 915 963
Size: 148 96 60 216 237 158 48
Fragments arranged by size:
237 216 158 148 96 60 48
KasI G'GCGC_C
Cuts at: 0 240 300 963
Size: 240 60 663
Fragments arranged by size:
663 240 60
KpnI G_GTAC'C
Cuts at: 0 217 963
Size: 217 746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 338 963
Size: 153 185 625
Fragments arranged by size:
625 185 153
MscI TGG'CCA
Cuts at: 0 808 963
Size: 808 155
NaeI GCC'GGC
Cuts at: 0 98 245 963
Size: 98 147 718
Fragments arranged by size:
718 147 98
NarI GG'CG_CC
Cuts at: 0 241 301 963
Size: 241 60 662
Fragments arranged by size:
662 241 60
NdeI CA'TA_TG
Cuts at: 0 783 963
Size: 783 180
NgoMIV G'CCGG_C
Cuts at: 0 96 243 963
Size: 96 147 720
Fragments arranged by size:
720 147 96
PfoI T'CCnGG_A
Cuts at: 0 434 963
Size: 434 529
PpuMI rG'GwC_Cy
Cuts at: 0 188 963
Size: 188 775
PspOMI G'GGCC_C
Cuts at: 0 414 963
Size: 414 549
PspXI vC'TCGA_Gb
Cuts at: 0 19 963
Size: 19 944
PstI C_TGCA'G
Cuts at: 0 203 683 932 963
Size: 203 480 249 31
Fragments arranged by size:
480 249 203 31
SbfI CC_TGCA'GG
Cuts at: 0 932 963
Size: 932 31
SfcI C'TryA_G
Cuts at: 0 199 679 867 928 963
Size: 199 480 188 61 35
Fragments arranged by size:
480 199 188 61 35
SfoI GGC'GCC
Cuts at: 0 242 302 963
Size: 242 60 661
Fragments arranged by size:
661 242 60
SmaI CCC'GGG
Cuts at: 0 561 963
Size: 561 402
SmlI C'TyrA_G
Cuts at: 0 19 67 76 963
Size: 19 48 9 887
Fragments arranged by size:
887 48 19 9
StyI C'CwwG_G
Cuts at: 0 339 628 963
Size: 339 289 335
Fragments arranged by size:
339 335 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 16 48 416 448 598 630 963
Size: 16 32 368 32 150 32 333
Fragments arranged by size:
368 333 150 32 32 32 16
XhoI C'TCGA_G
Cuts at: 0 19 963
Size: 19 944
XmaI C'CCGG_G
Cuts at: 0 559 963
Size: 559 404
Enzymes that do cut:
AarI Acc65I AfeI AflII AflIII AgeI AlwNI ApaI
ApaLI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BcgI BglI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstXI Bsu36I BtgI
BtsI EaeI EagI EcoO109I FspI FspAI HaeII KasI
KpnI MmeI MscI NaeI NarI NdeI NgoMIV PfoI
PpuMI PspOMI PspXI PstI SbfI SfcI SfoI SmaI
SmlI StyI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AhdI AleI AlfI AloI
ApoI AscI AseI AsiSI BaeI BamHI BciVI BclI
BfrBI BglII BlpI BmtI BplI BpmI BsaBI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI
MfeI MluI MslI MspA1I NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XmnI ZraI