Restriction map for
pFN21AB7263
(Linear) (Six-base) MAPSORT of: KIBB7263.seq Check: 8361 from: 1 to: 945
KIBB7263.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 232 945
Size: 232 713
AflIII A'CryG_T
Cuts at: 0 724 945
Size: 724 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 309 945
Size: 309 636
ApoI r'AATT_y
Cuts at: 0 911 945
Size: 911 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 713 746 945
Size: 713 33 199
Fragments arranged by size:
713 199 33
BanII G_rGCy'C
Cuts at: 0 35 945
Size: 35 910
BciVI GTATCCnnnnn_n'
Cuts at: 0 741 945
Size: 741 204
BlpI GC'TnA_GC
Cuts at: 0 302 945
Size: 302 643
BmrI ACTGGGnnnn_n'
Cuts at: 0 853 945
Size: 853 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 257 355 945
Size: 85 172 98 590
Fragments arranged by size:
590 172 98 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 945
Size: 379 566
Bsp1286I G_dGCh'C
Cuts at: 0 35 945
Size: 35 910
BsrDI GCAATG_nn'
Cuts at: 0 525 614 945
Size: 525 89 331
Fragments arranged by size:
525 331 89
BsrGI T'GTAC_A
Cuts at: 0 571 945
Size: 571 374
BssSI C'ACGA_G
Cuts at: 0 695 945
Size: 695 250
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 945
Size: 796 149
BstYI r'GATC_y
Cuts at: 0 278 945
Size: 278 667
Bsu36I CC'TnA_GG
Cuts at: 0 46 931 945
Size: 46 885 14
Fragments arranged by size:
885 46 14
BtgI C'CryG_G
Cuts at: 0 356 513 945
Size: 356 157 432
Fragments arranged by size:
432 356 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 775 945
Size: 775 170
BtsI GCAGTG_nn'
Cuts at: 0 212 391 945
Size: 212 179 554
Fragments arranged by size:
554 212 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 231 266 945
Size: 231 35 679
Fragments arranged by size:
679 231 35
DraIII CAC_nnn'GTG
Cuts at: 0 628 945
Size: 628 317
EaeI y'GGCC_r
Cuts at: 0 353 945
Size: 353 592
EarI CTCTTCn'nnn_
Cuts at: 0 100 695 945
Size: 100 595 250
Fragments arranged by size:
595 250 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 257 355 945
Size: 85 172 98 590
Fragments arranged by size:
590 172 98 85
EcoRI G'AATT_C
Cuts at: 0 911 945
Size: 911 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 486 518 676 708 945
Size: 486 32 158 32 237
Fragments arranged by size:
486 237 158 32 32
HindIII A'AGCT_T
Cuts at: 0 459 945
Size: 459 486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 945
Size: 245 700
MscI TGG'CCA
Cuts at: 0 355 945
Size: 355 590
MslI CAynn'nnrTG
Cuts at: 0 518 551 740 829 945
Size: 518 33 189 89 116
Fragments arranged by size:
518 189 116 89 33
MspA1I CmG'CkG
Cuts at: 0 306 945
Size: 306 639
NcoI C'CATG_G
Cuts at: 0 356 513 945
Size: 356 157 432
Fragments arranged by size:
432 356 157
NspI r_CATG'y
Cuts at: 0 728 945
Size: 728 217
PciI A'CATG_T
Cuts at: 0 724 945
Size: 724 221
PflMI CCAn_nnn'nTGG
Cuts at: 0 772 945
Size: 772 173
PfoI T'CCnGG_A
Cuts at: 0 57 332 945
Size: 57 275 613
Fragments arranged by size:
613 275 57
PvuII CAG'CTG
Cuts at: 0 306 945
Size: 306 639
SapI GCTCTTCn'nnn_
Cuts at: 0 695 945
Size: 695 250
SfcI C'TryA_G
Cuts at: 0 879 945
Size: 879 66
StyI C'CwwG_G
Cuts at: 0 356 513 945
Size: 356 157 432
Fragments arranged by size:
432 356 157
TatI w'GTAC_w
Cuts at: 0 471 571 945
Size: 471 100 374
Fragments arranged by size:
471 374 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3 35 945
Size: 3 32 910
Fragments arranged by size:
910 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 945
Size: 627 318
Enzymes that do cut:
AccI AflIII AlfI ApoI BaeI BanII BciVI BlpI
BmrI BpmI BsgI Bsp1286I BsrDI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI Eco57MI EcoRI Hin4I HindIII MmeI MscI MslI
MspA1I NcoI NspI PciI PflMI PfoI PvuII SapI
SfcI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BstZ17I ClaI DraI DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI