(Circular) (Six-base) MAPSORT of: pFN21AB7252.seq Check: 1158 from: 1 to: 6148 pFN21AB7252 6148 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3228 3319 3228 Size: 91 6057 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4211 5595 5863 1632 Size: 2579 1384 268 1917 Fragments arranged by size: 2579 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 2634 1166 Size: 1468 4680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2475 2507 2778 2810 2475 Size: 32 271 32 5813 Fragments arranged by size: 5813 271 32 32 AlwNI CAG_nnn'CTG Cuts at: 2114 2542 2725 3891 5463 2114 Size: 428 183 1166 1572 2799 Fragments arranged by size: 2799 1572 1166 428 183 ApaI G_GGCC'C Cuts at: 2054 2079 2558 2702 2858 3002 2054 Size: 25 479 144 156 144 5200 Fragments arranged by size: 5200 479 156 144 144 25 ApaLI G'TGCA_C Cuts at: 2898 4205 5361 2898 Size: 1307 1156 3685 Fragments arranged by size: 3685 1307 1156 ApoI r'AATT_y Cuts at: 1493 3143 3457 3683 3835 5944 1493 Size: 1650 314 226 152 2109 1697 Fragments arranged by size: 2109 1697 1650 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2616 3386 3473 1870 Size: 73 673 770 87 4545 Fragments arranged by size: 4545 770 673 87 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3469 4924 618 Size: 325 217 627 1682 1455 1842 Fragments arranged by size: 1842 1682 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2079 2135 2208 2331 2558 2702 2832 Size: 663 25 56 73 123 227 144 130 Cuts at: 2832 2858 2867 3002 3127 3243 3467 1391 Size: 26 9 135 125 116 224 4072 Fragments arranged by size: 4072 663 227 224 144 135 130 125 123 116 73 56 26 25 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BbvCI CC'TCA_GC Cuts at: 3166 3166 Size: 6148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 2118 1548 Size: 570 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2964 6143 2964 Size: 3179 2969 BlpI GC'TnA_GC Cuts at: 2103 3408 3565 2103 Size: 1305 157 4686 Fragments arranged by size: 4686 1305 157 Bme1580I G_kGCm'C Cuts at: 1790 2054 2079 2558 2702 2858 2902 3002 4209 Size: 264 25 479 144 156 44 100 1207 Cuts at: 4209 5365 1790 Size: 1156 2573 Fragments arranged by size: 2573 1207 1156 479 264 156 144 100 44 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2628 3228 3879 Size: 416 53 83 399 24 1211 600 651 Cuts at: 3879 4837 442 Size: 958 1753 Fragments arranged by size: 1753 1211 958 651 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2363 2666 2717 2783 3262 3291 4808 Size: 944 180 303 51 66 479 29 1517 Cuts at: 4808 1239 Size: 2579 Fragments arranged by size: 2579 1517 944 479 303 180 66 51 29 Bpu10I CC'TnA_GC Cuts at: 3166 3166 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3262 4811 882 Size: 617 1763 1549 2219 Fragments arranged by size: 2219 1763 1549 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3955 4337 4972 Size: 53 83 186 936 476 1946 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1946 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2475 2502 2505 2532 2894 Size: 30 165 30 1072 27 3 27 362 Cuts at: 2894 2897 2924 2927 2963 2993 3009 3039 1178 Size: 3 27 3 36 30 16 30 4287 Fragments arranged by size: 4287 1072 362 165 36 30 30 30 30 27 27 27 16 3 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2902 1186 Size: 1716 4432 BseYI C'CCAG_C Cuts at: 2124 2330 2393 2567 2825 2930 5351 5847 2124 Size: 206 63 174 258 105 2421 496 2425 Fragments arranged by size: 2425 2421 496 258 206 174 105 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 5224 Fragments arranged by size: 5224 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2902 3127 4209 4294 5365 2902 Size: 225 1082 85 1071 3685 Fragments arranged by size: 3685 1082 1071 225 85 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 BsmBI CGTCTCn'nnnn_ Cuts at: 2983 2983 Size: 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2079 2135 2208 2331 2558 2702 Size: 399 264 25 56 73 123 227 144 Cuts at: 2702 2832 2858 2867 2902 3002 3127 3243 3467 Size: 130 26 9 35 100 125 116 224 Cuts at: 3467 4209 4294 5365 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 264 227 224 144 130 125 123 116 100 85 73 56 35 26 25 9 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3228 3319 3503 844 Size: 2384 91 184 3489 Fragments arranged by size: 3489 2384 184 91 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2807 2401 Size: 406 5742 BstYI r'GATC_y Cuts at: 1972 2964 3478 3845 4230 4247 5699 5785 5797 Size: 992 514 367 385 17 1452 86 12 Cuts at: 5797 6143 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 992 514 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2410 2659 3013 2410 Size: 249 354 5545 Fragments arranged by size: 5545 354 249 BtgI C'CryG_G Cuts at: 513 1065 2872 513 Size: 552 1807 3789 Fragments arranged by size: 3789 1807 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3098 3664 4458 4478 3098 Size: 566 794 20 4768 Fragments arranged by size: 4768 794 566 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2809 3595 4484 Size: 54 1425 50 275 18 979 786 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1425 979 889 786 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4078 1353 Size: 2725 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3125 3125 Size: 6148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2363 2666 2717 2783 3262 3291 Size: 393 551 180 303 51 66 479 29 Cuts at: 3291 4211 4808 5595 5863 1239 Size: 920 597 787 268 1524 Fragments arranged by size: 1524 920 787 597 551 479 393 303 268 180 66 51 29 EcoO109I rG'GnC_Cy Cuts at: 2051 2058 2075 2076 2539 2555 2698 2699 2855 Size: 7 17 1 463 16 143 1 156 Cuts at: 2855 2999 3180 2051 Size: 144 181 5019 Fragments arranged by size: 5019 463 181 156 144 143 17 16 7 1 1 EcoRI G'AATT_C Cuts at: 3143 3457 3835 3143 Size: 314 378 5456 Fragments arranged by size: 5456 378 314 EcoRV GAT'ATC Cuts at: 2453 2453 Size: 6148 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 2806 5295 1678 Size: 1128 2489 2531 Fragments arranged by size: 2531 2489 1128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2963 2995 4789 Size: 32 135 32 669 32 690 32 1794 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1794 690 669 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3234 5262 5446 1305 Size: 1929 2028 184 2007 Fragments arranged by size: 2028 2007 1929 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2811 10 Size: 54 1425 343 979 3347 Fragments arranged by size: 3347 1425 979 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2634 2951 3353 3359 4106 4465 4624 Size: 648 1468 317 402 6 747 359 159 Cuts at: 4624 518 Size: 2042 Fragments arranged by size: 2042 1468 747 648 402 359 317 159 6 MspA1I CmG'CkG Cuts at: 1606 2124 2295 3564 4241 5389 5634 1606 Size: 518 171 1269 677 1148 245 2120 Fragments arranged by size: 2120 1269 1148 677 518 245 171 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5596 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 2592 3506 5051 1132 Size: 97 1363 914 1545 2229 Fragments arranged by size: 2229 1545 1363 914 97 PasI CC'CwG_GG Cuts at: 1348 2714 2851 1348 Size: 1366 137 4645 Fragments arranged by size: 4645 1366 137 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 2097 1108 Size: 989 5159 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2475 2507 4217 4249 5730 5762 2475 Size: 32 1710 32 1481 32 2861 Fragments arranged by size: 2861 1710 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 3180 2058 Size: 1122 5026 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PspOMI G'GGCC_C Cuts at: 2050 2075 2554 2698 2854 2998 2050 Size: 25 479 144 156 144 5200 Fragments arranged by size: 5200 479 156 144 144 25 PstI C_TGCA'G Cuts at: 830 2317 2697 3500 830 Size: 1487 380 803 3478 Fragments arranged by size: 3478 1487 803 380 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2124 2124 Size: 6148 SacI G_AGCT'C Cuts at: 3127 3127 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SbfI CC_TGCA'GG Cuts at: 2697 3500 2697 Size: 803 5345 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2313 2693 3184 3496 4631 5312 5503 Size: 219 1268 380 491 312 1135 681 191 Cuts at: 5503 826 Size: 1471 Fragments arranged by size: 1471 1268 1135 681 491 380 312 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 2618 3475 1872 Size: 746 857 4545 Fragments arranged by size: 4545 857 746 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2035 2208 3324 3587 513 Size: 552 970 173 1116 263 3074 Fragments arranged by size: 3074 1116 970 552 263 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3304 4175 4192 4345 4530 4975 325 Size: 1255 1724 871 17 153 185 445 1498 Fragments arranged by size: 1724 1498 1255 871 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2883 4394 6106 Size: 274 80 33 51 1443 906 1511 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1712 1511 1443 906 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 2894 2926 3009 3041 6108 6140 2502 Size: 32 360 32 83 32 3067 32 2510 Fragments arranged by size: 3067 2510 360 83 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2975 1316 Size: 1659 4489 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2401 2641 2848 3241 2338 Size: 63 240 207 393 5245 Fragments arranged by size: 5245 393 240 207 63 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 2616 3473 1870 Size: 746 857 4545 Fragments arranged by size: 4545 857 746 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI