(Circular) (Six-base) MAPSORT of: pFN21AB7250.seq Check: 5080 from: 1 to: 5149 pFN21AB7250 5149 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2354 2354 Size: 5149 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2959 278 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2492 1937 Size: 555 4594 Acc65I G'GTAC_C Cuts at: 2470 2470 Size: 5149 AclI AA'CG_TT Cuts at: 1563 3276 3649 1563 Size: 1713 373 3063 Fragments arranged by size: 3063 1713 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3212 4596 4864 1632 Size: 1580 1384 268 1917 Fragments arranged by size: 1917 1580 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4952 AflIII A'CryG_T Cuts at: 1203 4048 5025 1203 Size: 2845 977 1327 Fragments arranged by size: 2845 1327 977 AgeI A'CCGG_T Cuts at: 3980 3980 Size: 5149 AhdI GACnn_n'nnGTC Cuts at: 2177 3878 2177 Size: 1701 3448 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5149 AlwNI CAG_nnn'CTG Cuts at: 2320 2417 2892 4464 2320 Size: 97 475 1572 3005 Fragments arranged by size: 3005 1572 475 97 ApaI G_GGCC'C Cuts at: 2200 2279 2297 2200 Size: 79 18 5052 Fragments arranged by size: 5052 79 18 ApaLI G'TGCA_C Cuts at: 3206 4362 3206 Size: 1156 3993 ApoI r'AATT_y Cuts at: 1493 2458 2684 2836 4945 1493 Size: 965 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 226 152 AseI AT'TA_AT Cuts at: 160 1032 3703 160 Size: 872 2671 1606 Fragments arranged by size: 2671 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 AvaI C'yCGr_G Cuts at: 1870 1943 2272 2290 2474 1870 Size: 73 329 18 184 4545 Fragments arranged by size: 4545 329 184 73 18 BamHI G'GATC_C Cuts at: 2013 2479 2846 2013 Size: 466 367 4316 Fragments arranged by size: 4316 466 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2157 2216 2342 2470 3925 Size: 325 217 627 370 59 126 128 1455 Cuts at: 3925 618 Size: 1842 Fragments arranged by size: 1842 1455 627 370 325 217 128 126 59 BanII G_rGCy'C Cuts at: 1391 2121 2135 2200 2279 2297 2468 1391 Size: 730 14 65 79 18 171 4072 Fragments arranged by size: 4072 730 171 79 65 18 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 928 Size: 971 391 3787 Fragments arranged by size: 3787 971 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3340 3374 1903 Size: 34 1403 34 3678 Fragments arranged by size: 3678 1403 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3041 4251 3041 Size: 1210 3939 BclI T'GATC_A Cuts at: 1548 1548 Size: 5149 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2204 2225 3760 Size: 107 122 71 631 605 532 21 1535 Cuts at: 3760 136 Size: 1525 Fragments arranged by size: 1535 1525 631 605 532 122 107 71 21 BglII A'GATC_T Cuts at: 5144 5144 Size: 5149 BlpI GC'TnA_GC Cuts at: 2566 2566 Size: 5149 Bme1580I G_kGCm'C Cuts at: 1790 2200 2279 2297 2345 3210 4366 1790 Size: 410 79 18 48 865 1156 2573 Fragments arranged by size: 2573 1156 865 410 79 48 18 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5149 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2880 3838 442 Size: 416 53 83 399 24 1463 958 1753 Fragments arranged by size: 1753 1463 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 3809 1239 Size: 947 1623 2579 Fragments arranged by size: 2579 1623 947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3274 4139 4437 3274 Size: 865 298 3986 Fragments arranged by size: 3986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3812 882 Size: 617 2313 2219 Fragments arranged by size: 2313 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2940 493 Size: 711 1736 2702 Fragments arranged by size: 2702 1736 711 BsaBI GATnn'nnATC Cuts at: 5143 5143 Size: 5149 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2956 3338 3973 275 Size: 53 83 186 936 1423 382 635 1451 Fragments arranged by size: 1451 1423 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3582 3980 4254 4401 4812 3582 Size: 398 274 147 411 3919 Fragments arranged by size: 3919 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4924 Fragments arranged by size: 4924 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 1186 Size: 850 4299 BseYI C'CCAG_C Cuts at: 2122 2313 2338 2426 4352 4848 2122 Size: 191 25 88 1926 496 2423 Fragments arranged by size: 2423 1926 496 191 88 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 1163 Size: 573 639 3937 Fragments arranged by size: 3937 639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 2397 2401 2599 3360 4388 Size: 275 186 24 372 4 198 761 1028 Cuts at: 4388 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 372 275 198 186 24 4 BsiHKAI G_wGCw'C Cuts at: 3210 3295 4366 3210 Size: 85 1071 3993 Fragments arranged by size: 3993 1071 85 BsmI GAATG_Cn' Cuts at: 2660 2753 2660 Size: 93 5056 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2135 2200 2279 2297 2345 2468 Size: 399 331 14 65 79 18 48 123 Cuts at: 2468 3210 3295 4366 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 123 85 79 65 48 18 14 BspEI T'CCGG_A Cuts at: 4812 4812 Size: 5149 BspHI T'CATG_A Cuts at: 3038 4768 3038 Size: 1730 3419 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2354 2504 844 Size: 1510 150 3489 Fragments arranged by size: 3489 1510 150 BsrBI CCG'CTC Cuts at: 1394 1823 3036 1394 Size: 429 1213 3507 Fragments arranged by size: 3507 1213 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3644 3818 65 Size: 1183 2396 174 1396 Fragments arranged by size: 2396 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2219 2392 2396 2400 3793 3980 1700 Size: 110 409 173 4 4 1393 187 2869 Fragments arranged by size: 2869 1393 409 187 173 110 4 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5149 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5149 BssSI C'ACGA_G Cuts at: 3209 4221 3209 Size: 1012 4137 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5149 BstBI TT'CG_AA Cuts at: 3953 3953 Size: 5149 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5149 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2444 Size: 5149 BstYI r'GATC_y Cuts at: 1972 2013 2479 2846 3231 3248 4700 4786 4798 Size: 41 466 367 385 17 1452 86 12 Cuts at: 4798 5144 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 466 385 367 346 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3658 Fragments arranged by size: 3658 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4410 Fragments arranged by size: 4410 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3459 3479 2665 Size: 794 20 4335 Fragments arranged by size: 4335 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5149 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5114 DraI TTT'AAA Cuts at: 2453 2453 Size: 5149 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4156 809 Size: 507 2840 1802 Fragments arranged by size: 2840 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2260 2394 Size: 54 1425 50 275 18 192 238 134 Cuts at: 2394 2398 2596 3485 5114 8 Size: 4 198 889 1629 43 Fragments arranged by size: 1629 1425 889 275 238 198 192 134 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2022 2394 2398 2596 1537 Size: 275 210 372 4 198 4090 Fragments arranged by size: 4090 372 275 210 198 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3079 1353 Size: 1726 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3736 4110 4256 387 Size: 1064 138 2147 374 146 1280 Fragments arranged by size: 2147 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 3212 3809 4596 4864 1239 Size: 393 554 1026 597 787 268 1524 Fragments arranged by size: 1524 1026 787 597 554 393 268 EcoO109I rG'GnC_Cy Cuts at: 2092 2197 2276 2294 2304 2092 Size: 105 79 18 10 4937 Fragments arranged by size: 4937 105 79 18 10 EcoRI G'AATT_C Cuts at: 2458 2836 2458 Size: 378 4771 FseI GG_CCGG'CC Cuts at: 2400 2400 Size: 5149 FspI TGC'GCA Cuts at: 3655 3655 Size: 5149 HaeII r_GCGC'y Cuts at: 1678 4296 1678 Size: 2618 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2163 2195 3790 3822 3864 Size: 32 135 32 591 32 1595 32 42 Cuts at: 3864 3896 1373 Size: 32 2626 Fragments arranged by size: 2626 1595 591 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2493 2739 669 Size: 988 281 555 246 3079 Fragments arranged by size: 3079 988 555 281 246 HindIII A'AGCT_T Cuts at: 748 2186 748 Size: 1438 3711 HpaI GTT'AAC Cuts at: 2739 2739 Size: 5149 KpnI G_GTAC'C Cuts at: 2474 2474 Size: 5149 MfeI C'AATT_G Cuts at: 2748 2748 Size: 5149 MluI A'CGCG_T Cuts at: 5025 5025 Size: 5149 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4263 4447 1305 Size: 2958 184 2007 Fragments arranged by size: 2958 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3327 Fragments arranged by size: 3327 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 3107 3466 3625 518 Size: 648 1276 665 359 159 2042 Fragments arranged by size: 2042 1276 665 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2565 3242 4390 4635 1606 Size: 959 677 1148 245 2120 Fragments arranged by size: 2120 1148 959 677 245 NaeI GCC'GGC Cuts at: 2221 2398 2402 2221 Size: 177 4 4968 Fragments arranged by size: 4968 177 4 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3658 Fragments arranged by size: 3658 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5149 NgoMIV G'CCGG_C Cuts at: 2219 2396 2400 2219 Size: 177 4 4968 Fragments arranged by size: 4968 177 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5149 NotI GC'GGCC_GC Cuts at: 2596 2596 Size: 5149 NspI r_CATG'y Cuts at: 1132 1229 2507 4052 1132 Size: 97 1278 1545 2229 Fragments arranged by size: 2229 1545 1278 97 PasI CC'CwG_GG Cuts at: 1348 2029 2112 1348 Size: 681 83 4385 Fragments arranged by size: 4385 681 83 PciI A'CATG_T Cuts at: 4048 4048 Size: 5149 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5149 PfoI T'CCnGG_A Cuts at: 2250 2250 Size: 5149 PmeI GTTT'AAAC Cuts at: 2453 2453 Size: 5149 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3218 3250 4731 4763 3218 Size: 32 1481 32 3604 Fragments arranged by size: 3604 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2304 2092 Size: 212 4937 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5149 PsiI TTA'TAA Cuts at: 2719 2719 Size: 5149 PspOMI G'GGCC_C Cuts at: 2196 2275 2293 2196 Size: 79 18 5052 Fragments arranged by size: 5052 79 18 PstI C_TGCA'G Cuts at: 830 2058 2151 2501 830 Size: 1228 93 350 3478 Fragments arranged by size: 3478 1228 350 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5149 SalI G'TCGA_C Cuts at: 1936 2491 1936 Size: 555 4594 SanDI GG'GwC_CC Cuts at: 2092 2092 Size: 5149 SbfI CC_TGCA'GG Cuts at: 2501 2501 Size: 5149 ScaI AGT'ACT Cuts at: 3397 5109 3397 Size: 1712 3437 SfcI C'TryA_G Cuts at: 826 1045 2054 2147 2497 3632 4313 4504 826 Size: 219 1009 93 350 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 350 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5149 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5149 SmaI CCC'GGG Cuts at: 1872 2476 1872 Size: 604 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3253 4154 4416 820 Size: 197 926 1310 901 262 1553 Fragments arranged by size: 1553 1310 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2940 493 Size: 2447 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5149 SphI G_CATG'C Cuts at: 1132 2507 1132 Size: 1375 3774 SspI AAT'ATT Cuts at: 5 52 3073 5 Size: 47 3021 2081 Fragments arranged by size: 3021 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5149 StyI C'CwwG_G Cuts at: 513 1065 2004 2588 513 Size: 552 939 584 3074 Fragments arranged by size: 3074 939 584 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3176 3193 3346 3531 3976 325 Size: 1255 1596 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3395 5107 96 Size: 274 80 33 51 1443 1418 1712 138 Fragments arranged by size: 1712 1443 1418 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5109 5141 5109 Size: 32 5117 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5149 XbaI T'CTAG_A Cuts at: 2485 2485 Size: 5149 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5149 XmaI C'CCGG_G Cuts at: 1870 2474 1870 Size: 604 4545 XmnI GAAnn'nnTTC Cuts at: 3278 3278 Size: 5149 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2957 276 Size: 53 83 186 936 1423 2468 Fragments arranged by size: 2468 1423 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI