Restriction map for
pFN21AB7248
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BsrDI BssHII Bst1107I
BstZ17I Bsu36I CelII ClaI DraI
DsaI Eam1105I Eco81I EcoRI EcoT22I
FbaI HincII HindII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AcsI 997 GAATTT [GA]^AATT[CT]
Alw44I 97 GTGCAC G^TGCAC
Aor51HI 759 AGCGCT AGC^GCT
ApaLI 97 GTGCAC G^TGCAC
ApoI 997 GAATTT [GA]^AATT[CT]
Asp700I 87 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 438 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 308 TGCGCA TGC^GCA
BalI 598 TGGCCA TGG^CCA
BanI 72 GGCGCC G^G[CT][GA]CC
BbeI 72 GGCGCC GGCGC^C
BbsI 1153 GAAGAC GAAGAC(2/6)
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BcgI 1494 CGAAAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1050 AGATCT A^GATCT
BpuAI 1153 GAAGAC GAAGAC(2/6)
BsaI 1507 GAGACC GGTCTC(1/5)
BsgI 1502 GTGCAG GTGCAG(16/14)
BsiEI 1237 CGGCCG CG[GA][CT]^CG
BsmI 670 GCATTC GAATGC(1/-1)
BspHI 143 TCATGA T^CATGA
BspLU11I 1157 ACATGT A^CATGT
BsrBI 1179 GAGCGG CCGCTC(-3/-3)
BstBI 1492 TTCGAA TT^CGAA
BstEII 1315 GGTGACC G^GT[ACGT]ACC
BstPI 1315 GGTGACC G^GT[ACGT]ACC
BstXI 1259 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1050 AGATCT [GA]^GATC[CT]
CpoI 164 CGGTCCG CG^G[AT]CCG
EagI 1237 CGGCCG C^GGCCG
EclXI 1237 CGGCCG C^GGCCG
Eco47III 759 AGCGCT AGC^GCT
Eco52I 1237 CGGCCG C^GGCCG
EcoO65I 1315 GGTGACC G^GT[ACGT]ACC
EcoRV 710 GATATC GAT^ATC
EcoT14I 902 CCTTGG C^C[AT][AT]GG
FseI 1238 GGCCGGCC GGCCGG^CC
FspI 308 TGCGCA TGC^GCA
HindIII 1039 AAGCTT A^AGCTT
KasI 72 GGCGCC G^GCGCC
MflI 1050 AGATCT [GA]^GATC[CT]
MluNI 598 TGGCCA TGG^CCA
MscI 598 TGGCCA TGG^CCA
NarI 72 GGCGCC GG^CGCC
NspI 1157 ACATGT [GA]CATG^[CT]
NspV 1492 TTCGAA TT^CGAA
PflFI 438 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 887 GAGTC GAGTC(4/5)
PstI 121 CTGCAG CTGCA^G
RcaI 143 TCATGA T^CATGA
RsrII 164 CGGTCCG CG^G[AT]CCG
SacI 1357 GAGCTC GAGCT^C
SapI 156 GCTCTTC GCTCTTC(1/4)
SexAI 663 ACCTGGT A^CC[AT]GGT
SfcI 121 CTGCAG C^T[GA][CT]AG
SfiI 129 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 72 GGCGCC GGC^GCC
SfuI 1492 TTCGAA TT^CGAA
SgrAI 324 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1333 CTCAAG C^T[CT][GA]AG
StuI 899 AGGCCT AGG^CCT
StyI 902 CCTTGG C^C[AT][AT]GG
TfiI 541 GATTC G^A[AT]TC
Tth111I 438 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 188 CCATGATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1050 AGATCT [GA]^GATC[CT]
XmnI 87 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 396 CGCG CG^CG
861 CGCG
AcyI 72 GGCGCC G[GA]^CG[CT]C
418 GACGCC
AflIII 926 ACACGT A^C[GA][CT]GT
1157 ACATGT
AlwI 498 GATCC GGATC(4/5)
1479 GGATC
AspHI 97 GTGCAC G[AT]GC[AT]^C
1357 GAGCTC
BanII 231 GGGCTC G[GA]GC[CT]^C
1357 GAGCTC
BglI 75 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
130 GCCCAGGTGGC
BsaHI 72 GGCGCC G[GA]^CG[CT]C
418 GACGCC
BsiHKAI 97 GTGCAC G[AT]GC[AT]^C
1357 GAGCTC
BsmBI 970 CGTCTC CGTCTC(1/5)
573 GAGACG
BsmFI 525 GTCCC GGGAC(10/14)
1132 GTCCC
Bsp1407I 631 TGTACA T^GTACA
827 TGTACA
BsrGI 631 TGTACA T^GTACA
827 TGTACA
BsrI 723 ACTGG ACTGG(1/-1)
501 CCAGT
BssSI 100 CACGAG CACGAG(-5/-1)
1009 CTCGTG
BstAPI 99 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
360 GCAGGCGCTGC
BstUI 396 CGCG CG^CG
861 CGCG
DrdI 962 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1297 GACGGCAGCGTC
EarI 157 CTCTTC CTCTTC(1/4)
849 GAAGAG
EcoNI 120 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
291 CCTGCTGAAGG
Hin1I 72 GGCGCC G[GA]^CG[CT]C
418 GACGCC
HinfI 541 GATTC G^A[ACGT]TC
887 GAGTC
Ksp632I 157 CTCTTC CTCTTC(1/4)
849 GAAGAG
MvnI 396 CGCG CG^CG
861 CGCG
PpuMI 640 AGGACCT [GA]G^G[AT]CC[CT]
750 AGGACCT
PvuII 943 CAGCTG CAG^CTG
1264 CAGCTG
SmaI 23 CCCGGG CCC^GGG
78 CCCGGG
SspBI 631 TGTACA T^GTACA
827 TGTACA
Tsp509I 651 AATT ^AATT
998 AATT
TspRI 149 CACTG CA[GC]TG[ACGT][ACGT]^
1166 CACTG
XmaCI 23 CCCGGG C^CCGGG
78 CCCGGG
XmaI 23 CCCGGG C^CCGGG
78 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TthHB8I Van91I