(Circular) (Six-base) MAPSORT of: pFN21AB7248.seq Check: 3598 from: 1 to: 6307 pFN21AB7248 6307 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3529 3529 Size: 6307 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4117 278 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3650 1937 Size: 1713 4594 Acc65I G'GTAC_C Cuts at: 3628 3628 Size: 6307 AclI AA'CG_TT Cuts at: 1563 4434 4807 1563 Size: 2871 373 3063 Fragments arranged by size: 3063 2871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 4370 5754 6022 1632 Size: 689 2049 1384 268 1917 Fragments arranged by size: 2049 1917 1384 689 268 AfeI AGC'GCT Cuts at: 2766 2766 Size: 6307 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6110 AflIII A'CryG_T Cuts at: 1203 2931 3162 5206 6183 1203 Size: 1728 231 2044 977 1327 Fragments arranged by size: 2044 1728 1327 977 231 AgeI A'CCGG_T Cuts at: 5138 5138 Size: 6307 AhdI GACnn_n'nnGTC Cuts at: 5036 5036 Size: 6307 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6307 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6307 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2804 2836 2804 Size: 32 6275 AlwNI CAG_nnn'CTG Cuts at: 2371 2649 3575 4050 5622 2371 Size: 278 926 475 1572 3056 Fragments arranged by size: 3056 1572 926 475 278 ApaLI G'TGCA_C Cuts at: 2102 4364 5520 2102 Size: 2262 1156 2889 Fragments arranged by size: 2889 2262 1156 ApoI r'AATT_y Cuts at: 1493 3002 3616 3842 3994 6103 1493 Size: 1509 614 226 152 2109 1697 Fragments arranged by size: 2109 1697 1509 614 226 152 AseI AT'TA_AT Cuts at: 160 1032 4861 160 Size: 872 3829 1606 Fragments arranged by size: 3829 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2083 2255 3026 3632 1870 Size: 73 85 55 172 771 606 4545 Fragments arranged by size: 4545 771 606 172 85 73 55 BamHI G'GATC_C Cuts at: 3637 4004 3637 Size: 367 5940 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3628 5083 618 Size: 325 217 627 290 1551 1455 1842 Fragments arranged by size: 1842 1551 1455 627 325 290 217 BanII G_rGCy'C Cuts at: 1391 2240 3366 3626 1391 Size: 849 1126 260 4072 Fragments arranged by size: 4072 1126 849 260 BbeI G_GCGC'C Cuts at: 2081 2081 Size: 6307 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 928 Size: 971 1266 4070 Fragments arranged by size: 4070 1266 971 BbvCI CC'TCA_GC Cuts at: 2117 2117 Size: 6307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3488 3522 4498 4532 1903 Size: 34 1551 34 976 34 3678 Fragments arranged by size: 3678 1551 976 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4199 5409 4199 Size: 1210 5097 BclI T'GATC_A Cuts at: 1548 1548 Size: 6307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2086 2141 4918 Size: 107 122 71 631 605 414 55 2777 Cuts at: 4918 136 Size: 1525 Fragments arranged by size: 2777 1525 631 605 414 122 107 71 55 BglII A'GATC_T Cuts at: 3055 6302 3055 Size: 3247 3060 BlpI GC'TnA_GC Cuts at: 3724 3724 Size: 6307 Bme1580I G_kGCm'C Cuts at: 1790 2106 4368 5524 1790 Size: 316 2262 1156 2573 Fragments arranged by size: 2573 2262 1156 316 BmgBI CAC'GTC Cuts at: 1318 2748 1318 Size: 1430 4877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4038 4996 442 Size: 416 53 83 399 24 2621 958 1753 Fragments arranged by size: 2621 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6307 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2706 2781 4967 1239 Size: 790 677 75 2186 2579 Fragments arranged by size: 2579 2186 790 677 75 Bpu10I CC'TnA_GC Cuts at: 2117 2117 Size: 6307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3323 4432 5297 5595 3323 Size: 1109 865 298 4035 Fragments arranged by size: 4035 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3506 4970 882 Size: 617 2007 1464 2219 Fragments arranged by size: 2219 2007 1464 617 BsaAI yAC'GTr Cuts at: 493 1204 4098 493 Size: 711 2894 2702 Fragments arranged by size: 2894 2702 711 BsaBI GATnn'nnATC Cuts at: 6301 6301 Size: 6307 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2424 4114 4496 Size: 53 83 186 936 545 346 1690 382 Cuts at: 4496 5131 275 Size: 635 1451 Fragments arranged by size: 1690 1451 936 635 545 382 346 186 83 53 BsaWI w'CCGG_w Cuts at: 4740 5138 5412 5559 5970 4740 Size: 398 274 147 411 5077 Fragments arranged by size: 5077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2679 2709 1178 Size: 30 165 30 1276 30 4776 Fragments arranged by size: 4776 1276 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2861 2864 3108 3416 1186 Size: 1675 3 244 308 4077 Fragments arranged by size: 4077 1675 308 244 3 BseYI C'CCAG_C Cuts at: 3575 5510 6006 3575 Size: 1935 496 3876 Fragments arranged by size: 3876 1935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3528 1163 Size: 573 1792 3942 Fragments arranged by size: 3942 1792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3245 3757 4518 5546 1540 Size: 275 186 1244 512 761 1028 2301 Fragments arranged by size: 2301 1244 1028 761 512 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 3366 4368 4453 5524 2106 Size: 1260 1002 85 1071 2889 Fragments arranged by size: 2889 1260 1071 1002 85 BsmI GAATG_Cn' Cuts at: 2675 3818 3911 2675 Size: 1143 93 5071 Fragments arranged by size: 5071 1143 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2572 2981 2572 Size: 409 5898 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2240 3366 3626 4368 4453 5524 Size: 399 316 134 1126 260 742 85 1071 Cuts at: 5524 1391 Size: 2174 Fragments arranged by size: 2174 1126 1071 742 399 316 260 134 85 BspEI T'CCGG_A Cuts at: 5970 5970 Size: 6307 BspHI T'CATG_A Cuts at: 2148 4196 5926 2148 Size: 2048 1730 2529 Fragments arranged by size: 2529 2048 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 3213 3529 3662 844 Size: 1813 556 316 133 3489 Fragments arranged by size: 3489 1813 556 316 133 BsrBI CCG'CTC Cuts at: 1394 1823 3186 4194 1394 Size: 429 1363 1008 3507 Fragments arranged by size: 3507 1363 1008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4802 4976 65 Size: 1183 3554 174 1396 Fragments arranged by size: 3554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2330 3240 3244 4951 5138 1700 Size: 110 520 910 4 1707 187 2869 Fragments arranged by size: 2869 1707 910 520 187 110 4 BsrGI T'GTAC_A Cuts at: 96 2636 2832 96 Size: 2540 196 3571 Fragments arranged by size: 3571 2540 196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6307 BssSI C'ACGA_G Cuts at: 2105 3014 4367 5379 2105 Size: 909 1353 1012 3033 Fragments arranged by size: 3033 1353 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 2110 2371 794 Size: 1316 261 4730 Fragments arranged by size: 4730 1316 261 BstBI TT'CG_AA Cuts at: 3498 5111 3498 Size: 1613 4694 BstEII G'GTnAC_C Cuts at: 1184 3320 1184 Size: 2136 4171 BstXI CCAn_nnnn'nTGG Cuts at: 3271 3271 Size: 6307 BstYI r'GATC_y Cuts at: 1972 3055 3637 4004 4389 4406 5858 5944 5956 Size: 1083 582 367 385 17 1452 86 12 Cuts at: 5956 6302 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1083 582 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5755 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5568 Fragments arranged by size: 5568 683 56 BtsI GCAGTG_nn' Cuts at: 3823 4617 4637 3823 Size: 794 20 5493 Fragments arranged by size: 5493 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6272 DraI TTT'AAA Cuts at: 3611 3611 Size: 6307 DraIII CAC_nnn'GTG Cuts at: 2322 2347 2790 2322 Size: 25 443 5839 Fragments arranged by size: 5839 443 25 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2973 3308 5314 809 Size: 507 1657 335 2006 1802 Fragments arranged by size: 2006 1802 1657 507 335 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2385 2603 Size: 54 1425 50 275 18 519 36 218 Cuts at: 2603 2898 3242 3754 4643 6272 8 Size: 295 344 512 889 1629 43 Fragments arranged by size: 1629 1425 889 519 512 344 295 275 218 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3242 3754 1537 Size: 275 1430 512 4090 Fragments arranged by size: 4090 1430 512 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 2849 4237 1353 Size: 815 681 1388 3423 Fragments arranged by size: 3423 1388 815 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2104 2975 3595 4894 5268 5414 Size: 1064 138 515 871 620 1299 374 146 Cuts at: 5414 387 Size: 1280 Fragments arranged by size: 1299 1280 1064 871 620 515 374 146 138 EcoICRI GAG'CTC Cuts at: 3364 3364 Size: 6307 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2321 2706 2781 4370 4967 5754 Size: 393 397 292 385 75 1589 597 787 Cuts at: 5754 6022 1239 Size: 268 1524 Fragments arranged by size: 1589 1524 787 597 397 393 385 292 268 75 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2300 2129 Size: 171 6136 EcoO109I rG'GnC_Cy Cuts at: 2646 2756 3022 2646 Size: 110 266 5931 Fragments arranged by size: 5931 266 110 EcoRI G'AATT_C Cuts at: 3616 3994 3616 Size: 378 5929 EcoRV GAT'ATC Cuts at: 2717 2717 Size: 6307 FseI GG_CCGG'CC Cuts at: 3248 3248 Size: 6307 FspI TGC'GCA Cuts at: 2315 4813 2315 Size: 2498 3809 FspAI rTGC'GCAy Cuts at: 2315 2315 Size: 6307 HaeII r_GCGC'y Cuts at: 1678 2081 2124 2372 2693 2768 5454 1678 Size: 403 43 248 321 75 2686 2531 Fragments arranged by size: 2686 2531 403 321 248 75 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2679 2711 3053 3085 4948 Size: 32 135 32 1107 32 342 32 1863 Cuts at: 4948 4980 5022 5054 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1863 1107 342 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3651 3897 669 Size: 988 281 1713 246 3079 Fragments arranged by size: 3079 1713 988 281 246 HindIII A'AGCT_T Cuts at: 748 3044 748 Size: 2296 4011 HpaI GTT'AAC Cuts at: 3897 3897 Size: 6307 KasI G'GCGC_C Cuts at: 2077 2077 Size: 6307 KpnI G_GTAC'C Cuts at: 3632 3632 Size: 6307 MfeI C'AATT_G Cuts at: 3906 3906 Size: 6307 MluI A'CGCG_T Cuts at: 6183 6183 Size: 6307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2616 5421 5605 1305 Size: 1311 2805 184 2007 Fragments arranged by size: 2805 2007 1311 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2605 10 Size: 54 1425 343 773 3712 Fragments arranged by size: 3712 1425 773 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2444 2459 2746 4265 4624 4783 Size: 648 1266 12 15 287 1519 359 159 Cuts at: 4783 518 Size: 2042 Fragments arranged by size: 2042 1519 1266 648 359 287 159 15 12 MspA1I CmG'CkG Cuts at: 1606 2189 2950 2984 3192 3271 3723 4400 5548 Size: 583 761 34 208 79 452 677 1148 Cuts at: 5548 5793 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 761 677 583 452 245 208 79 34 NaeI GCC'GGC Cuts at: 2332 3242 3246 2332 Size: 910 4 5393 Fragments arranged by size: 5393 910 4 NarI GG'CG_CC Cuts at: 2078 2078 Size: 6307 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5755 NdeI CA'TA_TG Cuts at: 387 387 Size: 6307 NgoMIV G'CCGG_C Cuts at: 2330 3240 3244 2330 Size: 910 4 5393 Fragments arranged by size: 5393 910 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6307 NotI GC'GGCC_GC Cuts at: 3754 3754 Size: 6307 NspI r_CATG'y Cuts at: 1132 1229 3166 3665 5210 1132 Size: 97 1937 499 1545 2229 Fragments arranged by size: 2229 1937 1545 499 97 PasI CC'CwG_GG Cuts at: 1348 2233 3349 1348 Size: 885 1116 4306 Fragments arranged by size: 4306 1116 885 PciI A'CATG_T Cuts at: 3162 5206 3162 Size: 2044 4263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 2704 2742 1108 Size: 1239 357 38 4673 Fragments arranged by size: 4673 1239 357 38 PmeI GTTT'AAAC Cuts at: 3611 3611 Size: 6307 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4376 4408 5889 5921 4376 Size: 32 1481 32 4762 Fragments arranged by size: 4762 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2646 2756 2646 Size: 110 6197 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6307 PsiI TTA'TAA Cuts at: 3877 3877 Size: 6307 PstI C_TGCA'G Cuts at: 830 2130 3659 830 Size: 1300 1529 3478 Fragments arranged by size: 3478 1529 1300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6307 PvuII CAG'CTG Cuts at: 2950 3271 2950 Size: 321 5986 RsrII CG'GwC_CG Cuts at: 2170 2170 Size: 6307 SacI G_AGCT'C Cuts at: 3366 3366 Size: 6307 SalI G'TCGA_C Cuts at: 1936 3649 1936 Size: 1713 4594 SapI GCTCTTCn'nnn_ Cuts at: 2168 2168 Size: 6307 SbfI CC_TGCA'GG Cuts at: 3659 3659 Size: 6307 ScaI AGT'ACT Cuts at: 4555 6267 4555 Size: 1712 4595 SexAI A'CCwGG_T Cuts at: 2668 2668 Size: 6307 SfcI C'TryA_G Cuts at: 826 1045 2126 3655 4790 5471 5662 826 Size: 219 1081 1529 1135 681 191 1471 Fragments arranged by size: 1529 1471 1135 1081 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2141 2141 Size: 6307 SfoI GGC'GCC Cuts at: 2079 2079 Size: 6307 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6307 SgrAI Cr'CCGG_yG Cuts at: 1700 2330 1700 Size: 630 5677 SmaI CCC'GGG Cuts at: 1872 2030 2085 3634 1872 Size: 158 55 1549 4545 Fragments arranged by size: 4545 1549 158 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 3338 4411 5312 5574 820 Size: 197 926 1395 1073 901 262 1553 Fragments arranged by size: 1553 1395 1073 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4098 493 Size: 3605 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6307 SphI G_CATG'C Cuts at: 1132 3665 1132 Size: 2533 3774 SspI AAT'ATT Cuts at: 5 52 4231 5 Size: 47 4179 2081 Fragments arranged by size: 4179 2081 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 4918 StyI C'CwwG_G Cuts at: 513 1065 2907 3746 513 Size: 552 1842 839 3074 Fragments arranged by size: 3074 1842 839 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2900 3389 4334 4351 4504 4689 5134 Size: 1255 1320 489 945 17 153 185 445 Cuts at: 5134 325 Size: 1498 Fragments arranged by size: 1498 1320 1255 945 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2636 2832 4553 Size: 274 80 33 51 1443 659 196 1721 Cuts at: 4553 6265 96 Size: 1712 138 Fragments arranged by size: 1721 1712 1443 659 274 196 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 6267 6299 2762 Size: 32 3473 32 2770 Fragments arranged by size: 3473 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2446 1316 Size: 1130 5177 XbaI T'CTAG_A Cuts at: 3643 3643 Size: 6307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2200 2200 Size: 6307 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6307 XmaI C'CCGG_G Cuts at: 1870 2028 2083 3632 1870 Size: 158 55 1549 4545 Fragments arranged by size: 4545 1549 158 55 XmnI GAAnn'nnTTC Cuts at: 2096 4436 2096 Size: 2340 3967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4115 276 Size: 53 83 186 936 2581 2468 Fragments arranged by size: 2581 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I FalI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacII SanDI SrfI SwaI