Restriction map for
pFN21AB7248
(Linear) (Six-base) MAPSORT of: KIBB7248.seq Check: 8941 from: 1 to: 1602
KIBB7248.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1524 1602
Size: 1524 78
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1602
Size: 316 1286
AfeI AGC'GCT
Cuts at: 0 761 1602
Size: 761 841
AflIII A'CryG_T
Cuts at: 0 926 1157 1602
Size: 926 231 445
Fragments arranged by size:
926 445 231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 799 831 1602
Size: 799 32 771
Fragments arranged by size:
799 771 32
AlwNI CAG_nnn'CTG
Cuts at: 0 366 644 1570 1602
Size: 366 278 926 32
Fragments arranged by size:
926 366 278 32
ApaLI G'TGCA_C
Cuts at: 0 97 1602
Size: 97 1505
ApoI r'AATT_y
Cuts at: 0 997 1602
Size: 997 605
AvaI C'yCGr_G
Cuts at: 0 23 78 250 1021 1602
Size: 23 55 172 771 581
Fragments arranged by size:
771 581 172 55 23
BanI G'GyrC_C
Cuts at: 0 72 1602
Size: 72 1530
BanII G_rGCy'C
Cuts at: 0 235 1361 1602
Size: 235 1126 241
Fragments arranged by size:
1126 241 235
BbeI G_GCGC'C
Cuts at: 0 76 1602
Size: 76 1526
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1602
Size: 1160 442
BbvCI CC'TCA_GC
Cuts at: 0 112 1602
Size: 112 1490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1483 1517 1602
Size: 1483 34 85
Fragments arranged by size:
1483 85 34
BglI GCCn_nnn'nGGC
Cuts at: 0 81 136 1602
Size: 81 55 1466
Fragments arranged by size:
1466 81 55
BglII A'GATC_T
Cuts at: 0 1050 1602
Size: 1050 552
Bme1580I G_kGCm'C
Cuts at: 0 101 1602
Size: 101 1501
BmgBI CAC'GTC
Cuts at: 0 743 1602
Size: 743 859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 701 776 1602
Size: 24 677 75 826
Fragments arranged by size:
826 677 75 24
Bpu10I CC'TnA_GC
Cuts at: 0 112 1602
Size: 112 1490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1602
Size: 1318 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 1501 1602
Size: 1501 101
BsaHI Gr'CG_yC
Cuts at: 0 73 419 1602
Size: 73 346 1183
Fragments arranged by size:
1183 346 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 674 704 1602
Size: 674 30 898
Fragments arranged by size:
898 674 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 856 859 1103 1411 1602
Size: 856 3 244 308 191
Fragments arranged by size:
856 308 244 191 3
BseYI C'CCAG_C
Cuts at: 0 1570 1602
Size: 1570 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 1602
Size: 1523 79
BsiEI CG_ry'CG
Cuts at: 0 1240 1602
Size: 1240 362
BsiHKAI G_wGCw'C
Cuts at: 0 101 1361 1602
Size: 101 1260 241
Fragments arranged by size:
1260 241 101
BsmI GAATG_Cn'
Cuts at: 0 670 1602
Size: 670 932
BsmBI CGTCTCn'nnnn_
Cuts at: 0 567 976 1602
Size: 567 409 626
Fragments arranged by size:
626 567 409
Bsp1286I G_dGCh'C
Cuts at: 0 101 235 1361 1602
Size: 101 134 1126 241
Fragments arranged by size:
1126 241 134 101
BspHI T'CATG_A
Cuts at: 0 143 1602
Size: 143 1459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 652 1208 1524 1602
Size: 652 556 316 78
Fragments arranged by size:
652 556 316 78
BsrBI CCG'CTC
Cuts at: 0 1181 1602
Size: 1181 421
BsrFI r'CCGG_y
Cuts at: 0 325 1235 1239 1602
Size: 325 910 4 363
Fragments arranged by size:
910 363 325 4
BsrGI T'GTAC_A
Cuts at: 0 631 827 1602
Size: 631 196 775
Fragments arranged by size:
775 631 196
BssSI C'ACGA_G
Cuts at: 0 100 1009 1602
Size: 100 909 593
Fragments arranged by size:
909 593 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 366 1602
Size: 105 261 1236
Fragments arranged by size:
1236 261 105
BstBI TT'CG_AA
Cuts at: 0 1493 1602
Size: 1493 109
BstEII G'GTnAC_C
Cuts at: 0 1315 1602
Size: 1315 287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1266 1602
Size: 1266 336
BstYI r'GATC_y
Cuts at: 0 1050 1602
Size: 1050 552
DraIII CAC_nnn'GTG
Cuts at: 0 317 342 785 1602
Size: 317 25 443 817
Fragments arranged by size:
817 443 317 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 968 1303 1602
Size: 968 335 299
Fragments arranged by size:
968 335 299
EaeI y'GGCC_r
Cuts at: 0 344 380 598 893 1237 1602
Size: 344 36 218 295 344 365
Fragments arranged by size:
365 344 344 295 218 36
EagI C'GGCC_G
Cuts at: 0 1237 1602
Size: 1237 365
EarI CTCTTCn'nnn_
Cuts at: 0 163 844 1602
Size: 163 681 758
Fragments arranged by size:
758 681 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 99 970 1590 1602
Size: 99 871 620 12
Fragments arranged by size:
871 620 99 12
EcoICRI GAG'CTC
Cuts at: 0 1359 1602
Size: 1359 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 316 701 776 1602
Size: 24 292 385 75 826
Fragments arranged by size:
826 385 292 75 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 295 1602
Size: 124 171 1307
Fragments arranged by size:
1307 171 124
EcoO109I rG'GnC_Cy
Cuts at: 0 641 751 1017 1602
Size: 641 110 266 585
Fragments arranged by size:
641 585 266 110
EcoRV GAT'ATC
Cuts at: 0 712 1602
Size: 712 890
FseI GG_CCGG'CC
Cuts at: 0 1243 1602
Size: 1243 359
FspI TGC'GCA
Cuts at: 0 310 1602
Size: 310 1292
FspAI rTGC'GCAy
Cuts at: 0 310 1602
Size: 310 1292
HaeII r_GCGC'y
Cuts at: 0 76 119 367 688 763 1602
Size: 76 43 248 321 75 839
Fragments arranged by size:
839 321 248 76 75 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 674 706 1048 1080 1602
Size: 674 32 342 32 522
Fragments arranged by size:
674 522 342 32 32
HindIII A'AGCT_T
Cuts at: 0 1039 1602
Size: 1039 563
KasI G'GCGC_C
Cuts at: 0 72 1602
Size: 72 1530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1602
Size: 611 991
MscI TGG'CCA
Cuts at: 0 600 1602
Size: 600 1002
MslI CAynn'nnrTG
Cuts at: 0 427 439 454 741 1602
Size: 427 12 15 287 861
Fragments arranged by size:
861 427 287 15 12
MspA1I CmG'CkG
Cuts at: 0 184 945 979 1187 1266 1602
Size: 184 761 34 208 79 336
Fragments arranged by size:
761 336 208 184 79 34
NaeI GCC'GGC
Cuts at: 0 327 1237 1241 1602
Size: 327 910 4 361
Fragments arranged by size:
910 361 327 4
NarI GG'CG_CC
Cuts at: 0 73 1602
Size: 73 1529
NgoMIV G'CCGG_C
Cuts at: 0 325 1235 1239 1602
Size: 325 910 4 363
Fragments arranged by size:
910 363 325 4
NspI r_CATG'y
Cuts at: 0 1161 1602
Size: 1161 441
PasI CC'CwG_GG
Cuts at: 0 228 1344 1602
Size: 228 1116 258
Fragments arranged by size:
1116 258 228
PciI A'CATG_T
Cuts at: 0 1157 1602
Size: 1157 445
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 699 737 1602
Size: 342 357 38 865
Fragments arranged by size:
865 357 342 38
PpuMI rG'GwC_Cy
Cuts at: 0 641 751 1602
Size: 641 110 851
Fragments arranged by size:
851 641 110
PstI C_TGCA'G
Cuts at: 0 125 1602
Size: 125 1477
PvuII CAG'CTG
Cuts at: 0 945 1266 1602
Size: 945 321 336
Fragments arranged by size:
945 336 321
RsrII CG'GwC_CG
Cuts at: 0 165 1602
Size: 165 1437
SacI G_AGCT'C
Cuts at: 0 1361 1602
Size: 1361 241
SapI GCTCTTCn'nnn_
Cuts at: 0 163 1602
Size: 163 1439
SexAI A'CCwGG_T
Cuts at: 0 663 1602
Size: 663 939
SfcI C'TryA_G
Cuts at: 0 121 1602
Size: 121 1481
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 136 1602
Size: 136 1466
SfoI GGC'GCC
Cuts at: 0 74 1602
Size: 74 1528
SgrAI Cr'CCGG_yG
Cuts at: 0 325 1602
Size: 325 1277
SmaI CCC'GGG
Cuts at: 0 25 80 1602
Size: 25 55 1522
Fragments arranged by size:
1522 55 25
SmlI C'TyrA_G
Cuts at: 0 1333 1602
Size: 1333 269
StuI AGG'CCT
Cuts at: 0 901 1602
Size: 901 701
StyI C'CwwG_G
Cuts at: 0 902 1602
Size: 902 700
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 1384 1602
Size: 895 489 218
Fragments arranged by size:
895 489 218
TatI w'GTAC_w
Cuts at: 0 631 827 1602
Size: 631 196 775
Fragments arranged by size:
775 631 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 1602
Size: 757 32 813
Fragments arranged by size:
813 757 32
Tth111I GACn'n_nGTC
Cuts at: 0 441 1602
Size: 441 1161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 1602
Size: 195 1407
XmaI C'CCGG_G
Cuts at: 0 23 78 1602
Size: 23 55 1524
Fragments arranged by size:
1524 55 23
XmnI GAAnn'nnTTC
Cuts at: 0 91 1602
Size: 91 1511
Enzymes that do cut:
AarI AcuI AfeI AflIII AloI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PpuMI PstI PvuII RsrII SacI SapI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaAI
BsaBI BsaWI BsiWI BspEI BsrDI BssHII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI EcoRI FalI
HincII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI SacII SalI
SanDI SbfI ScaI SgfI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI