(Circular) (Six-base) MAPSORT of: pFN21AB7246.seq Check: 1489 from: 1 to: 6265 pFN21AB7246 6265 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2077 2095 2312 2821 2921 3542 3566 Size: 391 54 18 217 509 100 621 24 Cuts at: 3566 4328 5712 5980 1632 Size: 762 1384 268 1917 Fragments arranged by size: 1917 1384 762 621 509 391 268 217 100 54 24 18 AfeI AGC'GCT Cuts at: 2044 3325 3496 2044 Size: 1281 171 4813 Fragments arranged by size: 4813 1281 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2793 5164 6141 1203 Size: 1590 2371 977 1327 Fragments arranged by size: 2371 1590 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 2625 2798 1166 Size: 1459 173 4633 Fragments arranged by size: 4633 1459 173 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2278 2476 3019 3169 3199 4008 5580 2278 Size: 198 543 150 30 809 1572 2963 Fragments arranged by size: 2963 1572 809 543 198 150 30 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 6265 ApaLI G'TGCA_C Cuts at: 3286 4322 5478 3286 Size: 1036 1156 4073 Fragments arranged by size: 4073 1156 1036 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 3590 1870 Size: 73 1647 4545 Fragments arranged by size: 4545 1647 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 3586 5041 618 Size: 325 217 627 355 1444 1455 1842 Fragments arranged by size: 1842 1455 1444 627 355 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2229 2551 3449 3584 1391 Size: 638 200 322 898 135 4072 Fragments arranged by size: 4072 898 638 322 200 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3533 3554 928 Size: 971 1634 21 3639 Fragments arranged by size: 3639 1634 971 21 BbvCI CC'TCA_GC Cuts at: 2898 2904 2898 Size: 6 6259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2151 2472 4876 Size: 107 122 71 631 605 479 321 2404 Cuts at: 4876 136 Size: 1525 Fragments arranged by size: 2404 1525 631 605 479 321 122 107 71 BglII A'GATC_T Cuts at: 2988 6260 2988 Size: 3272 2993 BlpI GC'TnA_GC Cuts at: 2050 3084 3682 2050 Size: 1034 598 4633 Fragments arranged by size: 4633 1034 598 Bme1580I G_kGCm'C Cuts at: 1790 2029 2240 3290 4326 5482 1790 Size: 239 211 1050 1036 1156 2573 Fragments arranged by size: 2573 1156 1050 1036 239 211 BmgBI CAC'GTC Cuts at: 1318 2796 1318 Size: 1478 4787 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2305 3996 4954 Size: 416 53 83 399 24 888 1691 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 1753 1691 958 888 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2747 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2441 2498 2516 2543 2732 2759 3158 Size: 1181 21 57 18 27 189 27 399 Cuts at: 3158 3275 3357 3444 3475 3487 4925 1239 Size: 117 82 87 31 12 1438 2579 Fragments arranged by size: 2579 1438 1181 399 189 117 87 82 57 31 27 27 21 18 12 Bpu10I CC'TnA_GC Cuts at: 2510 2684 2898 2904 3117 2510 Size: 174 214 6 213 5658 Fragments arranged by size: 5658 214 213 174 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2053 2642 2657 3026 3092 4390 5255 5553 2053 Size: 589 15 369 66 1298 865 298 2765 Fragments arranged by size: 2765 1298 865 589 369 298 66 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 4928 882 Size: 617 831 2598 2219 Fragments arranged by size: 2598 2219 831 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 2300 6259 2300 Size: 3959 2306 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 2576 2582 2606 Size: 30 165 30 1011 30 132 6 24 Cuts at: 2606 2612 3352 3382 1178 Size: 6 740 30 4061 Fragments arranged by size: 4061 1011 740 165 132 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 2603 2624 2627 2888 3278 3298 3354 Size: 1414 3 21 3 261 390 20 56 Cuts at: 3354 1186 Size: 4097 Fragments arranged by size: 4097 1414 390 261 56 21 20 3 3 BseYI C'CCAG_C Cuts at: 2188 2764 2949 5468 5964 2188 Size: 576 185 2519 496 2489 Fragments arranged by size: 2519 2489 576 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3289 3391 1163 Size: 573 1553 102 4037 Fragments arranged by size: 4037 1553 573 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3209 3290 3365 4326 4411 5482 3209 Size: 81 75 961 85 1071 3992 Fragments arranged by size: 3992 1071 961 85 81 75 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 2417 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2229 2240 2551 3209 3290 3365 Size: 399 239 200 11 311 658 81 75 Cuts at: 3365 3449 3584 4326 4411 5482 1391 Size: 84 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 399 311 239 200 135 85 84 81 75 11 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 2157 4325 5337 2157 Size: 2168 1012 3085 Fragments arranged by size: 3085 2168 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 794 Size: 1646 4619 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2957 2957 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2988 3595 3962 4347 4364 5816 5902 5914 Size: 1016 607 367 385 17 1452 86 12 Cuts at: 5914 6260 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1016 607 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2811 513 Size: 552 1746 3967 Fragments arranged by size: 3967 1746 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 3781 4575 4595 3781 Size: 794 20 5451 Fragments arranged by size: 5451 794 20 ClaI AT'CG_AT Cuts at: 1554 2304 1554 Size: 750 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2651 3535 5272 809 Size: 507 1335 884 1737 1802 Fragments arranged by size: 1802 1737 1335 884 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3156 3712 4601 Size: 54 1425 50 275 18 1326 556 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1326 889 556 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2754 4195 1353 Size: 1401 1441 3423 Fragments arranged by size: 3423 1441 1401 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2077 2095 2312 2420 2441 2498 Size: 393 391 54 18 217 108 21 57 Cuts at: 2498 2516 2543 2732 2759 2821 2921 3158 3275 Size: 18 27 189 27 62 100 237 117 Cuts at: 3275 3357 3444 3475 3487 3542 3566 4328 4925 Size: 82 87 31 12 55 24 762 597 Cuts at: 4925 5712 5980 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 762 597 393 391 268 237 217 189 117 108 100 87 82 62 57 55 54 31 27 27 24 21 18 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2660 2660 Size: 6265 EcoO109I rG'GnC_Cy Cuts at: 2416 2473 3399 3457 2416 Size: 57 926 58 5224 Fragments arranged by size: 5224 926 58 57 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2046 3327 3498 5412 1678 Size: 368 1281 171 1914 2531 Fragments arranged by size: 2531 1914 1281 368 171 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 3461 3493 4906 Size: 32 135 32 1010 32 847 32 1413 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1413 1010 847 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 3212 748 Size: 2464 3801 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 3456 5379 5563 1305 Size: 1374 777 1923 184 2007 Fragments arranged by size: 2007 1923 1374 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3158 10 Size: 54 1425 343 1326 3117 Fragments arranged by size: 3117 1425 1326 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2625 2751 2798 4223 4582 4741 518 Size: 648 1459 126 47 1425 359 159 2042 Fragments arranged by size: 2042 1459 1425 648 359 159 126 47 MspA1I CmG'CkG Cuts at: 1606 2521 3507 3681 4358 5506 5751 1606 Size: 915 986 174 677 1148 245 2120 Fragments arranged by size: 2120 1148 986 915 677 245 174 NcoI C'CATG_G Cuts at: 513 1065 2811 513 Size: 552 1746 3967 Fragments arranged by size: 3967 1746 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3623 5168 1132 Size: 97 2394 1545 2229 Fragments arranged by size: 2394 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3395 1348 Size: 2047 4218 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PfoI T'CCnGG_A Cuts at: 2397 2418 2397 Size: 21 6244 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2416 3399 3457 2416 Size: 983 58 5224 Fragments arranged by size: 5224 983 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 6265 PstI C_TGCA'G Cuts at: 830 2445 3024 3066 3617 830 Size: 1615 579 42 551 3478 Fragments arranged by size: 3478 1615 579 551 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2521 3507 2521 Size: 986 5279 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2754 2754 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3066 3617 3066 Size: 551 5714 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2441 3020 3062 3613 4748 5429 5620 Size: 219 1396 579 42 551 1135 681 191 Cuts at: 5620 826 Size: 1471 Fragments arranged by size: 1471 1396 1135 681 579 551 219 191 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3592 1872 Size: 1720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2068 2636 2657 3041 3107 4369 Size: 197 926 125 568 21 384 66 1262 Cuts at: 4369 5270 5532 820 Size: 901 262 1553 Fragments arranged by size: 1553 1262 926 901 568 384 262 197 125 66 21 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 2495 2570 2909 1517 Size: 978 75 339 4873 Fragments arranged by size: 4873 978 339 75 StyI C'CwwG_G Cuts at: 513 1065 2811 3123 3704 513 Size: 552 1746 312 581 3074 Fragments arranged by size: 3074 1746 581 552 312 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4292 4309 4462 4647 5092 325 Size: 1255 2712 17 153 185 445 1498 Fragments arranged by size: 2712 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4511 6223 96 Size: 274 80 33 51 1443 2534 1712 138 Fragments arranged by size: 2534 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 3352 3384 6225 6257 2414 Size: 32 906 32 2841 32 2422 Fragments arranged by size: 2841 2422 906 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3166 3439 3166 Size: 273 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3590 1870 Size: 1720 4545 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI