Restriction map for
pFN21AB7246
(Linear) (Six-base) MAPSORT of: KIBB7246.seq Check: 2578 from: 1 to: 1560
KIBB7246.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 72 90 307 816 916 1537 1560
Size: 18 54 18 217 509 100 621 23
Fragments arranged by size:
621 509 217 100 54 23 18 18
AfeI AGC'GCT
Cuts at: 0 39 1320 1491 1560
Size: 39 1281 171 69
Fragments arranged by size:
1281 171 69 39
AflIII A'CryG_T
Cuts at: 0 788 1560
Size: 788 772
AleI CACnn'nnGTG
Cuts at: 0 620 793 1560
Size: 620 173 767
Fragments arranged by size:
767 620 173
AlwNI CAG_nnn'CTG
Cuts at: 0 273 471 1014 1164 1194 1560
Size: 273 198 543 150 30 366
Fragments arranged by size:
543 366 273 198 150 30
ApaI G_GGCC'C
Cuts at: 0 24 1560
Size: 24 1536
ApaLI G'TGCA_C
Cuts at: 0 1281 1560
Size: 1281 279
BanI G'GyrC_C
Cuts at: 0 137 1560
Size: 137 1423
BanII G_rGCy'C
Cuts at: 0 24 224 546 1444 1560
Size: 24 200 322 898 116
Fragments arranged by size:
898 322 200 116 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1528 1549 1560
Size: 1528 21 11
Fragments arranged by size:
1528 21 11
BbvCI CC'TCA_GC
Cuts at: 0 893 899 1560
Size: 893 6 661
Fragments arranged by size:
893 661 6
BglI GCCn_nnn'nGGC
Cuts at: 0 146 467 1560
Size: 146 321 1093
Fragments arranged by size:
1093 321 146
BglII A'GATC_T
Cuts at: 0 983 1560
Size: 983 577
BlpI GC'TnA_GC
Cuts at: 0 45 1079 1560
Size: 45 1034 481
Fragments arranged by size:
1034 481 45
Bme1580I G_kGCm'C
Cuts at: 0 24 235 1285 1560
Size: 24 211 1050 275
Fragments arranged by size:
1050 275 211 24
BmgBI CAC'GTC
Cuts at: 0 791 1560
Size: 791 769
BmrI ACTGGGnnnn_n'
Cuts at: 0 300 1560
Size: 300 1260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 1560
Size: 742 818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 436 493 511 538 727 754 1153
Size: 415 21 57 18 27 189 27 399
Cuts at: 1153 1270 1352 1439 1470 1482 1560
Size: 117 82 87 31 12 78
Fragments arranged by size:
415 399 189 117 87 82 78 57
31 27 27 21 18 12
Bpu10I CC'TnA_GC
Cuts at: 0 505 679 893 899 1112 1560
Size: 505 174 214 6 213 448
Fragments arranged by size:
505 448 214 213 174 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 637 652 1021 1087 1560
Size: 48 589 15 369 66 473
Fragments arranged by size:
589 473 369 66 48 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 1560
Size: 325 1235
BsaBI GATnn'nnATC
Cuts at: 0 295 1560
Size: 295 1265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 571 577 601 607 1347 1377
Size: 409 30 132 6 24 6 740 30
Cuts at: 1377 1560
Size: 183
Fragments arranged by size:
740 409 183 132 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 598 619 622 883 1273 1293 1349
Size: 595 3 21 3 261 390 20 56
Cuts at: 1349 1560
Size: 211
Fragments arranged by size:
595 390 261 211 56 21 20 3
3
BseYI C'CCAG_C
Cuts at: 0 183 759 944 1560
Size: 183 576 185 616
Fragments arranged by size:
616 576 185 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1284 1386 1560
Size: 1284 102 174
Fragments arranged by size:
1284 174 102
BsiHKAI G_wGCw'C
Cuts at: 0 1204 1285 1360 1560
Size: 1204 81 75 200
Fragments arranged by size:
1204 200 81 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 1560
Size: 412 1148
Bsp1286I G_dGCh'C
Cuts at: 0 24 224 235 546 1204 1285 1360 1444
Size: 24 200 11 311 658 81 75 84
Cuts at: 1444 1560
Size: 116
Fragments arranged by size:
658 311 200 116 84 81 75 24
11
BssSI C'ACGA_G
Cuts at: 0 152 1560
Size: 152 1408
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 1560
Size: 435 1125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 952 1560
Size: 952 608
BstYI r'GATC_y
Cuts at: 0 983 1560
Size: 983 577
BtgI C'CryG_G
Cuts at: 0 806 1560
Size: 806 754
ClaI AT'CG_AT
Cuts at: 0 299 1560
Size: 299 1261
DrdI GACnn_nn'nnGTC
Cuts at: 0 646 1530 1560
Size: 646 884 30
Fragments arranged by size:
884 646 30
EaeI y'GGCC_r
Cuts at: 0 1151 1560
Size: 1151 409
EarI CTCTTCn'nnn_
Cuts at: 0 749 1560
Size: 749 811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 72 90 307 415 436 493 511
Size: 18 54 18 217 108 21 57 18
Cuts at: 511 538 727 754 816 916 1153 1270 1352
Size: 27 189 27 62 100 237 117 82
Cuts at: 1352 1439 1470 1482 1537 1560
Size: 87 31 12 55 23
Fragments arranged by size:
237 217 189 117 108 100 87 82
62 57 55 54 31 27 27 23
21 18 18 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 655 1560
Size: 655 905
EcoO109I rG'GnC_Cy
Cuts at: 0 411 468 1394 1452 1560
Size: 411 57 926 58 108
Fragments arranged by size:
926 411 108 58 57
HaeII r_GCGC'y
Cuts at: 0 41 1322 1493 1560
Size: 41 1281 171 67
Fragments arranged by size:
1281 171 67 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 1456 1488 1560
Size: 577 32 847 32 72
Fragments arranged by size:
847 577 72 32 32
HindIII A'AGCT_T
Cuts at: 0 1207 1560
Size: 1207 353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1451 1560
Size: 674 777 109
Fragments arranged by size:
777 674 109
MscI TGG'CCA
Cuts at: 0 1153 1560
Size: 1153 407
MslI CAynn'nnrTG
Cuts at: 0 620 746 793 1560
Size: 620 126 47 767
Fragments arranged by size:
767 620 126 47
MspA1I CmG'CkG
Cuts at: 0 516 1502 1560
Size: 516 986 58
Fragments arranged by size:
986 516 58
NcoI C'CATG_G
Cuts at: 0 806 1560
Size: 806 754
PasI CC'CwG_GG
Cuts at: 0 1390 1560
Size: 1390 170
PfoI T'CCnGG_A
Cuts at: 0 392 413 1560
Size: 392 21 1147
Fragments arranged by size:
1147 392 21
PpuMI rG'GwC_Cy
Cuts at: 0 411 1394 1452 1560
Size: 411 983 58 108
Fragments arranged by size:
983 411 108 58
PspOMI G'GGCC_C
Cuts at: 0 20 1560
Size: 20 1540
PstI C_TGCA'G
Cuts at: 0 440 1019 1061 1560
Size: 440 579 42 499
Fragments arranged by size:
579 499 440 42
PvuII CAG'CTG
Cuts at: 0 516 1502 1560
Size: 516 986 58
Fragments arranged by size:
986 516 58
SapI GCTCTTCn'nnn_
Cuts at: 0 749 1560
Size: 749 811
SbfI CC_TGCA'GG
Cuts at: 0 1061 1560
Size: 1061 499
SfcI C'TryA_G
Cuts at: 0 436 1015 1057 1560
Size: 436 579 42 503
Fragments arranged by size:
579 503 436 42
SmlI C'TyrA_G
Cuts at: 0 63 631 652 1036 1102 1560
Size: 63 568 21 384 66 458
Fragments arranged by size:
568 458 384 66 63 21
StuI AGG'CCT
Cuts at: 0 490 565 904 1560
Size: 490 75 339 656
Fragments arranged by size:
656 490 339 75
StyI C'CwwG_G
Cuts at: 0 806 1118 1560
Size: 806 312 442
Fragments arranged by size:
806 442 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 1347 1379 1560
Size: 409 32 906 32 181
Fragments arranged by size:
906 409 181 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1161 1434 1560
Size: 1161 273 126
Fragments arranged by size:
1161 273 126
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApaI ApaLI BanI
BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BssSI BstAPI
BstXI BstYI BtgI ClaI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NcoI PasI PfoI PpuMI PspOMI PstI PvuII
SapI SbfI SfcI SmlI StuI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BmtI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI XmnI ZraI