Restriction map for
pFN21AB7243
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmI BspDI BspHI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI Tru9I
Tsp509I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 585 TCCGGA T^CCGGA
AcyI 1285 GGCGTC G[GA]^CG[CT]C
AflIII 158 ACACGT A^C[GA][CT]GT
AocI 1100 CCTCAGG CC^T[ACGT]AGG
AspI 1012 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1295 TGGCCA TGG^CCA
BamHI 575 GGATCC G^GATCC
BbsI 25 GTCTTC GAAGAC(2/6)
BbvCI 822 CCTCAGC CCTCAGC(-5/-2)
BcgI 761 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaHI 1285 GGCGTC G[GA]^CG[CT]C
BsaWI 585 TCCGGA [AT]^CCGG[AT]
BseAI 585 TCCGGA T^CCGGA
BsiEI 1247 CGGCCG CG[GA][CT]^CG
BsmBI 948 CGTCTC CGTCTC(1/5)
Bsp1407I 1109 TGTACA T^GTACA
BspEI 585 TCCGGA T^CCGGA
BspMI 21 GCAGGT ACCTGC(4/8)
BsrGI 1109 TGTACA T^GTACA
BstXI 356 CCACGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 575 GGATCC [GA]^GATC[CT]
Bsu36I 1100 CCTCAGG CC^T[ACGT]AGG
DraIII 869 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 706 GACTACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1247 CGGCCG C^GGCCG
EclXI 1247 CGGCCG C^GGCCG
Eco52I 1247 CGGCCG C^GGCCG
Eco81I 1100 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1346 CCTTGG C^C[AT][AT]GG
HaeII 315 GGCGCT [GA]GCGC^[CT]
Hin1I 1285 GGCGTC G[GA]^CG[CT]C
HincII 526 GTCAAC GT[CT]^[GA]AC
HindII 526 GTCAAC GT[CT]^[GA]AC
MflI 575 GGATCC [GA]^GATC[CT]
MluNI 1295 TGGCCA TGG^CCA
MroI 585 TCCGGA T^CCGGA
MscI 1295 TGGCCA TGG^CCA
NaeI 1257 GCCGGC GCC^GGC
NgoMIV 1257 GCCGGC G^CCGGC
NspI 1220 ACATGC [GA]CATG^[CT]
PaeR7I 1168 CTCGAG C^TCGAG
PflFI 1012 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 865 GAGTC GAGTC(4/5)
PpuMI 443 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 543 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 295 CTGCAG CTGCA^G
PvuII 981 CAGCTG CAG^CTG
SacI 481 GAGCTC GAGCT^C
SapI 1356 GCTCTTC GCTCTTC(1/4)
SbfI 294 CCTGCAGG CCTGCA^GG
ScaI 554 AGTACT AGT^ACT
SfiI 489 GGCCGGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1168 CTCGAG C^T[CT][GA]AG
Sse8387I 294 CCTGCAGG CCTGCA^GG
SspBI 1109 TGTACA T^GTACA
StyI 1346 CCTTGG C^C[AT][AT]GG
TaqI 1169 TCGA T^CGA
TfiI 251 GAATC G^A[AT]TC
Tth111I 1012 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1169 TCGA T^CGA
XcmI 839 CCACACTGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1168 CTCGAG C^TCGAG
XhoII 575 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 715 GTCTAC GT^[AC][GT]AC
1018 GTCTAC
ApaI 654 GGGCCC GGGCC^C
852 GGGCCC
AvaI 48 CTCGGG C^[CT]CG[GA]G
1168 CTCGAG
BsgI 275 CTGCAC GTGCAG(16/14)
1126 CTGCAC
BsoBI 48 CTCGGG C^[CT]CG[GA]G
1168 CTCGAG
BsrFI 452 GCCGGT [GA]^CCGG[CT]
1257 GCCGGC
BssSI 1129 CACGAG CACGAG(-5/-1)
769 CTCGTG
Cfr10I 452 GCCGGT [GA]^CCGG[CT]
1257 GCCGGC
DsaI 371 CCACGG C^C[GA][CT]GG
461 CCGTGG
EarI 166 CTCTTC CTCTTC(1/4)
1357 CTCTTC
EcoNI 84 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1125 CCTGCACGAGG
HinfI 251 GAATC G^A[ACGT]TC
865 GAGTC
Ksp632I 166 CTCTTC CTCTTC(1/4)
1357 CTCTTC
PflMI 219 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
441 CCAGGTCCTGG
SfcI 295 CTGCAG C^T[GA][CT]AG
708 CTACAG
StuI 60 AGGCCT AGG^CCT
1104 AGGCCT
Van91I 219 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
441 CCAGGTCCTGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI