(Circular) (Six-base) MAPSORT of: pFN21AB7243.seq Check: 3601 from: 1 to: 6079 pFN21AB7243 6079 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2721 3024 3422 1937 Size: 784 303 398 4594 Fragments arranged by size: 4594 784 398 303 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4142 5526 5794 1632 Size: 2510 1384 268 1917 Fragments arranged by size: 2510 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2163 4978 5955 1203 Size: 960 2815 977 1327 Fragments arranged by size: 2815 1327 977 960 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 2105 2213 2648 1166 Size: 939 108 435 4597 Fragments arranged by size: 4597 939 435 108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2408 1184 Size: 1224 4855 AlwNI CAG_nnn'CTG Cuts at: 2188 2230 2452 2761 3822 5394 2188 Size: 42 222 309 1061 1572 2873 Fragments arranged by size: 2873 1572 1061 309 222 42 ApaI G_GGCC'C Cuts at: 2663 2861 2663 Size: 198 5881 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2053 3173 3404 1870 Size: 73 110 1120 231 4545 Fragments arranged by size: 4545 1120 231 110 73 BamHI G'GATC_C Cuts at: 2580 3409 3776 2580 Size: 829 367 4883 Fragments arranged by size: 4883 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2480 2510 2609 3400 4855 Size: 325 217 627 693 30 99 791 1455 Cuts at: 4855 618 Size: 1842 Fragments arranged by size: 1842 1455 791 693 627 325 217 99 30 BanII G_rGCy'C Cuts at: 1391 2156 2490 2663 2861 3398 1391 Size: 765 334 173 198 537 4072 Fragments arranged by size: 4072 765 537 334 198 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 928 Size: 971 124 4984 Fragments arranged by size: 4984 971 124 BbvCI CC'TCA_GC Cuts at: 2828 2828 Size: 6079 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2755 2789 4270 4304 1903 Size: 34 818 34 1481 34 3678 Fragments arranged by size: 3678 1481 818 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2304 2501 2703 Size: 107 122 71 631 605 632 197 202 Cuts at: 2703 3332 4690 136 Size: 629 1358 1525 Fragments arranged by size: 1525 1358 632 631 629 605 202 197 122 107 71 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 2396 2762 2900 3496 2396 Size: 366 138 596 4979 Fragments arranged by size: 4979 596 366 138 Bme1580I G_kGCm'C Cuts at: 1790 2614 2663 2861 4140 5296 1790 Size: 824 49 198 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 824 198 49 BmgBI CAC'GTC Cuts at: 1318 2166 1318 Size: 848 5231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3227 3810 4768 Size: 416 53 83 399 24 1810 583 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1810 1753 958 583 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 2964 2968 4739 1239 Size: 1353 372 4 1771 2579 Fragments arranged by size: 2579 1771 1353 372 4 Bpu10I CC'TnA_GC Cuts at: 2828 2828 Size: 6079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2065 2466 3152 4742 882 Size: 617 566 401 686 1590 2219 Fragments arranged by size: 2219 1590 686 617 566 401 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3291 3886 4268 4903 Size: 53 83 186 936 1758 595 382 635 Cuts at: 4903 275 Size: 1451 Fragments arranged by size: 1758 1451 936 635 595 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2590 4512 4910 5184 5331 5742 2590 Size: 1922 398 274 147 411 2927 Fragments arranged by size: 2927 1922 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 2937 2967 1178 Size: 30 165 30 664 30 840 30 4290 Fragments arranged by size: 4290 840 664 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2146 2251 2330 2609 2762 3170 1186 Size: 889 71 105 79 279 153 408 4095 Fragments arranged by size: 4095 889 408 279 153 105 79 71 BseYI C'CCAG_C Cuts at: 2044 2343 3167 5282 5778 2044 Size: 299 824 2115 496 2345 Fragments arranged by size: 2345 2115 824 496 299 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2265 3116 1163 Size: 573 529 851 4126 Fragments arranged by size: 4126 851 573 529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3255 3529 4290 5318 1540 Size: 275 186 1254 274 761 1028 2301 Fragments arranged by size: 2301 1254 1028 761 275 274 186 BsiHKAI G_wGCw'C Cuts at: 2112 2490 2681 4140 4225 5296 2112 Size: 378 191 1459 85 1071 2895 Fragments arranged by size: 2895 1459 1071 378 191 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 BsmBI CGTCTCn'nnnn_ Cuts at: 2959 2959 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2156 2490 2614 2663 2681 2861 Size: 399 322 44 334 124 49 18 180 Cuts at: 2861 3398 4140 4225 5296 1391 Size: 537 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 334 322 180 124 85 49 44 18 BspEI T'CCGG_A Cuts at: 2590 5742 2590 Size: 3152 2927 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 3434 844 Size: 1173 1417 3489 Fragments arranged by size: 3489 1417 1173 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2457 3262 4723 4910 1700 Size: 110 647 805 1461 187 2869 Fragments arranged by size: 2869 1461 805 647 187 110 BsrGI T'GTAC_A Cuts at: 96 3114 96 Size: 3018 3061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 2774 3134 4139 5151 2774 Size: 360 1005 1012 3702 Fragments arranged by size: 3702 1012 1005 360 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstXI CCAn_nnnn'nTGG Cuts at: 2368 2368 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2580 3409 3776 4161 4178 5630 5716 5728 Size: 608 829 367 385 17 1452 86 12 Cuts at: 5728 6074 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 829 608 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3106 3106 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 2376 2466 513 Size: 552 939 372 90 4126 Fragments arranged by size: 4126 939 552 372 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2879 2879 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2717 5086 809 Size: 507 1401 2369 1802 Fragments arranged by size: 2369 1802 1401 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 3252 3300 Size: 54 1425 50 275 18 663 759 48 Cuts at: 3300 3526 4415 6044 8 Size: 226 889 1629 43 Fragments arranged by size: 1629 1425 889 759 663 275 226 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3252 3526 1537 Size: 275 1440 274 4090 Fragments arranged by size: 4090 1440 275 274 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 3368 4009 1353 Size: 824 1191 641 3423 Fragments arranged by size: 3423 1191 824 641 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4666 5040 5186 387 Size: 1064 138 3077 374 146 1280 Fragments arranged by size: 3077 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2488 2488 Size: 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2592 2964 2968 4142 4739 5526 5794 Size: 393 960 372 4 1174 597 787 268 Cuts at: 5794 1239 Size: 1524 Fragments arranged by size: 1524 1174 960 787 597 393 372 268 4 EcoNI CCTnn'n_nnAGG Cuts at: 2093 3134 2093 Size: 1041 5038 EcoO109I rG'GnC_Cy Cuts at: 2049 2227 2249 2449 2628 2659 2660 2049 Size: 178 22 200 179 31 1 5468 Fragments arranged by size: 5468 200 179 178 31 22 1 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6079 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2324 5226 1678 Size: 646 2902 2531 Fragments arranged by size: 2902 2531 646 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2565 2572 2597 Size: 32 135 32 566 32 395 7 25 Cuts at: 2597 2604 2937 2969 4720 4752 4794 4826 1373 Size: 7 333 32 1751 32 42 32 2626 Fragments arranged by size: 2626 1751 566 395 333 135 42 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2533 3423 3669 669 Size: 988 281 595 890 246 3079 Fragments arranged by size: 3079 988 890 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2501 2733 5193 5377 1305 Size: 1196 232 2460 184 2007 Fragments arranged by size: 2460 2007 1196 232 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3302 10 Size: 54 1425 343 1470 2787 Fragments arranged by size: 2787 1470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2105 2213 2648 4037 4396 4555 518 Size: 648 939 108 435 1389 359 159 2042 Fragments arranged by size: 2042 1389 939 648 435 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2832 2863 2899 2988 3037 3084 3495 4172 Size: 1226 31 36 89 49 47 411 677 Cuts at: 4172 5320 5565 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1226 1148 677 411 245 89 49 47 36 31 NaeI GCC'GGC Cuts at: 3264 3264 Size: 6079 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NgoMIV G'CCGG_C Cuts at: 3262 3262 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 3526 3526 Size: 6079 NspI r_CATG'y Cuts at: 1132 1229 3229 3437 4982 1132 Size: 97 2000 208 1545 2229 Fragments arranged by size: 2229 2000 1545 208 97 PasI CC'CwG_GG Cuts at: 1348 2854 3071 1348 Size: 1506 217 4356 Fragments arranged by size: 4356 1506 217 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2230 2452 1108 Size: 1122 222 4735 Fragments arranged by size: 4735 1122 222 PfoI T'CCnGG_A Cuts at: 2015 2964 2015 Size: 949 5130 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2449 Size: 6079 PshAI GACnn'nnGTC Cuts at: 1613 2552 1613 Size: 939 5140 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2659 2857 2659 Size: 198 5881 PspXI vC'TCGA_Gb Cuts at: 3173 3173 Size: 6079 PstI C_TGCA'G Cuts at: 830 2304 3431 830 Size: 1474 1127 3478 Fragments arranged by size: 3478 1474 1127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 2988 2988 Size: 6079 SacI G_AGCT'C Cuts at: 2490 2490 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 3368 3368 Size: 6079 SbfI CC_TGCA'GG Cuts at: 2304 3431 2304 Size: 1127 4952 ScaI AGT'ACT Cuts at: 2561 4327 6039 2561 Size: 1766 1712 2601 Fragments arranged by size: 2601 1766 1712 SfcI C'TryA_G Cuts at: 826 1045 2300 2713 3427 4562 5243 5434 826 Size: 219 1255 413 714 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 714 681 413 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2501 2501 Size: 6079 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3173 4183 5084 5346 820 Size: 197 926 1230 1010 901 262 1553 Fragments arranged by size: 1553 1230 1010 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2067 3111 1517 Size: 550 1044 4485 Fragments arranged by size: 4485 1044 550 StyI C'CwwG_G Cuts at: 513 1065 2004 3351 3518 513 Size: 552 939 1347 167 3074 Fragments arranged by size: 3074 1347 939 552 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 2459 2597 2651 2696 3286 4106 Size: 1255 794 85 138 54 45 590 820 Cuts at: 4106 4123 4276 4461 4906 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 820 794 590 445 185 153 138 85 54 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2559 3114 4325 Size: 274 80 33 51 1443 582 555 1211 Cuts at: 4325 6037 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1211 582 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 2349 2381 2865 2897 6039 6071 2067 Size: 32 250 32 484 32 3142 32 2075 Fragments arranged by size: 3142 2075 484 250 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 1316 Size: 1704 4375 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 2851 Size: 6079 XhoI C'TCGA_G Cuts at: 1943 3173 1943 Size: 1230 4849 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI