Restriction map for
pFN21AB7243
(Linear) (Six-base) MAPSORT of: KIBB7243.seq Check: 488 from: 1 to: 1374
KIBB7243.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 716 1019 1374
Size: 716 303 355
Fragments arranged by size:
716 355 303
AflIII A'CryG_T
Cuts at: 0 158 1374
Size: 158 1216
AleI CACnn'nnGTG
Cuts at: 0 100 208 643 1374
Size: 100 108 435 731
Fragments arranged by size:
731 435 108 100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 1374
Size: 403 971
AlwNI CAG_nnn'CTG
Cuts at: 0 183 225 447 756 1374
Size: 183 42 222 309 618
Fragments arranged by size:
618 309 222 183 42
ApaI G_GGCC'C
Cuts at: 0 658 856 1374
Size: 658 198 518
Fragments arranged by size:
658 518 198
AvaI C'yCGr_G
Cuts at: 0 48 1168 1374
Size: 48 1120 206
Fragments arranged by size:
1120 206 48
BamHI G'GATC_C
Cuts at: 0 575 1374
Size: 575 799
BanI G'GyrC_C
Cuts at: 0 475 505 604 1374
Size: 475 30 99 770
Fragments arranged by size:
770 475 99 30
BanII G_rGCy'C
Cuts at: 0 151 485 658 856 1374
Size: 151 334 173 198 518
Fragments arranged by size:
518 334 198 173 151
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 1374
Size: 18 1356
BbvCI CC'TCA_GC
Cuts at: 0 823 1374
Size: 823 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 750 784 1374
Size: 750 34 590
Fragments arranged by size:
750 590 34
BglI GCCn_nnn'nGGC
Cuts at: 0 299 496 698 1327 1374
Size: 299 197 202 629 47
Fragments arranged by size:
629 299 202 197 47
BlpI GC'TnA_GC
Cuts at: 0 391 757 895 1374
Size: 391 366 138 479
Fragments arranged by size:
479 391 366 138
Bme1580I G_kGCm'C
Cuts at: 0 609 658 856 1374
Size: 609 49 198 518
Fragments arranged by size:
609 518 198 49
BmgBI CAC'GTC
Cuts at: 0 161 1374
Size: 161 1213
BmrI ACTGGGnnnn_n'
Cuts at: 0 1222 1374
Size: 1222 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 959 963 1374
Size: 587 372 4 411
Fragments arranged by size:
587 411 372 4
Bpu10I CC'TnA_GC
Cuts at: 0 823 1374
Size: 823 551
BsaI GGTCTCn'nnnn_
Cuts at: 0 60 461 1147 1374
Size: 60 401 686 227
Fragments arranged by size:
686 401 227 60
BsaHI Gr'CG_yC
Cuts at: 0 1286 1374
Size: 1286 88
BsaWI w'CCGG_w
Cuts at: 0 585 1374
Size: 585 789
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 932 962 1374
Size: 62 30 840 30 412
Fragments arranged by size:
840 412 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 141 246 325 604 757 1165 1374
Size: 70 71 105 79 279 153 408 209
Fragments arranged by size:
408 279 209 153 105 79 71 70
BseYI C'CCAG_C
Cuts at: 0 39 338 1162 1374
Size: 39 299 824 212
Fragments arranged by size:
824 299 212 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1111 1374
Size: 260 851 263
Fragments arranged by size:
851 263 260
BsiEI CG_ry'CG
Cuts at: 0 1250 1374
Size: 1250 124
BsiHKAI G_wGCw'C
Cuts at: 0 107 485 676 1374
Size: 107 378 191 698
Fragments arranged by size:
698 378 191 107
BsmBI CGTCTCn'nnnn_
Cuts at: 0 954 1374
Size: 954 420
Bsp1286I G_dGCh'C
Cuts at: 0 107 151 485 609 658 676 856 1374
Size: 107 44 334 124 49 18 180 518
Fragments arranged by size:
518 334 180 124 107 49 44 18
BspEI T'CCGG_A
Cuts at: 0 585 1374
Size: 585 789
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 1374
Size: 12 1362
BsrFI r'CCGG_y
Cuts at: 0 452 1257 1374
Size: 452 805 117
Fragments arranged by size:
805 452 117
BsrGI T'GTAC_A
Cuts at: 0 1109 1374
Size: 1109 265
BssSI C'ACGA_G
Cuts at: 0 769 1129 1374
Size: 769 360 245
Fragments arranged by size:
769 360 245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 363 1374
Size: 363 1011
BstYI r'GATC_y
Cuts at: 0 575 1374
Size: 575 799
Bsu36I CC'TnA_GG
Cuts at: 0 1101 1374
Size: 1101 273
BtgI C'CryG_G
Cuts at: 0 371 461 1374
Size: 371 90 913
Fragments arranged by size:
913 371 90
DraIII CAC_nnn'GTG
Cuts at: 0 874 1374
Size: 874 500
DrdI GACnn_nn'nnGTC
Cuts at: 0 712 1374
Size: 712 662
EaeI y'GGCC_r
Cuts at: 0 488 1247 1295 1374
Size: 488 759 48 79
Fragments arranged by size:
759 488 79 48
EagI C'GGCC_G
Cuts at: 0 1247 1374
Size: 1247 127
EarI CTCTTCn'nnn_
Cuts at: 0 172 1363 1374
Size: 172 1191 11
Fragments arranged by size:
1191 172 11
EcoICRI GAG'CTC
Cuts at: 0 483 1374
Size: 483 891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 959 963 1374
Size: 587 372 4 411
Fragments arranged by size:
587 411 372 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 1129 1374
Size: 88 1041 245
Fragments arranged by size:
1041 245 88
EcoO109I rG'GnC_Cy
Cuts at: 0 44 222 244 444 623 654 655 1374
Size: 44 178 22 200 179 31 1 719
Fragments arranged by size:
719 200 179 178 44 31 22 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 576 1374
Size: 576 798
HaeII r_GCGC'y
Cuts at: 0 319 1374
Size: 319 1055
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 560 567 592 599 932 964
Size: 133 32 395 7 25 7 333 32
Cuts at: 964 1374
Size: 410
Fragments arranged by size:
410 395 333 133 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 528 1374
Size: 528 846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 728 1374
Size: 496 232 646
Fragments arranged by size:
646 496 232
MscI TGG'CCA
Cuts at: 0 1297 1374
Size: 1297 77
MslI CAynn'nnrTG
Cuts at: 0 100 208 643 1374
Size: 100 108 435 731
Fragments arranged by size:
731 435 108 100
MspA1I CmG'CkG
Cuts at: 0 827 858 894 983 1032 1079 1374
Size: 827 31 36 89 49 47 295
Fragments arranged by size:
827 295 89 49 47 36 31
NaeI GCC'GGC
Cuts at: 0 1259 1374
Size: 1259 115
NgoMIV G'CCGG_C
Cuts at: 0 1257 1374
Size: 1257 117
NspI r_CATG'y
Cuts at: 0 1224 1374
Size: 1224 150
PasI CC'CwG_GG
Cuts at: 0 849 1066 1374
Size: 849 217 308
Fragments arranged by size:
849 308 217
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 447 1374
Size: 225 222 927
Fragments arranged by size:
927 225 222
PfoI T'CCnGG_A
Cuts at: 0 10 959 1374
Size: 10 949 415
Fragments arranged by size:
949 415 10
PpuMI rG'GwC_Cy
Cuts at: 0 444 1374
Size: 444 930
PshAI GACnn'nnGTC
Cuts at: 0 547 1374
Size: 547 827
PspOMI G'GGCC_C
Cuts at: 0 654 852 1374
Size: 654 198 522
Fragments arranged by size:
654 522 198
PspXI vC'TCGA_Gb
Cuts at: 0 1168 1374
Size: 1168 206
PstI C_TGCA'G
Cuts at: 0 299 1374
Size: 299 1075
PvuII CAG'CTG
Cuts at: 0 983 1374
Size: 983 391
SacI G_AGCT'C
Cuts at: 0 485 1374
Size: 485 889
SapI GCTCTTCn'nnn_
Cuts at: 0 1363 1374
Size: 1363 11
SbfI CC_TGCA'GG
Cuts at: 0 299 1374
Size: 299 1075
ScaI AGT'ACT
Cuts at: 0 556 1374
Size: 556 818
SfcI C'TryA_G
Cuts at: 0 295 708 1374
Size: 295 413 666
Fragments arranged by size:
666 413 295
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 496 1374
Size: 496 878
SmlI C'TyrA_G
Cuts at: 0 1168 1374
Size: 1168 206
StuI AGG'CCT
Cuts at: 0 62 1106 1374
Size: 62 1044 268
Fragments arranged by size:
1044 268 62
StyI C'CwwG_G
Cuts at: 0 1346 1374
Size: 1346 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 454 592 646 691 1281 1374
Size: 369 85 138 54 45 590 93
Fragments arranged by size:
590 369 138 93 85 54 45
TatI w'GTAC_w
Cuts at: 0 554 1109 1374
Size: 554 555 265
Fragments arranged by size:
555 554 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 344 376 860 892 1374
Size: 62 32 250 32 484 32 482
Fragments arranged by size:
484 482 250 62 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 1374
Size: 1015 359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1374
Size: 846 528
XhoI C'TCGA_G
Cuts at: 0 1168 1374
Size: 1168 206
Enzymes that do cut:
AccI AflIII AleI AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SapI SbfI
ScaI SfcI SfiI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BclI BfrBI BglII BmtI BplI
BpuEI BsaAI BsaBI BsiWI BsmI BspHI BsrBI BsrDI
BssHII BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI EciI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PsrI PvuI RsrII SacII SalI
SanDI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI XmnI ZraI