Restriction map for
pFN21AB7241
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# NO CUT ENZYMES ( 178 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MluI MluNI MroI
MscI MslI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI TfiI TspRI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 292 GACGTC GACGT^C
AciI 284 GCGG CCGC(-3/-1)
AcyI 292 GACGTC G[GA]^CG[CT]C
AlwI 220 GGATC GGATC(4/5)
BanI 209 GGTGCC G^G[CT][GA]CC
BanII 427 GGGCTC G[GA]GC[CT]^C
BmyI 427 GGGCTC G[AGT]GC[ACT]^C
BsaHI 292 GACGTC G[GA]^CG[CT]C
BsaI 193 GAGACC GGTCTC(1/5)
BsaJI 197 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 357 TCCGGT [AT]^CCGG[AT]
BsmAI 193 GAGAC GTCTC(1/5)
BsmFI 32 GTCCC GGGAC(10/14)
Bsp1286I 427 GGGCTC G[AGT]GC[ACT]^C
BspHI 92 TCATGA T^CATGA
BspMI 167 ACCTGC ACCTGC(4/8)
BsrDI 407 GCAATG GCAATG(2/0)
BssKI 396 CCTGG ^CC[ACGT]GG
BstNI 396 CCTGG CC^[AT]GG
BstYI 220 GGATCT [GA]^GATC[CT]
CfoI 171 GCGC GCG^C
DpnII 221 GATC ^GATC
EcoRII 396 CCTGG ^CC[AT]GG
EcoT14I 197 CCAAGG C^C[AT][AT]GG
HaeIII 7 GGCC GG^CC
HapII 358 CCGG C^CGG
HhaI 171 GCGC GCG^C
Hin1I 292 GACGTC G[GA]^CG[CT]C
HinP1I 171 GCGC G^CGC
HinfI 24 GAGTC G^A[ACGT]TC
HpaII 358 CCGG C^CGG
MaeII 293 ACGT A^CGT
MaeIII 305 GTGAC ^GT[ACGT]AC
MboI 221 GATC ^GATC
MfeI 129 CAATTG C^AATTG
MflI 220 GGATCT [GA]^GATC[CT]
MseI 79 TTAA T^TAA
MspI 358 CCGG C^CGG
MunI 129 CAATTG C^AATTG
MvaI 396 CCTGG CC^[AT]GG
NdeII 221 GATC ^GATC
PflMI 21 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 24 GAGTC GAGTC(4/5)
PpuMI 30 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 11 CTGCAG CTGCA^G
RcaI 92 TCATGA T^CATGA
Sau3AI 221 GATC ^GATC
SbfI 10 CCTGCAGG CCTGCA^GG
ScrFI 396 CCTGG CC^[ACGT]GG
SfcI 11 CTGCAG C^T[GA][CT]AG
Sse8387I 10 CCTGCAGG CCTGCA^GG
StyI 197 CCAAGG C^C[AT][AT]GG
Tru9I 79 TTAA T^TAA
Tsp45I 305 GTGAC ^GT[GC]AC
Tsp509I 130 AATT ^AATT
Van91I 21 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 220 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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AvaII 31 GGTCC G^G[AT]CC
72 GGTCC
BstAPI 4 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
248 GCAGCAGCTGC
Cac8I 4 GCAGGC GC[ACGT]^[ACGT]GC
244 GCTGGC
DraII 6 AGGCCCT [GA]G^G[ACGT]CC[CT]
30 GGGTCCC
EcoO109I 6 AGGCCCT [GA]G^G[ACGT]CC[CT]
30 GGGTCCC
HphI 300 GGTGA GGTGA(8/7)
236 TCACC
NlaIII 93 CATG CATG^
297 CATG
PvuII 242 CAGCTG CAG^CTG
252 CAGCTG
SfaNI 182 GATGC GCATC(5/9)
257 GCATC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI AlwNI BbvI BsiYI BslI
Cfr13I Fnu4HI ItaI MnlI MspA1I
MwoI NlaIV Sau96I TseI