(Linear) (Six-base) MAPSORT of: KIBB7241.seq  Check: 7027  from: 1  to: 441



KIBB7241.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AatII G_ACGT'C

Cuts at:      0     296     441 
   Size:        296     145
 
AlwNI CAG_nnn'CTG

Cuts at:      0      10      27     254     441 
   Size:         10      17     227     187

  Fragments arranged by size: 

                 227     187      17      10
 
BanI G'GyrC_C

Cuts at:      0     209     441 
   Size:        209     232
 
BanII G_rGCy'C

Cuts at:      0     431     441 
   Size:        431      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187     441 
   Size:        187     254
 
BsaHI Gr'CG_yC

Cuts at:      0     293     441 
   Size:        293     148
 
BsaWI w'CCGG_w

Cuts at:      0     357     441 
   Size:        357      84
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       9      39     193     223     441 
   Size:          9      30     154      30     218

  Fragments arranged by size: 

                 218     154      30      30       9
 
BseYI C'CCAG_C

Cuts at:      0     240     441 
   Size:        240     201
 
Bsp1286I G_dGCh'C

Cuts at:      0     431     441 
   Size:        431      10
 
BspHI T'CATG_A

Cuts at:      0      92     441 
   Size:         92     349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     176     441 
   Size:        176     265
 
BsrDI GCAATG_nn'

Cuts at:      0     414     441 
   Size:        414      27
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      10     254     441 
   Size:         10     244     187

  Fragments arranged by size: 

                 244     187      10
 
BstYI r'GATC_y

Cuts at:      0     220     441 
   Size:        220     221
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7      31     441 
   Size:          7      24     410

  Fragments arranged by size: 

                 410      24       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       9      15      41      47     212     244     366     398 
   Size:          9       6      26       6     165      32     122      32

Cuts at:    398     441 
   Size:         43

  Fragments arranged by size: 

                 165     122      43      32      32      26       9       6
                   6
 
MfeI C'AATT_G

Cuts at:      0     129     441 
   Size:        129     312
 
MspA1I CmG'CkG

Cuts at:      0     244     254     284     441 
   Size:        244      10      30     157

  Fragments arranged by size: 

                 244     157      30      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      27     441 
   Size:         27     414
 
PfoI T'CCnGG_A

Cuts at:      0     395     441 
   Size:        395      46
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     321     353     441 
   Size:        321      32      88

  Fragments arranged by size: 

                 321      88      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      31     441 
   Size:         31     410
 
PstI C_TGCA'G

Cuts at:      0      15     441 
   Size:         15     426
 
PvuII CAG'CTG

Cuts at:      0     244     254     441 
   Size:        244      10     187

  Fragments arranged by size: 

                 244     187      10
 
SanDI GG'GwC_CC

Cuts at:      0      31     441 
   Size:         31     410
 
SbfI CC_TGCA'GG

Cuts at:      0      15     441 
   Size:         15     426
 
SfcI C'TryA_G

Cuts at:      0      11     441 
   Size:         11     430
 
StyI C'CwwG_G

Cuts at:      0     197     441 
   Size:        197     244
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     140     172     193     225     441 
   Size:        140      32      21      32     216

  Fragments arranged by size: 

                 216     140      32      32      21
 
ZraI GAC'GTC

Cuts at:      0     294     441 
   Size:        294     147
 

 Enzymes that do cut:

    AatII    AlwNI     BanI    BanII     BsaI    BsaHI    BsaWI    BsaXI
    BseYI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI    BstYI EcoO109I
    Hin4I     MfeI   MspA1I    PflMI     PfoI     PpiI    PpuMI     PstI
    PvuII    SanDI     SbfI     SfcI     StyI     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI