Restriction map for
pFN21AB7236
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI ScaI
SfaNI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 917 CGCG CG^CG
AflIII 197 ACGTGT A^C[GA][CT]GT
Aor51HI 92 AGCGCT AGC^GCT
ApaI 289 GGGCCC GGGCC^C
BalI 107 TGGCCA TGG^CCA
BcgI 456 CGATGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 236 GTATCC GTATCC(6/5)
BclI 416 TGATCA T^GATCA
BlpI 303 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC
BsaI 631 GAGACC GGTCTC(1/5)
BsmBI 358 CGTCTC CGTCTC(1/5)
BspMI 594 GCAGGT ACCTGC(4/8)
BstBI 496 TTCGAA TT^CGAA
BstEII 564 GGTCACC G^GT[ACGT]ACC
BstF5I 774 CATCC GGATG(2/0)
BstPI 564 GGTCACC G^GT[ACGT]ACC
BstUI 917 CGCG CG^CG
Cac8I 434 GCCTGC GC[ACGT]^[ACGT]GC
CelII 303 GCTCAGC GC^T[ACGT]AGC
DraIII 406 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 92 AGCGCT AGC^GCT
EcoO65I 564 GGTCACC G^GT[ACGT]ACC
EcoT14I 879 CCTTGG C^C[AT][AT]GG
FauI 918 GCGGG CCCGC(4/6)
FbaI 416 TGATCA T^GATCA
FokI 774 CATCC GGATG(9/13)
HaeII 92 AGCGCT [GA]GCGC^[CT]
HincII 727 GTCAAC GT[CT]^[GA]AC
HindII 727 GTCAAC GT[CT]^[GA]AC
HindIII 583 AAGCTT A^AGCTT
MaeIII 565 GTCAC ^GT[ACGT]AC
MluNI 107 TGGCCA TGG^CCA
MscI 107 TGGCCA TGG^CCA
MslI 408 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 917 CGCG CG^CG
NspV 496 TTCGAA TT^CGAA
SbfI 435 CCTGCAGG CCTGCA^GG
SexAI 98 ACCAGGT A^CC[AT]GGT
SfuI 496 TTCGAA TT^CGAA
Sse8387I 435 CCTGCAGG CCTGCA^GG
StyI 879 CCTTGG C^C[AT][AT]GG
Tsp45I 565 GTCAC ^GT[GC]AC
Tsp509I 48 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 120 GGACC G^G[AT]CC
651 GGACC
BanII 289 GGGCCC G[GA]GC[CT]^C
301 GGGCTC
BcnI 14 CCCGG CC^[GC]GG
648 CCGGG
BmyI 289 GGGCCC G[AGT]GC[ACT]^C
301 GGGCTC
Bsp1286I 289 GGGCCC G[AGT]GC[ACT]^C
301 GGGCTC
DpnII 27 GATC ^GATC
417 GATC
EarI 853 CTCTTC CTCTTC(1/4)
387 GAAGAG
Ksp632I 853 CTCTTC CTCTTC(1/4)
387 GAAGAG
MboI 27 GATC ^GATC
417 GATC
NciI 14 CCCGG CC^[GC]GG
648 CCGGG
NdeII 27 GATC ^GATC
417 GATC
PflMI 99 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCACATCGTGG
PleI 744 GACTC GAGTC(4/5)
211 GAGTC
PpuMI 119 GGGACCT [GA]G^G[AT]CC[CT]
650 GGGACCT
PstI 436 CTGCAG CTGCA^G
520 CTGCAG
Sau3AI 27 GATC ^GATC
417 GATC
SfcI 436 CTGCAG C^T[GA][CT]AG
520 CTGCAG
TfiI 491 GATTC G^A[AT]TC
811 GATTC
Van91I 99 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCACATCGTGG
XcmI 113 CCATGAGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
473 CCAAGTTCGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwNI BbvI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstNI
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboII MnlI
MspA1I MspI MvaI MwoI NlaIII
NlaIV PvuII Sau96I ScrFI TaqI
TseI TspRI TthHB8I