Restriction map for
pFN21AB7236
(Linear) (Six-base) MAPSORT of: KIBB7236.seq Check: 7051 from: 1 to: 933
KIBB7236.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AfeI AGC'GCT
Cuts at: 0 94 933
Size: 94 839
AflIII A'CryG_T
Cuts at: 0 197 933
Size: 197 736
AlwNI CAG_nnn'CTG
Cuts at: 0 154 519 622 933
Size: 154 365 103 311
Fragments arranged by size:
365 311 154 103
ApaI G_GGCC'C
Cuts at: 0 293 933
Size: 293 640
BanII G_rGCy'C
Cuts at: 0 293 305 933
Size: 293 12 628
Fragments arranged by size:
628 293 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 479 933
Size: 445 34 454
Fragments arranged by size:
454 445 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 247 933
Size: 247 686
BclI T'GATC_A
Cuts at: 0 416 933
Size: 416 517
BlpI GC'TnA_GC
Cuts at: 0 304 933
Size: 304 629
Bme1580I G_kGCm'C
Cuts at: 0 293 933
Size: 293 640
BmgBI CAC'GTC
Cuts at: 0 198 255 711 933
Size: 198 57 456 222
Fragments arranged by size:
456 222 198 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 933
Size: 216 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 316 505 654 933
Size: 163 153 189 149 279
Fragments arranged by size:
279 189 163 153 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 933
Size: 625 308
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 405 841 862 933
Size: 405 436 21 71
Fragments arranged by size:
436 405 71 21
BseYI C'CCAG_C
Cuts at: 0 154 188 663 933
Size: 154 34 475 270
Fragments arranged by size:
475 270 154 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 622 933
Size: 222 400 311
Fragments arranged by size:
400 311 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 364 933
Size: 364 569
Bsp1286I G_dGCh'C
Cuts at: 0 293 305 933
Size: 293 12 628
Fragments arranged by size:
628 293 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 585 933
Size: 585 348
BstBI TT'CG_AA
Cuts at: 0 497 933
Size: 497 436
BstEII G'GTnAC_C
Cuts at: 0 564 933
Size: 564 369
BtgI C'CryG_G
Cuts at: 0 182 560 689 806 933
Size: 182 378 129 117 127
Fragments arranged by size:
378 182 129 127 117
BtsI GCAGTG_nn'
Cuts at: 0 71 210 429 933
Size: 71 139 219 504
Fragments arranged by size:
504 219 139 71
DraIII CAC_nnn'GTG
Cuts at: 0 411 933
Size: 411 522
EaeI y'GGCC_r
Cuts at: 0 2 107 925 933
Size: 2 105 818 8
Fragments arranged by size:
818 105 8 2
EarI CTCTTCn'nnn_
Cuts at: 0 382 859 933
Size: 382 477 74
Fragments arranged by size:
477 382 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 316 505 654 933
Size: 163 153 189 149 279
Fragments arranged by size:
279 189 163 153 149
EcoO109I rG'GnC_Cy
Cuts at: 0 120 289 290 556 651 933
Size: 120 169 1 266 95 282
Fragments arranged by size:
282 266 169 120 95 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 391 933
Size: 391 542
HaeII r_GCGC'y
Cuts at: 0 96 933
Size: 96 837
HincII GTy'rAC
Cuts at: 0 729 933
Size: 729 204
HindIII A'AGCT_T
Cuts at: 0 583 933
Size: 583 350
MscI TGG'CCA
Cuts at: 0 109 933
Size: 109 824
MslI CAynn'nnrTG
Cuts at: 0 412 933
Size: 412 521
MspA1I CmG'CkG
Cuts at: 0 7 151 192 233 516 789 860 933
Size: 7 144 41 41 283 273 71 73
Fragments arranged by size:
283 273 144 73 71 41 41 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 411 933
Size: 105 306 522
Fragments arranged by size:
522 306 105
PfoI T'CCnGG_A
Cuts at: 0 647 670 933
Size: 647 23 263
Fragments arranged by size:
647 263 23
PpuMI rG'GwC_Cy
Cuts at: 0 120 651 933
Size: 120 531 282
Fragments arranged by size:
531 282 120
PspOMI G'GGCC_C
Cuts at: 0 289 933
Size: 289 644
PstI C_TGCA'G
Cuts at: 0 440 524 933
Size: 440 84 409
Fragments arranged by size:
440 409 84
PvuII CAG'CTG
Cuts at: 0 151 192 233 516 933
Size: 151 41 41 283 417
Fragments arranged by size:
417 283 151 41 41
SbfI CC_TGCA'GG
Cuts at: 0 440 933
Size: 440 493
SexAI A'CCwGG_T
Cuts at: 0 98 933
Size: 98 835
SfcI C'TryA_G
Cuts at: 0 436 520 933
Size: 436 84 413
Fragments arranged by size:
436 413 84
StyI C'CwwG_G
Cuts at: 0 879 933
Size: 879 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 480 933
Size: 120 360 453
Fragments arranged by size:
453 360 120
Enzymes that do cut:
AfeI AflIII AlwNI ApaI BanII BcgI BciVI BclI
BlpI Bme1580I BmgBI BmrI BpmI BsaI BseRI BseYI
BsgI BsmBI Bsp1286I BspMI BstBI BstEII BtgI BtsI
DraIII EaeI EarI Eco57MI EcoO109I FalI HaeII HincII
HindIII MscI MslI MspA1I PflMI PfoI PpuMI PspOMI
PstI PvuII SbfI SexAI SfcI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI