(Circular) (Six-base) MAPSORT of: pFN21AB7233.seq Check: 9905 from: 1 to: 6052 pFN21AB7233 6052 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2180 3395 1937 Size: 243 1215 4594 Fragments arranged by size: 4594 1215 243 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2717 2922 4115 5499 5767 1632 Size: 1085 205 1193 1384 268 1917 Fragments arranged by size: 1917 1384 1193 1085 268 205 AfeI AGC'GCT Cuts at: 2594 2594 Size: 6052 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 3311 4883 3311 Size: 1572 4480 AhdI GACnn_n'nnGTC Cuts at: 2458 4781 2458 Size: 2323 3729 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2406 2930 1184 Size: 1222 524 4306 Fragments arranged by size: 4306 1222 524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2870 2871 2902 2903 2870 Size: 1 31 1 6019 Fragments arranged by size: 6019 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2205 3172 3795 5367 2205 Size: 967 623 1572 2890 Fragments arranged by size: 2890 1572 967 623 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2414 3377 1870 Size: 73 471 963 4545 Fragments arranged by size: 4545 963 471 73 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 3373 4828 618 Size: 325 217 627 310 1276 1455 1842 Fragments arranged by size: 1842 1455 1276 627 325 310 217 BanII G_rGCy'C Cuts at: 1391 2487 2799 2839 3371 1391 Size: 1096 312 40 532 4072 Fragments arranged by size: 4072 1096 532 312 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5081 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 4663 136 Size: 107 122 71 631 605 478 2513 1525 Fragments arranged by size: 2513 1525 631 605 478 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2450 4113 5269 1790 Size: 660 1663 1156 2573 Fragments arranged by size: 2573 1663 1156 660 BmgBI CAC'GTC Cuts at: 1318 3181 1318 Size: 1863 4189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2890 3783 4741 Size: 416 53 83 399 24 1473 893 958 Cuts at: 4741 442 Size: 1753 Fragments arranged by size: 1753 1473 958 893 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2728 2728 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 2381 2399 2420 2471 2603 2696 2987 Size: 840 302 18 21 51 132 93 291 Cuts at: 2987 3014 3164 3248 4712 1239 Size: 27 150 84 1464 2579 Fragments arranged by size: 2579 1464 840 302 291 150 132 93 84 51 27 21 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 4177 5042 5340 2702 Size: 1475 865 298 3414 Fragments arranged by size: 3414 1475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 2396 2852 3324 4715 882 Size: 617 816 81 456 472 1391 2219 Fragments arranged by size: 2219 1391 816 617 472 456 81 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2775 3216 3311 4485 4883 5157 5304 5715 2775 Size: 441 95 1174 398 274 147 411 3112 Fragments arranged by size: 3112 1174 441 411 398 274 147 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2038 2039 2068 2930 Size: 30 165 30 606 29 1 29 862 Cuts at: 2930 2960 1178 Size: 30 4270 Fragments arranged by size: 4270 862 606 165 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 2402 2434 2495 2717 2767 2951 1186 Size: 831 385 32 61 222 50 184 4287 Fragments arranged by size: 4287 831 385 222 184 61 50 32 BseYI C'CCAG_C Cuts at: 2198 2682 2761 3012 5255 5751 2198 Size: 484 79 251 2243 496 2499 Fragments arranged by size: 2499 2243 496 484 251 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2524 1163 Size: 573 788 4691 Fragments arranged by size: 4691 788 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2487 4113 4198 5269 2487 Size: 1626 85 1071 3270 Fragments arranged by size: 3270 1626 1071 85 BsmI GAATG_Cn' Cuts at: 2395 2524 2992 3563 3656 2395 Size: 129 468 571 93 4791 Fragments arranged by size: 4791 571 468 129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2450 2487 2799 2839 3371 4113 4198 Size: 399 660 37 312 40 532 742 85 Cuts at: 4198 5269 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 660 532 399 312 85 40 37 BspEI T'CCGG_A Cuts at: 2775 5715 2775 Size: 2940 3112 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3407 844 Size: 2563 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3148 3939 1394 Size: 429 1325 791 3507 Fragments arranged by size: 3507 1325 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3311 4696 4883 1700 Size: 110 1501 1385 187 2869 Fragments arranged by size: 2869 1501 1385 187 110 BsrGI T'GTAC_A Cuts at: 96 2533 96 Size: 2437 3615 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 2432 2729 4112 5124 2432 Size: 297 1383 1012 3360 Fragments arranged by size: 3360 1383 1012 297 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6052 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2359 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2496 2961 3129 3382 3749 4134 4151 5603 Size: 524 465 168 253 367 385 17 1452 Cuts at: 5603 5689 5701 6047 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 524 465 385 367 346 253 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2049 2850 3331 513 Size: 552 984 801 481 3234 Fragments arranged by size: 3234 984 801 552 481 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 2226 2951 3568 4362 4382 2226 Size: 725 617 794 20 3896 Fragments arranged by size: 3896 794 725 617 20 ClaI AT'CG_AT Cuts at: 1554 2088 1554 Size: 534 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DraIII CAC_nnn'GTG Cuts at: 2445 2445 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2308 2349 Size: 54 1425 50 275 18 312 166 41 Cuts at: 2349 2661 3328 3499 4388 6017 8 Size: 312 667 171 889 1629 43 Fragments arranged by size: 1629 1425 889 667 312 312 275 171 166 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3108 3982 1353 Size: 1755 874 3423 Fragments arranged by size: 3423 1755 874 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2633 4639 5013 5159 387 Size: 1064 138 1044 2006 374 146 1280 Fragments arranged by size: 2006 1280 1064 1044 374 146 138 EcoICRI GAG'CTC Cuts at: 2485 2485 Size: 6052 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2381 2399 2420 2471 2603 2696 Size: 393 447 302 18 21 51 132 93 Cuts at: 2696 2717 2922 2987 3014 3164 3248 4115 4712 Size: 21 205 65 27 150 84 867 597 Cuts at: 4712 5499 5767 1239 Size: 787 268 1524 Fragments arranged by size: 1524 867 787 597 447 393 302 268 205 150 132 93 84 65 51 27 21 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 2363 2663 2699 2363 Size: 300 36 5716 Fragments arranged by size: 5716 300 36 EcoO109I rG'GnC_Cy Cuts at: 2247 2283 2247 Size: 36 6016 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 2596 3007 5199 1678 Size: 918 411 2192 2531 Fragments arranged by size: 2531 2192 918 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2821 2853 3107 Size: 32 135 32 872 32 345 32 254 Cuts at: 3107 3139 4693 4725 4767 4799 1373 Size: 32 1554 32 42 32 2626 Fragments arranged by size: 2626 1554 872 345 254 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2181 3396 3642 669 Size: 988 281 243 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 243 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 3174 5166 5350 1305 Size: 1632 237 1992 184 2007 Fragments arranged by size: 2007 1992 1632 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2310 2351 2663 3330 Size: 54 1425 343 312 166 41 312 667 Cuts at: 3330 10 Size: 2732 Fragments arranged by size: 2732 1425 667 343 312 312 166 54 41 MslI CAynn'nnrTG Cuts at: 518 1166 2871 4010 4369 4528 518 Size: 648 1705 1139 359 159 2042 Fragments arranged by size: 2042 1705 1139 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2377 2608 2761 3468 4145 5293 5538 1606 Size: 771 231 153 707 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 707 677 245 231 153 NcoI C'CATG_G Cuts at: 513 1065 2049 2850 3331 513 Size: 552 984 801 481 3234 Fragments arranged by size: 3234 984 801 552 481 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 3410 4955 1132 Size: 97 2181 1545 2229 Fragments arranged by size: 2229 2181 1545 97 PasI CC'CwG_GG Cuts at: 1348 2188 1348 Size: 840 5212 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2107 2611 2875 3299 1108 Size: 999 504 264 424 3861 Fragments arranged by size: 3861 999 504 424 264 PfoI T'CCnGG_A Cuts at: 2056 2643 2964 2056 Size: 587 321 5144 Fragments arranged by size: 5144 587 321 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2247 Size: 6052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3146 3178 3146 Size: 32 6020 PstI C_TGCA'G Cuts at: 830 2919 2946 3039 3404 830 Size: 2089 27 93 365 3478 Fragments arranged by size: 3478 2089 365 93 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2377 2608 2761 2377 Size: 231 153 5668 Fragments arranged by size: 5668 231 153 SacI G_AGCT'C Cuts at: 2487 2487 Size: 6052 SalI G'TCGA_C Cuts at: 1936 2179 3394 1936 Size: 243 1215 4594 Fragments arranged by size: 4594 1215 243 SapI GCTCTTCn'nnn_ Cuts at: 3108 3108 Size: 6052 SbfI CC_TGCA'GG Cuts at: 2919 3404 2919 Size: 485 5567 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 3165 3165 Size: 6052 SfcI C'TryA_G Cuts at: 826 1045 2017 2915 2942 3035 3400 4535 5216 Size: 219 972 898 27 93 365 1135 681 Cuts at: 5216 5407 826 Size: 191 1471 Fragments arranged by size: 1471 1135 972 898 681 365 219 191 93 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 3311 1700 Size: 1611 4441 SmaI CCC'GGG Cuts at: 1872 3379 1872 Size: 1507 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 2717 4156 5057 5319 820 Size: 197 926 471 303 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 471 303 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 2049 2664 2850 3331 3491 513 Size: 552 984 615 186 481 160 3074 Fragments arranged by size: 3074 984 615 552 481 186 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 4079 4096 4249 4434 4879 325 Size: 1255 505 1994 17 153 185 445 1498 Fragments arranged by size: 1994 1498 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 3281 4298 Size: 274 80 33 51 1443 556 748 1017 Cuts at: 4298 6010 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1017 748 556 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2038 2070 2218 2250 2930 2962 6012 6044 2038 Size: 32 148 32 680 32 3050 32 2046 Fragments arranged by size: 3050 2046 680 148 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2137 2671 2137 Size: 534 5518 XhoI C'TCGA_G Cuts at: 1943 2414 1943 Size: 471 5581 XmaI C'CCGG_G Cuts at: 1870 3377 1870 Size: 1507 4545 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI