(Circular) (Six-base) MAPSORT of: pFN21AB7231.seq Check: 6266 from: 1 to: 6007 pFN21AB7231 6007 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2136 2136 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 2447 3328 2447 Size: 881 5126 AclI AA'CG_TT Cuts at: 1563 3164 4134 4507 1563 Size: 1601 970 373 3063 Fragments arranged by size: 3063 1601 970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 4070 5454 5722 1632 Size: 610 1828 1384 268 1917 Fragments arranged by size: 1917 1828 1384 610 268 AfeI AGC'GCT Cuts at: 2622 2622 Size: 6007 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 2181 4906 5883 1203 Size: 978 2725 977 1327 Fragments arranged by size: 2725 1327 978 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 3256 4736 3256 Size: 1480 4527 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4633 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2165 1184 Size: 981 5026 AlwNI CAG_nnn'CTG Cuts at: 3750 5322 3750 Size: 1572 4435 ApaI G_GGCC'C Cuts at: 2244 2244 Size: 6007 ApaLI G'TGCA_C Cuts at: 2150 4064 5220 2150 Size: 1914 1156 2937 Fragments arranged by size: 2937 1914 1156 ApoI r'AATT_y Cuts at: 1493 3221 3316 3542 3694 5803 1493 Size: 1728 95 226 152 2109 1697 Fragments arranged by size: 2109 1728 1697 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2236 3332 1870 Size: 73 293 1096 4545 Fragments arranged by size: 4545 1096 293 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2105 2216 2447 3328 Size: 325 217 627 267 51 111 231 881 Cuts at: 3328 4783 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 881 627 325 267 231 217 111 51 BanII G_rGCy'C Cuts at: 1391 2244 2550 2871 3326 1391 Size: 853 306 321 455 4072 Fragments arranged by size: 4072 853 455 321 306 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6007 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2248 928 Size: 971 349 4687 Fragments arranged by size: 4687 971 349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2248 4618 136 Size: 107 122 71 631 605 576 2370 1525 Fragments arranged by size: 2370 1525 631 605 576 122 107 71 BglII A'GATC_T Cuts at: 2466 6002 2466 Size: 3536 2471 BlpI GC'TnA_GC Cuts at: 2865 3424 2865 Size: 559 5448 Bme1580I G_kGCm'C Cuts at: 1790 2154 2219 2244 4068 5224 1790 Size: 364 65 25 1824 1156 2573 Fragments arranged by size: 2573 1824 1156 364 65 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2442 3117 Size: 416 53 83 399 24 897 128 675 Cuts at: 3117 3738 4696 442 Size: 621 958 1753 Fragments arranged by size: 1753 958 897 675 621 416 399 128 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2228 4667 1239 Size: 917 72 2439 2579 Fragments arranged by size: 2579 2439 917 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 4132 4997 5295 3119 Size: 1013 865 298 3831 Fragments arranged by size: 3831 1013 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 3148 4670 882 Size: 617 1092 557 1522 2219 Fragments arranged by size: 2219 1522 1092 617 557 BsaAI yAC'GTr Cuts at: 493 1204 2182 2718 3798 493 Size: 711 978 536 1080 2702 Fragments arranged by size: 2702 1080 978 711 536 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3814 4196 4831 Size: 53 83 186 936 522 1759 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1759 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3288 3291 3318 3321 1178 Size: 30 165 30 1885 3 27 3 3864 Fragments arranged by size: 3864 1885 165 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3312 3315 1186 Size: 1303 823 3 3878 Fragments arranged by size: 3878 1303 823 3 BseYI C'CCAG_C Cuts at: 2874 5210 5706 2874 Size: 2336 496 3175 Fragments arranged by size: 3175 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3291 3457 4218 5246 1540 Size: 275 186 928 362 166 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 362 275 186 166 BsiHKAI G_wGCw'C Cuts at: 2022 2154 2199 2871 4068 4153 5224 2022 Size: 132 45 672 1197 85 1071 2805 Fragments arranged by size: 2805 1197 1071 672 132 85 45 BsmI GAATG_Cn' Cuts at: 2749 3518 3611 2749 Size: 769 93 5145 Fragments arranged by size: 5145 769 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2154 2199 2219 2244 2550 2871 Size: 399 232 132 45 20 25 306 321 Cuts at: 2871 3326 4068 4153 5224 1391 Size: 455 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 455 399 321 306 232 132 85 45 25 20 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2166 2242 2760 3362 844 Size: 1292 30 76 518 602 3489 Fragments arranged by size: 3489 1292 602 518 76 30 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 2159 2198 4067 5079 2159 Size: 39 1869 1012 3087 Fragments arranged by size: 3087 1869 1012 39 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 2672 2710 1184 Size: 1488 38 4481 Fragments arranged by size: 4481 1488 38 BstXI CCAn_nnnn'nTGG Cuts at: 2773 2773 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2203 2466 3337 3704 4089 4106 5558 5644 Size: 231 263 871 367 385 17 1452 86 Cuts at: 5644 5656 6002 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 871 385 367 346 263 231 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2220 2538 3285 513 Size: 552 1048 107 318 747 3235 Fragments arranged by size: 3235 1048 747 552 318 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 2042 3523 4317 4337 2042 Size: 1481 794 20 3712 Fragments arranged by size: 3712 1481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 2689 2718 2689 Size: 29 5978 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 2926 3288 Size: 54 1425 50 275 18 933 163 362 Cuts at: 3288 3454 4343 5972 8 Size: 166 889 1629 43 Fragments arranged by size: 1629 1425 933 889 362 275 166 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 3288 3454 1537 Size: 275 1114 362 166 4090 Fragments arranged by size: 4090 1114 362 275 166 EarI CTCTTCn'nnn_ Cuts at: 1353 3937 1353 Size: 2584 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6007 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2228 2242 4070 4667 5454 5722 Size: 393 524 72 14 1828 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1828 1524 787 597 524 393 268 72 14 EcoO109I rG'GnC_Cy Cuts at: 2240 2241 2643 2240 Size: 1 402 5604 Fragments arranged by size: 5604 402 1 EcoRI G'AATT_C Cuts at: 3221 3316 3694 3221 Size: 95 378 5534 Fragments arranged by size: 5534 378 95 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2058 2624 5154 1678 Size: 380 566 2530 2531 Fragments arranged by size: 2531 2530 566 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3242 3274 4648 4680 4722 Size: 32 135 32 1670 32 1374 32 42 Cuts at: 4722 4754 1373 Size: 32 2626 Fragments arranged by size: 2626 1670 1374 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6007 KpnI G_GTAC'C Cuts at: 2451 3332 2451 Size: 881 5126 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5121 5305 1305 Size: 3816 184 2007 Fragments arranged by size: 3816 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2765 10 Size: 54 1425 343 933 3252 Fragments arranged by size: 3252 1425 933 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2540 2771 2969 3965 4324 4483 Size: 648 981 393 231 198 996 359 159 Cuts at: 4483 518 Size: 2042 Fragments arranged by size: 2042 996 981 648 393 359 231 198 159 MspA1I CmG'CkG Cuts at: 1606 2102 2134 2169 2407 2740 3423 4100 5248 Size: 496 32 35 238 333 683 677 1148 Cuts at: 5248 5493 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 683 677 496 333 245 238 35 32 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 2220 513 Size: 552 1155 4300 Fragments arranged by size: 4300 1155 552 NdeI CA'TA_TG Cuts at: 387 2633 387 Size: 2246 3761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2090 2968 3365 4910 1132 Size: 97 861 878 397 1545 2229 Fragments arranged by size: 2229 1545 878 861 397 97 PasI CC'CwG_GG Cuts at: 1348 2419 3078 1348 Size: 1071 659 4277 Fragments arranged by size: 4277 1071 659 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 1108 Size: 956 355 4696 Fragments arranged by size: 4696 956 355 PfoI T'CCnGG_A Cuts at: 2639 3126 2639 Size: 487 5520 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PmlI CAC'GTG Cuts at: 2718 2718 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2643 2643 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 2919 1613 Size: 1306 4701 PsiI TTA'TAA Cuts at: 2495 3577 2495 Size: 1082 4925 PspOMI G'GGCC_C Cuts at: 2240 2240 Size: 6007 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 5975 PstI C_TGCA'G Cuts at: 830 2133 2397 3359 830 Size: 1303 264 962 3478 Fragments arranged by size: 3478 1303 962 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2134 2407 2740 2134 Size: 273 333 5401 Fragments arranged by size: 5401 333 273 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 2397 3359 2397 Size: 962 5045 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SexAI A'CCwGG_T Cuts at: 2685 2685 Size: 6007 SfcI C'TryA_G Cuts at: 826 1045 2129 2393 3355 4490 5171 5362 826 Size: 219 1084 264 962 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 962 681 264 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6007 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 4111 5012 5274 820 Size: 197 926 1191 977 901 262 1553 Fragments arranged by size: 1553 1191 977 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 2968 3365 1132 Size: 1836 397 3774 Fragments arranged by size: 3774 1836 397 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 3179 1517 Size: 1662 4345 StyI C'CwwG_G Cuts at: 513 1065 2220 2310 2523 3446 513 Size: 552 1155 90 213 923 3074 Fragments arranged by size: 3074 1155 923 552 213 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2411 2677 2888 4034 4051 4204 4389 Size: 1255 831 266 211 1146 17 153 185 Cuts at: 4389 4834 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1146 831 445 266 211 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 2344 2360 2434 1316 Size: 1028 16 74 4889 Fragments arranged by size: 4889 1028 74 16 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 2977 2420 Size: 557 5450 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SapI SfiI SrfI SwaI