Restriction map for
pFN21AB7226
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 47 CGCG CG^CG
AlwNI 763 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 736 CCTGAGG CC^T[ACGT]AGG
BamHI 8 GGATCC G^GATCC
BanI 601 GGCACC G^G[CT][GA]CC
BanII 1267 GGGCTC G[GA]GC[CT]^C
BciVI 837 GGATAC GTATCC(6/5)
BlpI 1309 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1309 GCTGAGC GC^T[ACGT]AGC
BseRI 373 GAGGAG GAGGAG(10/8)
BsmAI 376 GAGAC GTCTC(1/5)
BsmI 1329 GAATGC GAATGC(1/-1)
BspHI 89 TCATGA T^CATGA
BspMI 440 ACCTGC ACCTGC(4/8)
BsrFI 356 ACCGGC [GA]^CCGG[CT]
BssSI 1105 CACGAG CACGAG(-5/-1)
BstUI 47 CGCG CG^CG
BstYI 8 GGATCC [GA]^GATC[CT]
Bsu36I 736 CCTGAGG CC^T[ACGT]AGG
CelII 1309 GCTGAGC GC^T[ACGT]AGC
CfoI 107 GCGC GCG^C
Cfr10I 356 ACCGGC [GA]^CCGG[CT]
DraII 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 110 CCATGG C^C[GA][CT]GG
Eco81I 736 CCTGAGG CC^T[ACGT]AGG
EcoO109I 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 106 AGCGCC [GA]GCGC^[CT]
HhaI 107 GCGC GCG^C
HinP1I 107 GCGC G^CGC
HincII 469 GTCAAC GT[CT]^[GA]AC
HindII 469 GTCAAC GT[CT]^[GA]AC
HindIII 703 AAGCTT A^AGCTT
HinfI 120 GAGTC G^A[ACGT]TC
MaeII 730 ACGT A^CGT
MflI 8 GGATCC [GA]^GATC[CT]
MseI 1198 TTAA T^TAA
MvnI 47 CGCG CG^CG
NcoI 110 CCATGG C^CATGG
NspI 452 ACATGC [GA]CATG^[CT]
PleI 120 GAGTC GAGTC(4/5)
PshAI 661 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 89 TCATGA T^CATGA
ScaI 1094 AGTACT AGT^ACT
SexAI 95 ACCAGGT A^CC[AT]GGT
StuI 381 AGGCCT AGG^CCT
Tru9I 1198 TTAA T^TAA
XcmI 869 CCACCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 8 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 244 GTAC GT^AC
1095 GTAC
Alw44I 1072 GTGCAC G^TGCAC
1102 GTGCAC
AlwI 9 GATCC GGATC(4/5)
8 GGATC
ApaLI 1072 GTGCAC G^TGCAC
1102 GTGCAC
AspHI 1072 GTGCAC G[AT]GC[AT]^C
1102 GTGCAC
BalI 1124 TGGCCA TGG^CCA
1289 TGGCCA
BbsI 432 GAAGAC GAAGAC(2/6)
975 GAAGAC
BfaI 446 CTAG C^TAG
1066 CTAG
BglI 593 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
715 GCCGGGGAGGC
BpuAI 432 GAAGAC GAAGAC(2/6)
975 GAAGAC
BsiHKAI 1072 GTGCAC G[AT]GC[AT]^C
1102 GTGCAC
BspDI 529 ATCGAT AT^CGAT
1170 ATCGAT
BstAPI 762 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
793 GCAGAGCTTGC
ClaI 529 ATCGAT AT^CGAT
1170 ATCGAT
EaeI 1124 TGGCCA [CT]^GGCC[GA]
1289 TGGCCA
EcoT14I 110 CCATGG C^C[AT][AT]GG
962 CCAAGG
FauI 523 CCCGC CCCGC(4/6)
366 GCGGG
MaeI 446 CTAG C^TAG
1066 CTAG
MluNI 1124 TGGCCA TGG^CCA
1289 TGGCCA
MscI 1124 TGGCCA TGG^CCA
1289 TGGCCA
MslI 276 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
582 CATGCCTGTG
RsaI 244 GTAC GT^AC
1095 GTAC
StyI 110 CCATGG C^C[AT][AT]GG
962 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I