Restriction map for
pFN21AB7226
(Linear) (Six-base) MAPSORT of: KIBB7226.seq Check: 1693 from: 1 to: 1359
KIBB7226.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 917 1105 1359
Size: 648 269 188 254
Fragments arranged by size:
648 269 254 188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 1359
Size: 1006 353
AlwNI CAG_nnn'CTG
Cuts at: 0 768 1359
Size: 768 591
ApaLI G'TGCA_C
Cuts at: 0 1072 1102 1359
Size: 1072 30 257
Fragments arranged by size:
1072 257 30
BamHI G'GATC_C
Cuts at: 0 8 1359
Size: 8 1351
BanI G'GyrC_C
Cuts at: 0 601 1359
Size: 601 758
BanII G_rGCy'C
Cuts at: 0 1271 1359
Size: 1271 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 439 982 1359
Size: 439 543 377
Fragments arranged by size:
543 439 377
BciVI GTATCCnnnnn_n'
Cuts at: 0 831 1359
Size: 831 528
BglI GCCn_nnn'nGGC
Cuts at: 0 599 721 1359
Size: 599 122 638
Fragments arranged by size:
638 599 122
BlpI GC'TnA_GC
Cuts at: 0 1310 1359
Size: 1310 49
Bme1580I G_kGCm'C
Cuts at: 0 66 604 1076 1106 1359
Size: 66 538 472 30 253
Fragments arranged by size:
538 472 253 66 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 1234 1359
Size: 1234 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1061 1359
Size: 1061 298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 269 1033 1359
Size: 151 118 764 326
Fragments arranged by size:
764 326 151 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 1359
Size: 388 971
BseYI C'CCAG_C
Cuts at: 0 774 1353 1359
Size: 774 579 6
Fragments arranged by size:
774 579 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 697 979 1359
Size: 478 219 282 380
Fragments arranged by size:
478 380 282 219
BsiHKAI G_wGCw'C
Cuts at: 0 1076 1106 1359
Size: 1076 30 253
Fragments arranged by size:
1076 253 30
BsmI GAATG_Cn'
Cuts at: 0 1335 1359
Size: 1335 24
Bsp1286I G_dGCh'C
Cuts at: 0 66 604 1076 1106 1271 1359
Size: 66 538 472 30 165 88
Fragments arranged by size:
538 472 165 88 66 30
BspHI T'CATG_A
Cuts at: 0 89 1359
Size: 89 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 1359
Size: 449 910
BsrFI r'CCGG_y
Cuts at: 0 356 1359
Size: 356 1003
BssSI C'ACGA_G
Cuts at: 0 1105 1359
Size: 1105 254
BstAPI GCAn_nnn'nTGC
Cuts at: 0 768 799 1359
Size: 768 31 560
Fragments arranged by size:
768 560 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 879 1134 1359
Size: 588 291 255 225
Fragments arranged by size:
588 291 255 225
BstYI r'GATC_y
Cuts at: 0 8 1359
Size: 8 1351
Bsu36I CC'TnA_GG
Cuts at: 0 737 1359
Size: 737 622
BtgI C'CryG_G
Cuts at: 0 110 1359
Size: 110 1249
BtsI GCAGTG_nn'
Cuts at: 0 64 1359
Size: 64 1295
ClaI AT'CG_AT
Cuts at: 0 530 1171 1359
Size: 530 641 188
Fragments arranged by size:
641 530 188
EaeI y'GGCC_r
Cuts at: 0 1124 1289 1359
Size: 1124 165 70
Fragments arranged by size:
1124 165 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1050 1359
Size: 1050 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 269 648 917 1033 1105 1359
Size: 151 118 379 269 116 72 254
Fragments arranged by size:
379 269 254 151 118 116 72
EcoO109I rG'GnC_Cy
Cuts at: 0 723 1359
Size: 723 636
HaeII r_GCGC'y
Cuts at: 0 110 1359
Size: 110 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 242 274 867 899 1359
Size: 242 32 593 32 460
Fragments arranged by size:
593 460 242 32 32
HincII GTy'rAC
Cuts at: 0 471 1359
Size: 471 888
HindIII A'AGCT_T
Cuts at: 0 703 1359
Size: 703 656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1359
Size: 678 681
MscI TGG'CCA
Cuts at: 0 1126 1291 1359
Size: 1126 165 68
Fragments arranged by size:
1126 165 68
MslI CAynn'nnrTG
Cuts at: 0 280 586 1359
Size: 280 306 773
Fragments arranged by size:
773 306 280
MspA1I CmG'CkG
Cuts at: 0 219 765 1116 1359
Size: 219 546 351 243
Fragments arranged by size:
546 351 243 219
NcoI C'CATG_G
Cuts at: 0 110 1359
Size: 110 1249
NspI r_CATG'y
Cuts at: 0 456 1359
Size: 456 903
PasI CC'CwG_GG
Cuts at: 0 58 508 1359
Size: 58 450 851
Fragments arranged by size:
851 450 58
PfoI T'CCnGG_A
Cuts at: 0 128 479 833 1156 1277 1359
Size: 128 351 354 323 121 82
Fragments arranged by size:
354 351 323 128 121 82
PshAI GACnn'nnGTC
Cuts at: 0 665 1359
Size: 665 694
PstI C_TGCA'G
Cuts at: 0 31 795 812 1121 1359
Size: 31 764 17 309 238
Fragments arranged by size:
764 309 238 31 17
PvuII CAG'CTG
Cuts at: 0 219 765 1116 1359
Size: 219 546 351 243
Fragments arranged by size:
546 351 243 219
ScaI AGT'ACT
Cuts at: 0 1096 1359
Size: 1096 263
SexAI A'CCwGG_T
Cuts at: 0 95 1359
Size: 95 1264
SfcI C'TryA_G
Cuts at: 0 27 791 808 905 1117 1359
Size: 27 764 17 97 212 242
Fragments arranged by size:
764 242 212 97 27 17
StuI AGG'CCT
Cuts at: 0 383 1359
Size: 383 976
StyI C'CwwG_G
Cuts at: 0 110 962 1359
Size: 110 852 397
Fragments arranged by size:
852 397 110
TatI w'GTAC_w
Cuts at: 0 1094 1359
Size: 1094 265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1359
Size: 876 483
Enzymes that do cut:
AcuI AlfI AlwNI ApaLI BamHI BanI BanII BbsI
BciVI BglI BlpI Bme1580I BmrI BplI BpmI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI
EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PasI PfoI
PshAI PstI PvuII ScaI SexAI SfcI StuI StyI
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI
BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI