Restriction map for
pFN21AB7225
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI ItaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TseI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 742 GAATTT [GA]^AATT[CT]
ApaI 470 GGGCCC GGGCC^C
ApoI 742 GAATTT [GA]^AATT[CT]
AseI 112 ATTAAT AT^TAAT
AsnI 112 ATTAAT AT^TAAT
AvaI 443 CTCGAG C^[CT]CG[GA]G
AvaII 510 GGACC G^G[AT]CC
BanII 470 GGGCCC G[GA]GC[CT]^C
BmyI 470 GGGCCC G[AGT]GC[ACT]^C
BsaJI 698 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 479 TCCGGT [AT]^CCGG[AT]
BsmI 595 GAATGC GAATGC(1/-1)
BsoBI 443 CTCGAG C^[CT]CG[GA]G
Bsp1286I 470 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 667 TGTACA T^GTACA
BsrDI 105 CATTGC GCAATG(2/0)
BsrGI 667 TGTACA T^GTACA
BssKI 297 CCAGG ^CC[ACGT]GG
BstNI 297 CCAGG CC^[AT]GG
BstYI 685 AGATCC [GA]^GATC[CT]
Cac8I 607 GCCAGC GC[ACGT]^[ACGT]GC
DdeI 263 CTGAG C^T[ACGT]AG
EarI 25 CTCTTC CTCTTC(1/4)
EcoRII 297 CCAGG ^CC[AT]GG
EcoT14I 698 CCAAGG C^C[AT][AT]GG
HapII 480 CCGG C^CGG
HincII 414 GTTGAC GT[CT]^[GA]AC
HindII 414 GTTGAC GT[CT]^[GA]AC
HpaII 480 CCGG C^CGG
HphI 18 GGTGA GGTGA(8/7)
Ksp632I 25 CTCTTC CTCTTC(1/4)
MaeII 633 ACGT A^CGT
MflI 685 AGATCC [GA]^GATC[CT]
MspA1I 609 CAGCTG C[AC]G^C[GT]G
MspI 480 CCGG C^CGG
MvaI 297 CCAGG CC^[AT]GG
MwoI 39 GCTTCTCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 290 CATATG CA^TATG
PaeR7I 443 CTCGAG C^TCGAG
PleI 637 GACTC GAGTC(4/5)
PshBI 112 ATTAAT AT^TAAT
PvuII 609 CAGCTG CAG^CTG
ScaI 266 AGTACT AGT^ACT
ScrFI 297 CCAGG CC^[ACGT]GG
SfaNI 175 GCATC GCATC(5/9)
SmlI 443 CTCGAG C^T[CT][GA]AG
SspBI 667 TGTACA T^GTACA
StyI 698 CCAAGG C^C[AT][AT]GG
TaqI 444 TCGA T^CGA
TthHB8I 444 TCGA T^CGA
XhoI 443 CTCGAG C^TCGAG
XhoII 685 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AlwI 343 GATCC GGATC(4/5)
686 GATCC
BsiYI 462 CCATGAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
473 CCCATCTCCGG
BslI 462 CCATGAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
473 CCCATCTCCGG
BsrI 66 ACTGG ACTGG(1/-1)
119 CCAGT
MnlI 442 CCTC CCTC(7/6)
446 GAGG
NlaIV 470 GGGCCC GG[ACGT]^[ACGT]CC
604 GGGGCC
Tsp45I 635 GTGAC ^GT[GC]AC
736 GTGAC
TspRI 65 CACTG CA[GC]TG[ACGT][ACGT]^
120 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI Cfr13I DpnII HaeIII
HinfI MaeIII MboI MboII MseI
NdeII NlaIII RsaI Sau3AI Sau96I
TfiI Tru9I Tsp509I