Restriction map for
pFN21AB7219
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BmyI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 24 GTCGAC GT^[AC][GT]AC
AciI 363 CCGC CCGC(-3/-1)
AlwI 111 GATCC GGATC(4/5)
AspI 597 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 415 GGTGCC G^G[CT][GA]CC
BbsI 817 GAAGAC GAAGAC(2/6)
BlpI 722 GCTCAGC GC^T[ACGT]AGC
BpmI 67 CTCCAG CTGGAG(16/14)
Bpu1102I 722 GCTCAGC GC^T[ACGT]AGC
BpuAI 817 GAAGAC GAAGAC(2/6)
BsmFI 232 GTCCC GGGAC(10/14)
BspHI 404 TCATGA T^CATGA
BsrDI 487 GCAATG GCAATG(2/0)
BstXI 248 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 247 GCCAGC GC[ACGT]^[ACGT]GC
CelII 722 GCTCAGC GC^T[ACGT]AGC
DraIII 327 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 24 GTCGAC GT[CT]^[GA]AC
HindII 24 GTCGAC GT[CT]^[GA]AC
MslI 168 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 434 CAGCTG C[AC]G^C[GT]G
PflFI 597 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 434 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SalI 24 GTCGAC G^TCGAC
SfcI 885 CTATAG C^T[GA][CT]AG
StuI 707 AGGCCT AGG^CCT
Tsp45I 448 GTCAC ^GT[GC]AC
Tth111I 597 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
AfaI 102 GTAC GT^AC
774 GTAC
ApoI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
BalI 371 TGGCCA TGG^CCA
425 TGGCCA
BbvI 433 GCAGC GCAGC(8/12)
913 GCTGC
BglI 316 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
418 GCCTATGTGGC
BsgI 163 CTGCAC GTGCAG(16/14)
472 GTGCAG
BspMI 161 ACCTGC ACCTGC(4/8)
718 ACCTGC
BssKI 39 CCTGG ^CC[ACGT]GG
443 CCTGG
BstF5I 98 GGATG GGATG(2/0)
903 GGATG
BstNI 39 CCTGG CC^[AT]GG
443 CCTGG
DpnII 111 GATC ^GATC
339 GATC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
830 AGGCCCT
EaeI 371 TGGCCA [CT]^GGCC[GA]
425 TGGCCA
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
830 AGGCCCT
EcoRII 39 CCTGG ^CC[AT]GG
443 CCTGG
EcoT14I 317 CCATGG C^C[AT][AT]GG
428 CCATGG
Fnu4HI 433 GCAGC GC^[ACGT]GC
913 GCTGC
FokI 98 GGATG GGATG(9/13)
903 GGATG
HphI 741 GGTGA GGTGA(8/7)
159 TCACC
ItaI 433 GCAGC GC^[ACGT]GC
913 GCTGC
MaeII 22 ACGT A^CGT
209 ACGT
MaeIII 448 GTCAC ^GT[ACGT]AC
495 GTTAC
MboI 111 GATC ^GATC
339 GATC
MluNI 371 TGGCCA TGG^CCA
425 TGGCCA
MscI 371 TGGCCA TGG^CCA
425 TGGCCA
MseI 521 TTAA T^TAA
872 TTAA
MvaI 39 CCTGG CC^[AT]GG
443 CCTGG
NcoI 317 CCATGG C^CATGG
428 CCATGG
NdeII 111 GATC ^GATC
339 GATC
RsaI 102 GTAC GT^AC
774 GTAC
Sau3AI 111 GATC ^GATC
339 GATC
ScrFI 39 CCTGG CC^[ACGT]GG
443 CCTGG
StyI 317 CCATGG C^C[AT][AT]GG
428 CCATGG
Tru9I 521 TTAA T^TAA
872 TTAA
TseI 433 GCAGC G^C[AT]GC
913 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AvaII BsaJI BsiYI BslI
BsrI Cfr13I DdeI DsaI HaeIII
HinfI MboII MnlI MwoI NlaIII
NlaIV PleI Sau96I TaqI TfiI
Tsp509I TspRI TthHB8I