(Circular) (Six-base) MAPSORT of: pFN21AB7218.seq Check: 84 from: 1 to: 5455 pFN21AB7218 5455 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2703 2798 1937 Size: 766 95 4594 Fragments arranged by size: 4594 766 95 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 2754 3582 3955 1563 Size: 1191 828 373 3063 Fragments arranged by size: 3063 1191 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 4354 5331 1203 Size: 3151 977 1327 Fragments arranged by size: 3151 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2491 1166 Size: 1325 4130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2249 2281 2249 Size: 32 5423 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaI G_GGCC'C Cuts at: 2624 2624 Size: 5455 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2764 2990 3142 5251 1493 Size: 1271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1271 226 152 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2780 1870 Size: 73 837 4545 Fragments arranged by size: 4545 837 73 BamHI G'GATC_C Cuts at: 2733 2785 3152 2733 Size: 52 367 5036 Fragments arranged by size: 5036 367 52 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2691 2776 4231 618 Size: 325 217 627 904 85 1455 1842 Fragments arranged by size: 1842 1455 904 627 325 217 85 BanII G_rGCy'C Cuts at: 1391 2624 2676 2774 1391 Size: 1233 52 98 4072 Fragments arranged by size: 4072 1233 98 52 BbeI G_GCGC'C Cuts at: 2695 2695 Size: 5455 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BbvCI CC'TCA_GC Cuts at: 2312 2399 2312 Size: 87 5368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2583 2617 3646 3680 1903 Size: 34 646 34 1029 34 3678 Fragments arranged by size: 3678 1029 646 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 4066 136 Size: 107 122 71 631 605 406 1988 1525 Fragments arranged by size: 1988 1525 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2670 2872 2670 Size: 202 5253 Bme1580I G_kGCm'C Cuts at: 1790 2624 3516 4672 1790 Size: 834 892 1156 2573 Fragments arranged by size: 2573 1156 892 834 BmgBI CAC'GTC Cuts at: 1318 2369 1318 Size: 1051 4404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2542 3186 4144 Size: 416 53 83 399 24 1125 644 958 Cuts at: 4144 442 Size: 1753 Fragments arranged by size: 1753 1125 958 644 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 2665 4115 1239 Size: 1295 131 1450 2579 Fragments arranged by size: 2579 1450 1295 131 Bpu10I CC'TnA_GC Cuts at: 2312 2399 2312 Size: 87 5368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3580 4445 4743 3580 Size: 865 298 4292 Fragments arranged by size: 4292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4118 882 Size: 617 2619 2219 Fragments arranged by size: 2619 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2692 3262 3644 4279 Size: 53 83 186 936 1159 570 382 635 Cuts at: 4279 275 Size: 1451 Fragments arranged by size: 1451 1159 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2254 3888 4286 4560 4707 5118 2254 Size: 1634 398 274 147 411 2591 Fragments arranged by size: 2591 1634 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2249 2279 1178 Size: 30 165 30 846 30 4354 Fragments arranged by size: 4354 846 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2009 1186 Size: 823 4632 BseYI C'CCAG_C Cuts at: 2011 2055 2103 2445 4658 5154 2011 Size: 44 48 342 2213 496 2312 Fragments arranged by size: 2312 2213 496 342 48 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 1163 Size: 573 669 4213 Fragments arranged by size: 4213 669 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2029 2232 3516 3601 4672 2029 Size: 203 1284 85 1071 2812 Fragments arranged by size: 2812 1284 1071 203 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2232 2624 2676 2774 3516 3601 Size: 399 239 203 392 52 98 742 85 Cuts at: 3601 4672 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 239 203 98 85 52 BspEI T'CCGG_A Cuts at: 2254 5118 2254 Size: 2864 2591 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2641 3950 4124 65 Size: 1183 1393 1309 174 1396 Fragments arranged by size: 1396 1393 1309 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 4099 4286 1700 Size: 110 307 1982 187 2869 Fragments arranged by size: 2869 1982 307 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2733 2785 3152 3537 3554 5006 5092 5104 Size: 761 52 367 385 17 1452 86 12 Cuts at: 5104 5450 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 761 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4903 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2191 2435 2486 2498 2971 3765 3785 2191 Size: 244 51 12 473 794 20 3861 Fragments arranged by size: 3861 794 473 244 51 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420 Size: 54 1425 50 275 18 1072 889 1629 Cuts at: 5420 8 Size: 43 Fragments arranged by size: 1629 1425 1072 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2147 3385 1353 Size: 776 18 1238 3423 Fragments arranged by size: 3423 1238 776 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2207 2339 4042 4416 4562 387 Size: 1064 138 618 132 1703 374 146 1280 Fragments arranged by size: 1703 1280 1064 618 374 146 138 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 2665 3518 4115 4902 5170 1239 Size: 393 902 131 853 597 787 268 1524 Fragments arranged by size: 1524 902 853 787 597 393 268 131 EcoNI CCTnn'n_nnAGG Cuts at: 2582 2582 Size: 5455 EcoO109I rG'GnC_Cy Cuts at: 2308 2620 2621 2308 Size: 312 1 5142 Fragments arranged by size: 5142 312 1 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FseI GG_CCGG'CC Cuts at: 2121 2121 Size: 5455 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2319 2474 2695 4602 1678 Size: 641 155 221 1907 2531 Fragments arranged by size: 2531 1907 641 221 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373 Size: 32 135 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KasI G'GCGC_C Cuts at: 2691 2691 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3633 Fragments arranged by size: 3633 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 2491 3413 3772 3931 518 Size: 648 1165 160 922 359 159 2042 Fragments arranged by size: 2042 1165 922 648 359 160 159 MspA1I CmG'CkG Cuts at: 1606 2172 2186 2263 2871 3548 4696 4941 1606 Size: 566 14 77 608 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 566 245 77 14 NaeI GCC'GGC Cuts at: 2119 2119 Size: 5455 NarI GG'CG_CC Cuts at: 2692 2692 Size: 5455 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4903 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NgoMIV G'CCGG_C Cuts at: 2117 2117 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2428 2566 2813 4358 1132 Size: 97 1199 138 247 1545 2229 Fragments arranged by size: 2229 1545 1199 247 138 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5455 PciI A'CATG_T Cuts at: 4354 4354 Size: 5455 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5455 PfoI T'CCnGG_A Cuts at: 2511 2511 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2249 2281 3524 3556 5037 5069 2249 Size: 32 1243 32 1481 32 2635 Fragments arranged by size: 2635 1481 1243 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 5455 PshAI GACnn'nnGTC Cuts at: 1613 2411 1613 Size: 798 4657 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PspOMI G'GGCC_C Cuts at: 2620 2620 Size: 5455 PstI C_TGCA'G Cuts at: 830 2493 2807 830 Size: 1663 314 3478 Fragments arranged by size: 3478 1663 314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 PvuII CAG'CTG Cuts at: 2186 2263 2186 Size: 77 5378 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SapI GCTCTTCn'nnn_ Cuts at: 2147 2147 Size: 5455 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2489 2803 3938 4619 4810 826 Size: 219 1444 314 1135 681 191 1471 Fragments arranged by size: 1471 1444 1135 681 314 219 191 SfoI GGC'GCC Cuts at: 2693 2693 Size: 5455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2782 1872 Size: 910 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3559 4460 4722 820 Size: 197 926 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2428 2566 2813 1132 Size: 1296 138 247 3774 Fragments arranged by size: 3774 1296 247 138 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2108 2221 2466 2643 2894 513 Size: 552 1043 113 245 177 251 3074 Fragments arranged by size: 3074 1043 552 251 245 177 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3701 5413 96 Size: 274 80 33 51 1443 1724 1712 138 Fragments arranged by size: 1724 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2612 2612 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2780 1870 Size: 910 4545 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI