Restriction map for
pFN21AB7215
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# NO CUT ENZYMES ( 208 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AfaI 115 GTAC GT^AC
AlwI 133 GATCC GGATC(4/5)
BsaJI 50 CCCTGG C^C[ACGT][ACGT]GG
BsgI 190 CTGCAC GTGCAG(16/14)
BsiWI 114 CGTACG C^GTACG
BsmAI 33 GTCTC GTCTC(1/5)
BspMI 188 ACCTGC ACCTGC(4/8)
DpnII 133 GATC ^GATC
HinfI 213 GATTC G^A[ACGT]TC
MaeII 113 ACGT A^CGT
MboI 133 GATC ^GATC
MseI 71 TTAA T^TAA
MspA1I 104 CAGCTG C[AC]G^C[GT]G
NdeII 133 GATC ^GATC
NlaIII 27 CATG CATG^
PvuII 104 CAGCTG CAG^CTG
RsaI 115 GTAC GT^AC
Sau3AI 133 GATC ^GATC
SexAI 316 ACCAGGT A^CC[AT]GGT
TfiI 213 GATTC G^A[AT]TC
Tru9I 71 TTAA T^TAA
XcmI 8 CCAGCAGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 13 AGCT AG^CT
105 AGCT
AvaII 5 GGTCC G^G[AT]CC
21 GGTCC
BbvI 11 GCAGC GCAGC(8/12)
119 GCTGC
BseRI 306 CTCCTC GAGGAG(10/8)
309 CTCCTC
BsiYI 274 CCTGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
311 CCTCTACCAGG
BslI 274 CCTGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
311 CCTCTACCAGG
BsrI 228 ACTGG ACTGG(1/-1)
141 CCAGT
BstF5I 249 GGATG GGATG(2/0)
283 GGATG
Cfr13I 5 GGTCC G^G[ACGT]CC
21 GGTCC
DdeI 275 CTGAG C^T[ACGT]AG
326 CTCAG
EcoNI 274 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
311 CCTCTACCAGG
Fnu4HI 11 GCAGC GC^[ACGT]GC
119 GCTGC
FokI 249 GGATG GGATG(9/13)
283 GGATG
HaeIII 266 GGCC GG^CC
291 GGCC
ItaI 11 GCAGC GC^[ACGT]GC
119 GCTGC
MboII 232 GAAGA GAAGA(8/7)
253 GAAGA
NlaIV 4 GGGTCC GG[ACGT]^[ACGT]CC
304 GGCTCC
Sau96I 5 GGTCC G^G[ACGT]CC
21 GGTCC
TseI 11 GCAGC G^C[AT]GC
119 GCTGC
Tsp45I 46 GTGAC ^GT[GC]AC
146 GTCAC
TspRI 142 CAGTG CA[GC]TG[ACGT][ACGT]^
193 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BssKI BstNI EcoRII MaeIII MnlI
MvaI ScrFI