Restriction map for
pFN21AB7209
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MflI MluI MluNI
MscI MseI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 74 TCCGGA T^CCGGA
AlwNI 412 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 339 GAGCTC G[AT]GC[AT]^C
BbsI 100 GAAGAC GAAGAC(2/6)
BclI 170 TGATCA T^GATCA
BlpI 517 GCTGAGC GC^T[ACGT]AGC
BpmI 334 CTCCAG CTGGAG(16/14)
Bpu1102I 517 GCTGAGC GC^T[ACGT]AGC
BpuAI 100 GAAGAC GAAGAC(2/6)
BsaBI 171 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 74 TCCGGA [AT]^CCGG[AT]
BseAI 74 TCCGGA T^CCGGA
BsiHKAI 339 GAGCTC G[AT]GC[AT]^C
BspEI 74 TCCGGA T^CCGGA
CelII 517 GCTGAGC GC^T[ACGT]AGC
CfoI 525 GCGC GCG^C
DpnII 171 GATC ^GATC
DrdI 475 GACCCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 479 CCATGG C^C[GA][CT]GG
EcoNI 106 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 479 CCATGG C^C[AT][AT]GG
EcoT22I 117 ATGCAT ATGCA^T
FbaI 170 TGATCA T^GATCA
HhaI 525 GCGC GCG^C
HinP1I 525 GCGC G^CGC
MaeII 79 ACGT A^CGT
MaeIII 484 GTCAC ^GT[ACGT]AC
MamI 171 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 171 GATC ^GATC
MroI 74 TCCGGA T^CCGGA
MslI 155 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 237 CCGCTG C[AC]G^C[GT]G
NcoI 479 CCATGG C^CATGG
NdeI 10 CATATG CA^TATG
NdeII 171 GATC ^GATC
NsiI 117 ATGCAT ATGCA^T
NspI 530 GCATGT [GA]CATG^[CT]
SacI 339 GAGCTC GAGCT^C
Sau3AI 171 GATC ^GATC
ScaI 272 AGTACT AGT^ACT
SfaNI 116 GATGC GCATC(5/9)
SfcI 110 CTGTAG C^T[GA][CT]AG
StyI 479 CCATGG C^C[AT][AT]GG
TaqI 210 TCGA T^CGA
TfiI 396 GATTC G^A[AT]TC
Tsp45I 484 GTCAC ^GT[GC]AC
Tsp509I 442 AATT ^AATT
TthHB8I 210 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 237 CCGC CCGC(-3/-1)
528 CCGC
AvaII 6 GGTCC G^G[AT]CC
474 GGACC
BanII 339 GAGCTC G[GA]GC[CT]^C
352 GAGCCC
BmyI 339 GAGCTC G[AGT]GC[ACT]^C
352 GAGCCC
BsmFI 454 GGGAC GGGAC(10/14)
492 GTCCC
Bsp1286I 339 GAGCTC G[AGT]GC[ACT]^C
352 GAGCCC
BsrI 275 ACTGG ACTGG(1/-1)
288 CCAGT
DdeI 129 CTCAG C^T[ACGT]AG
518 CTGAG
DraII 364 AGGCCCC [GA]G^G[ACGT]CC[CT]
413 AGGCCCT
EcoO109I 364 AGGCCCC [GA]G^G[ACGT]CC[CT]
413 AGGCCCT
HapII 75 CCGG C^CGG
349 CCGG
HpaII 75 CCGG C^CGG
349 CCGG
MboII 100 GAAGA GAAGA(8/7)
567 TCTTC
MspI 75 CCGG C^CGG
349 CCGG
MwoI 83 GCCACTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
119 GCATTTCAGGC
PleI 388 GACTC GAGTC(4/5)
581 GACTC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MnlI MvaI NlaIII
NlaIV RsaI Sau96I ScrFI TseI
TspRI