(Circular) (Six-base) MAPSORT of: pFN21AB7206.seq Check: 5602 from: 1 to: 6430 pFN21AB7206 6430 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2865 4240 278 Size: 53 83 186 936 1329 1375 2468 Fragments arranged by size: 2468 1375 1329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2628 3773 1937 Size: 691 1145 4594 Fragments arranged by size: 4594 1145 691 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4493 5877 6145 1632 Size: 2861 1384 268 1917 Fragments arranged by size: 2861 1917 1384 268 AfeI AGC'GCT Cuts at: 3375 3375 Size: 6430 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 3699 5329 6306 1203 Size: 2496 1630 977 1327 Fragments arranged by size: 2496 1630 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 2648 1166 Size: 1482 4948 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2037 1184 Size: 853 5577 AlwNI CAG_nnn'CTG Cuts at: 2315 3337 4173 5745 2315 Size: 1022 836 1572 3000 Fragments arranged by size: 3000 1572 1022 836 ApaI G_GGCC'C Cuts at: 2390 2390 Size: 6430 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 3008 3464 3739 3965 4117 6226 1493 Size: 1515 456 275 226 152 2109 1697 Fragments arranged by size: 2109 1697 1515 456 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2294 2592 3302 3755 1870 Size: 73 351 298 710 453 4545 Fragments arranged by size: 4545 710 453 351 298 73 AvrII C'CTAG_G Cuts at: 2782 2782 Size: 6430 BamHI G'GATC_C Cuts at: 3327 3760 4127 3327 Size: 433 367 5630 Fragments arranged by size: 5630 433 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 3751 5206 618 Size: 325 217 627 260 1704 1455 1842 Fragments arranged by size: 1842 1704 1455 627 325 260 217 BanII G_rGCy'C Cuts at: 1391 2390 3173 3274 3749 1391 Size: 999 783 101 475 4072 Fragments arranged by size: 4072 999 783 475 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3530 928 Size: 971 1631 3828 Fragments arranged by size: 3828 1631 971 BbvCI CC'TCA_GC Cuts at: 2540 2540 Size: 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2156 2190 4621 4655 1903 Size: 34 50 34 135 34 2431 34 3678 Fragments arranged by size: 3678 2431 135 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BfrBI ATG'CAT Cuts at: 2273 2273 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2289 2378 2451 Size: 107 122 71 631 605 617 89 73 Cuts at: 2451 2460 5041 136 Size: 9 2581 1525 Fragments arranged by size: 2581 1525 631 617 605 122 107 89 73 71 9 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2178 3722 3847 2178 Size: 1544 125 4761 Fragments arranged by size: 4761 1544 125 Bme1580I G_kGCm'C Cuts at: 1790 2052 2390 2408 4491 5647 1790 Size: 262 338 18 2083 1156 2573 Fragments arranged by size: 2573 2083 1156 338 262 18 BmgBI CAC'GTC Cuts at: 1318 3454 3700 1318 Size: 2136 246 4048 Fragments arranged by size: 4048 2136 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2385 3275 Size: 416 53 83 399 24 682 286 890 Cuts at: 3275 4161 5119 442 Size: 886 958 1753 Fragments arranged by size: 1753 958 890 886 682 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2489 2834 3074 3515 2489 Size: 345 240 441 5404 Fragments arranged by size: 5404 441 345 240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2370 2385 2430 2968 3035 3069 3449 3539 Size: 1131 15 45 538 67 34 380 90 Cuts at: 3539 5090 1239 Size: 1551 2579 Fragments arranged by size: 2579 1551 1131 538 380 90 67 45 34 15 Bpu10I CC'TnA_GC Cuts at: 2540 2633 2540 Size: 93 6337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 4555 5420 5718 2234 Size: 2321 865 298 2946 Fragments arranged by size: 2946 2321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2153 2477 3415 5093 882 Size: 617 654 324 938 1678 2219 Fragments arranged by size: 2219 1678 938 654 617 324 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2862 3648 4237 4619 Size: 53 83 186 936 1329 786 589 382 Cuts at: 4619 5254 275 Size: 635 1451 Fragments arranged by size: 1451 1329 936 786 635 589 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2493 2523 2966 2996 1178 Size: 30 165 30 1090 30 443 30 4612 Fragments arranged by size: 4612 1090 443 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2514 3066 1186 Size: 1328 552 4550 Fragments arranged by size: 4550 1328 552 BseYI C'CCAG_C Cuts at: 2182 2401 2902 2923 3141 3414 3637 5633 6129 Size: 219 501 21 218 273 223 1996 496 Cuts at: 6129 2182 Size: 2483 Fragments arranged by size: 2483 1996 501 496 273 223 219 218 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5857 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 4491 4576 5647 2523 Size: 1968 85 1071 3306 Fragments arranged by size: 3306 1968 1071 85 BsmI GAATG_Cn' Cuts at: 2275 3611 3941 4034 2275 Size: 1336 330 93 4671 Fragments arranged by size: 4671 1336 330 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2390 2408 2523 3173 3274 3749 Size: 399 262 338 18 115 650 101 475 Cuts at: 3749 4491 4576 5647 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 650 475 399 338 262 115 101 85 18 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 3501 4319 6049 3501 Size: 818 1730 3882 Fragments arranged by size: 3882 1730 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3785 844 Size: 2941 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3630 3691 4925 5099 65 Size: 1183 2382 61 1234 174 1396 Fragments arranged by size: 2382 1396 1234 1183 174 61 BsrFI r'CCGG_y Cuts at: 1700 1810 2331 5074 5261 1700 Size: 110 521 2743 187 2869 Fragments arranged by size: 2869 2743 521 187 110 BsrGI T'GTAC_A Cuts at: 96 2640 96 Size: 2544 3886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 2828 2930 4490 5502 2828 Size: 102 1560 1012 3756 Fragments arranged by size: 3756 1560 1012 102 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 2830 3078 3166 3427 2830 Size: 248 88 261 5833 Fragments arranged by size: 5833 261 248 88 BstYI r'GATC_y Cuts at: 1972 2208 3327 3760 4127 4512 4529 5981 6067 Size: 236 1119 433 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1119 433 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2730 3120 513 Size: 552 1665 390 3823 Fragments arranged by size: 3823 1665 552 390 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2674 2782 487 Size: 683 56 1448 108 4135 Fragments arranged by size: 4135 1448 683 108 56 BtsI GCAGTG_nn' Cuts at: 2172 2266 3393 3946 4740 4760 2172 Size: 94 1127 553 794 20 3842 Fragments arranged by size: 3842 1127 794 553 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2087 5437 809 Size: 507 771 3350 1802 Fragments arranged by size: 3350 1802 771 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 2856 3080 Size: 54 1425 50 275 18 822 204 224 Cuts at: 3080 3147 3390 3877 4766 6395 8 Size: 67 243 487 889 1629 43 Fragments arranged by size: 1629 1425 889 822 487 275 243 224 204 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 3231 4360 1353 Size: 1307 571 1129 3423 Fragments arranged by size: 3423 1307 1129 571 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2478 5017 5391 5537 387 Size: 1064 138 889 2539 374 146 1280 Fragments arranged by size: 2539 1280 1064 889 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2370 2385 2430 2968 3035 3069 3449 Size: 393 738 15 45 538 67 34 380 Cuts at: 3449 3539 4493 5090 5877 6145 1239 Size: 90 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 738 597 538 393 380 268 90 67 45 34 15 EcoNI CCTnn'n_nnAGG Cuts at: 2042 3578 2042 Size: 1536 4894 EcoO109I rG'GnC_Cy Cuts at: 2096 2236 2290 3709 2096 Size: 140 54 1419 4817 Fragments arranged by size: 4817 1419 140 54 EcoRI G'AATT_C Cuts at: 3008 3464 3739 4117 3008 Size: 456 275 378 5321 Fragments arranged by size: 5321 456 378 275 EcoRV GAT'ATC Cuts at: 3217 3217 Size: 6430 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 3377 5577 1678 Size: 1699 2200 2531 Fragments arranged by size: 2531 2200 1699 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2493 2525 2575 2607 3045 Size: 32 135 32 921 32 50 32 438 Cuts at: 3045 3077 3261 3293 5071 5103 5145 5177 1373 Size: 32 184 32 1778 32 42 32 2626 Fragments arranged by size: 2626 1778 921 438 184 135 50 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3684 5544 5728 1305 Size: 2379 1860 184 2007 Fragments arranged by size: 2379 2007 1860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 3082 3149 3392 10 Size: 54 1425 343 822 428 67 243 3048 Fragments arranged by size: 3048 1425 822 428 343 243 67 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 3076 3452 3581 3698 4388 4747 Size: 648 1482 428 376 129 117 690 359 Cuts at: 4747 4906 518 Size: 159 2042 Fragments arranged by size: 2042 1482 690 648 428 376 359 159 129 117 MspA1I CmG'CkG Cuts at: 1606 2182 2426 2923 3086 3145 3846 4523 5671 Size: 576 244 497 163 59 701 677 1148 Cuts at: 5671 5916 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 701 677 576 497 245 244 163 59 NcoI C'CATG_G Cuts at: 513 1065 2730 3120 513 Size: 552 1665 390 3823 Fragments arranged by size: 3823 1665 552 390 NdeI CA'TA_TG Cuts at: 387 2275 387 Size: 1888 4542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NsiI A_TGCA'T Cuts at: 2275 2275 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2419 3203 3232 3788 5333 1132 Size: 97 1190 784 29 556 1545 2229 Fragments arranged by size: 2229 1545 1190 784 556 97 29 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6430 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6430 PfoI T'CCnGG_A Cuts at: 2682 3012 2682 Size: 330 6100 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2236 3709 2096 Size: 140 1473 4817 Fragments arranged by size: 4817 1473 140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2386 2386 Size: 6430 PstI C_TGCA'G Cuts at: 830 2013 2346 3192 3363 3782 830 Size: 1183 333 846 171 419 3478 Fragments arranged by size: 3478 1183 846 419 333 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2182 2426 2923 3086 3145 2182 Size: 244 497 163 59 5467 Fragments arranged by size: 5467 497 244 163 59 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SapI GCTCTTCn'nnn_ Cuts at: 3231 3231 Size: 6430 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2009 2342 3188 3359 3778 4913 5594 Size: 219 964 333 846 171 419 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 1471 1135 964 846 681 419 333 219 191 171 SfiI GGCCn_nnn'nGGCC Cuts at: 2289 2451 2289 Size: 162 6268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 2331 1700 Size: 631 5799 SmaI CCC'GGG Cuts at: 1872 3757 1872 Size: 1885 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 4534 5435 5697 820 Size: 197 926 270 2321 901 262 1553 Fragments arranged by size: 2321 1553 926 901 270 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 2419 3203 3788 1132 Size: 1287 784 585 3774 Fragments arranged by size: 3774 1287 784 585 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2052 2446 2476 2715 2730 2782 3120 Size: 552 987 394 30 239 15 52 338 Cuts at: 3120 3282 3869 513 Size: 162 587 3074 Fragments arranged by size: 3074 987 587 552 394 338 239 162 52 30 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2612 3113 4457 4474 4627 4812 Size: 1255 465 567 501 1344 17 153 185 Cuts at: 4812 5257 325 Size: 445 1498 Fragments arranged by size: 1498 1344 1255 567 501 465 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2640 2973 4676 Size: 274 80 33 51 1443 663 333 1703 Cuts at: 4676 6388 96 Size: 1712 138 Fragments arranged by size: 1712 1703 1443 663 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2731 2763 6390 6422 2731 Size: 32 3627 32 2739 Fragments arranged by size: 3627 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3205 3766 3205 Size: 561 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2893 3142 3262 2380 Size: 513 249 120 5548 Fragments arranged by size: 5548 513 249 120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 3755 1870 Size: 1885 4545 XmnI GAAnn'nnTTC Cuts at: 3240 4559 3240 Size: 1319 5111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2863 4238 276 Size: 53 83 186 936 1329 1375 2468 Fragments arranged by size: 2468 1375 1329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI