Restriction map for
pFN21AB7205
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsgI BsiWI BspDI BspLU11I BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MseI MunI MvnI NarI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Alw44I 421 GTGCAC G^TGCAC
AocI 252 CCTGAGG CC^T[ACGT]AGG
ApaLI 421 GTGCAC G^TGCAC
AspHI 421 GTGCAC G[AT]GC[AT]^C
BalI 187 TGGCCA TGG^CCA
BamHI 779 GGATCC G^GATCC
BbrPI 733 CACGTG CAC^GTG
BbsI 656 GTCTTC GAAGAC(2/6)
BcgI 413 CGATCCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1075 CTAG C^TAG
BglII 707 AGATCT A^GATCT
BpuAI 656 GTCTTC GAAGAC(2/6)
BsaAI 733 CACGTG [CT]AC^GT[GA]
BsiEI 126 CGACCG CG[GA][CT]^CG
BsiHKAI 421 GTGCAC G[AT]GC[AT]^C
BsmBI 996 CGTCTC CGTCTC(1/5)
BsmI 789 GAATGC GAATGC(1/-1)
Bsp1407I 305 TGTACA T^GTACA
BspHI 861 TCATGA T^CATGA
BspMI 1028 ACCTGC ACCTGC(4/8)
BsrDI 1013 GCAATG GCAATG(2/0)
BsrGI 305 TGTACA T^GTACA
BsrI 1168 CCAGT ACTGG(1/-1)
BstAPI 423 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 24 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 252 CCTGAGG CC^T[ACGT]AGG
EarI 1003 CTCTTC CTCTTC(1/4)
Eco81I 252 CCTGAGG CC^T[ACGT]AGG
FseI 3 GGCCGGCC GGCCGG^CC
HaeII 821 AGCGCC [GA]GCGC^[CT]
HphI 992 TCACC GGTGA(8/7)
Ksp632I 1003 CTCTTC CTCTTC(1/4)
MaeI 1075 CTAG C^TAG
MaeII 734 ACGT A^CGT
MaeIII 991 GTCAC ^GT[ACGT]AC
MluNI 187 TGGCCA TGG^CCA
MscI 187 TGGCCA TGG^CCA
MslI 630 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4 GCCGGC GCC^GGC
NdeI 923 CATATG CA^TATG
NgoMIV 4 GCCGGC G^CCGGC
PmaCI 733 CACGTG CAC^GTG
PmlI 733 CACGTG CAC^GTG
PpuMI 87 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 861 TCATGA T^CATGA
SfcI 1193 CTGTAG C^T[GA][CT]AG
SmlI 838 CTCAAG C^T[CT][GA]AG
SspBI 305 TGTACA T^GTACA
TfiI 966 GAATC G^A[AT]TC
Tsp45I 991 GTCAC ^GT[GC]AC
XcmI 661 CCACTACCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 282 TCCGGA T^CCGGA
950 TCCGGA
AlwNI 229 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
820 CAGCGCCTG
AviII 467 TGCGCA TGC^GCA
605 TGCGCA
BbvCI 439 GCTGAGG CCTCAGC(-5/-2)
453 GCTGAGG
BglI 133 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
505 GCCACCGAGGC
BsaI 1242 GAGACC GGTCTC(1/5)
1125 GGTCTC
BsaWI 282 TCCGGA [AT]^CCGG[AT]
950 TCCGGA
BseAI 282 TCCGGA T^CCGGA
950 TCCGGA
BseRI 255 GAGGAG GAGGAG(10/8)
298 GAGGAG
BsmFI 579 GGGAC GGGAC(10/14)
476 GTCCC
BspEI 282 TCCGGA T^CCGGA
950 TCCGGA
BsrFI 4 GCCGGC [GA]^CCGG[CT]
290 ACCGGC
BssSI 191 CACGAG CACGAG(-5/-1)
487 CACGAG
BstF5I 309 CATCC GGATG(2/0)
1155 CATCC
BstYI 707 AGATCT [GA]^GATC[CT]
779 GGATCC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
290 ACCGGC
DraIII 180 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
424 CACACGGTG
FokI 309 CATCC GGATG(9/13)
1155 CATCC
FspI 467 TGCGCA TGC^GCA
605 TGCGCA
MboII 657 TCTTC GAAGA(8/7)
1004 TCTTC
MflI 707 AGATCT [GA]^GATC[CT]
779 GGATCC
MroI 282 TCCGGA T^CCGGA
950 TCCGGA
SfiI 132 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
504 GGCCACCGAGGCC
StuI 195 AGGCCT AGG^CCT
269 AGGCCT
TspRI 602 CACTG CA[GC]TG[ACGT][ACGT]^
1169 CAGTG
XhoII 707 AGATCT [GA]^GATC[CT]
779 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsoBI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SmaI
StyI TseI Van91I XmaCI XmaI