Restriction map for
pFN21AB7205
(Linear) (Six-base) MAPSORT of: KIBB7205.seq Check: 9045 from: 1 to: 1278
KIBB7205.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1037 1278
Size: 1037 241
AlwNI CAG_nnn'CTG
Cuts at: 0 234 825 1278
Size: 234 591 453
Fragments arranged by size:
591 453 234
ApaI G_GGCC'C
Cuts at: 0 78 135 742 894 1273 1278
Size: 78 57 607 152 379 5
Fragments arranged by size:
607 379 152 78 57 5
ApaLI G'TGCA_C
Cuts at: 0 421 1278
Size: 421 857
AvaI C'yCGr_G
Cuts at: 0 71 373 394 746 812 1051 1278
Size: 71 302 21 352 66 239 227
Fragments arranged by size:
352 302 239 227 71 66 21
BamHI G'GATC_C
Cuts at: 0 779 1278
Size: 779 499
BanI G'GyrC_C
Cuts at: 0 429 1055 1173 1250 1278
Size: 429 626 118 77 28
Fragments arranged by size:
626 429 118 77 28
BanII G_rGCy'C
Cuts at: 0 78 135 394 732 742 894 1273 1278
Size: 78 57 259 338 10 152 379 5
Fragments arranged by size:
379 338 259 152 78 57 10 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 649 1278
Size: 649 629
BbvCI CC'TCA_GC
Cuts at: 0 440 454 1278
Size: 440 14 824
Fragments arranged by size:
824 440 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 402 436 1278
Size: 402 34 842
Fragments arranged by size:
842 402 34
BglI GCCn_nnn'nGGC
Cuts at: 0 139 511 1278
Size: 139 372 767
Fragments arranged by size:
767 372 139
BglII A'GATC_T
Cuts at: 0 707 1278
Size: 707 571
Bme1580I G_kGCm'C
Cuts at: 0 78 135 348 425 434 742 894 1060
Size: 78 57 213 77 9 308 152 166
Cuts at: 1060 1178 1273 1278
Size: 118 95 5
Fragments arranged by size:
308 213 166 152 118 95 78 77
57 9 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1278
Size: 192 1086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 358 368 426 556 586 868 1151
Size: 256 102 10 58 130 30 282 283
Cuts at: 1151 1260 1278
Size: 109 18
Fragments arranged by size:
283 282 256 130 109 102 58 30
18 10
Bpu10I CC'TnA_GC
Cuts at: 0 440 454 1278
Size: 440 14 824
Fragments arranged by size:
824 440 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1278
Size: 823 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 1131 1236 1278
Size: 1131 105 42
Fragments arranged by size:
1131 105 42
BsaAI yAC'GTr
Cuts at: 0 735 1278
Size: 735 543
BsaWI w'CCGG_w
Cuts at: 0 282 950 1278
Size: 282 668 328
Fragments arranged by size:
668 328 282
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 719 749 974 1004 1278
Size: 289 30 400 30 225 30 274
Fragments arranged by size:
400 289 274 225 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 313 1278
Size: 270 43 965
Fragments arranged by size:
965 270 43
BseYI C'CCAG_C
Cuts at: 0 240 764 1161 1224 1278
Size: 240 524 397 63 54
Fragments arranged by size:
524 397 240 63 54
BsiEI CG_ry'CG
Cuts at: 0 129 1278
Size: 129 1149
BsiHKAI G_wGCw'C
Cuts at: 0 425 1278
Size: 425 853
BsmI GAATG_Cn'
Cuts at: 0 795 1278
Size: 795 483
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1002 1278
Size: 1002 276
Bsp1286I G_dGCh'C
Cuts at: 0 78 135 348 394 425 434 732 742
Size: 78 57 213 46 31 9 298 10
Cuts at: 742 894 1060 1178 1273 1278
Size: 152 166 118 95 5
Fragments arranged by size:
298 213 166 152 118 95 78 57
46 31 10 9 5
BspEI T'CCGG_A
Cuts at: 0 282 950 1278
Size: 282 668 328
Fragments arranged by size:
668 328 282
BspHI T'CATG_A
Cuts at: 0 861 1278
Size: 861 417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1037 1278
Size: 1037 241
BsrDI GCAATG_nn'
Cuts at: 0 1020 1278
Size: 1020 258
BsrFI r'CCGG_y
Cuts at: 0 4 290 1278
Size: 4 286 988
Fragments arranged by size:
988 286 4
BsrGI T'GTAC_A
Cuts at: 0 305 1278
Size: 305 973
BssSI C'ACGA_G
Cuts at: 0 191 487 1278
Size: 191 296 791
Fragments arranged by size:
791 296 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 1278
Size: 429 849
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 1278
Size: 31 1247
BstYI r'GATC_y
Cuts at: 0 707 779 1278
Size: 707 72 499
Fragments arranged by size:
707 499 72
Bsu36I CC'TnA_GG
Cuts at: 0 253 1278
Size: 253 1025
BtgI C'CryG_G
Cuts at: 0 120 152 212 434 651 1119 1134 1278
Size: 120 32 60 222 217 468 15 144
Fragments arranged by size:
468 222 217 144 120 60 32 15
BtsI GCAGTG_nn'
Cuts at: 0 601 1278
Size: 601 677
DraIII CAC_nnn'GTG
Cuts at: 0 185 429 1278
Size: 185 244 849
Fragments arranged by size:
849 244 185
EaeI y'GGCC_r
Cuts at: 0 2 187 1184 1278
Size: 2 185 997 94
Fragments arranged by size:
997 185 94 2
EarI CTCTTCn'nnn_
Cuts at: 0 1009 1278
Size: 1009 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 214 1011 1278
Size: 106 108 797 267
Fragments arranged by size:
797 267 108 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 358 368 426 556 586 868 1151
Size: 256 102 10 58 130 30 282 283
Cuts at: 1151 1260 1278
Size: 109 18
Fragments arranged by size:
283 282 256 130 109 102 58 30
18 10
EcoO109I rG'GnC_Cy
Cuts at: 0 75 88 132 531 890 891 1269 1270
Size: 75 13 44 399 359 1 378 1
Cuts at: 1270 1278
Size: 8
Fragments arranged by size:
399 378 359 75 44 13 8 1
1
FseI GG_CCGG'CC
Cuts at: 0 8 1278
Size: 8 1270
FspI TGC'GCA
Cuts at: 0 469 607 1278
Size: 469 138 671
Fragments arranged by size:
671 469 138
HaeII r_GCGC'y
Cuts at: 0 825 1278
Size: 825 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 974 1006 1278
Size: 974 32 272
Fragments arranged by size:
974 272 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1278
Size: 573 705
MscI TGG'CCA
Cuts at: 0 189 1278
Size: 189 1089
MslI CAynn'nnrTG
Cuts at: 0 634 1278
Size: 634 644
MspA1I CmG'CkG
Cuts at: 0 138 207 234 404 473 768 1092 1278
Size: 138 69 27 170 69 295 324 186
Fragments arranged by size:
324 295 186 170 138 69 69 27
NaeI GCC'GGC
Cuts at: 0 6 1278
Size: 6 1272
NcoI C'CATG_G
Cuts at: 0 120 152 1119 1134 1278
Size: 120 32 967 15 144
Fragments arranged by size:
967 144 120 32 15
NdeI CA'TA_TG
Cuts at: 0 924 1278
Size: 924 354
NgoMIV G'CCGG_C
Cuts at: 0 4 1278
Size: 4 1274
PasI CC'CwG_GG
Cuts at: 0 894 1278
Size: 894 384
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 234 825 1278
Size: 185 49 591 453
Fragments arranged by size:
591 453 185 49
PmlI CAC'GTG
Cuts at: 0 735 1278
Size: 735 543
PpuMI rG'GwC_Cy
Cuts at: 0 88 1278
Size: 88 1190
PspOMI G'GGCC_C
Cuts at: 0 74 131 738 890 1269 1278
Size: 74 57 607 152 379 9
Fragments arranged by size:
607 379 152 74 57 9
PvuII CAG'CTG
Cuts at: 0 207 234 473 768 1092 1278
Size: 207 27 239 295 324 186
Fragments arranged by size:
324 295 239 207 186 27
SfcI C'TryA_G
Cuts at: 0 1193 1278
Size: 1193 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 139 511 1278
Size: 139 372 767
Fragments arranged by size:
767 372 139
SmaI CCC'GGG
Cuts at: 0 73 748 814 1053 1278
Size: 73 675 66 239 225
Fragments arranged by size:
675 239 225 73 66
SmlI C'TyrA_G
Cuts at: 0 838 1278
Size: 838 440
StuI AGG'CCT
Cuts at: 0 197 271 1278
Size: 197 74 1007
Fragments arranged by size:
1007 197 74
StyI C'CwwG_G
Cuts at: 0 120 152 1119 1134 1262 1278
Size: 120 32 967 15 128 16
Fragments arranged by size:
967 128 120 32 16 15
TatI w'GTAC_w
Cuts at: 0 305 851 1278
Size: 305 546 427
Fragments arranged by size:
546 427 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 719 751 1278
Size: 80 32 607 32 527
Fragments arranged by size:
607 527 80 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 668 1278
Size: 668 610
XmaI C'CCGG_G
Cuts at: 0 71 746 812 1051 1278
Size: 71 675 66 239 227
Fragments arranged by size:
675 239 227 71 66
Enzymes that do cut:
AarI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI BglII Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
FseI FspI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV PasI PflMI PmlI PpuMI
PspOMI PvuII SfcI SfiI SmaI SmlI StuI StyI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BsaBI BsaHI BsgI BsiWI BsrBI
BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI