(Circular) (Six-base) MAPSORT of: pFN21AB7202.seq Check: 8098 from: 1 to: 5428 pFN21AB7202 5428 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3491 4875 5143 1632 Size: 1859 1384 268 1917 Fragments arranged by size: 1917 1859 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 2434 3171 4743 2434 Size: 737 1572 3119 Fragments arranged by size: 3119 1572 737 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 5428 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2737 2963 3115 5224 1493 Size: 1244 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2311 2399 2753 1870 Size: 73 98 270 88 354 4545 Fragments arranged by size: 4545 354 270 98 88 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2257 2290 2257 Size: 33 5395 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2143 2505 2749 4204 618 Size: 325 217 627 356 362 244 1455 1842 Fragments arranged by size: 1842 1455 627 362 356 325 244 217 BanII G_rGCy'C Cuts at: 1391 2050 2292 2568 2595 2747 1391 Size: 659 242 276 27 152 4072 Fragments arranged by size: 4072 659 276 242 152 27 BbeI G_GCGC'C Cuts at: 2509 2509 Size: 5428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4457 BbvCI CC'TCA_GC Cuts at: 2660 2660 Size: 5428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2507 2541 3619 3653 1903 Size: 34 570 34 1078 34 3678 Fragments arranged by size: 3678 1078 570 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2285 2433 3320 4530 2285 Size: 148 887 1210 3183 Fragments arranged by size: 3183 1210 887 148 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2572 2674 4039 Size: 107 122 71 631 605 900 102 1365 Cuts at: 4039 136 Size: 1525 Fragments arranged by size: 1525 1365 900 631 605 122 107 102 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2050 2146 2318 3489 4645 1790 Size: 260 96 172 1171 1156 2573 Fragments arranged by size: 2573 1171 1156 260 172 96 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2480 3159 4117 Size: 416 53 83 399 24 1063 679 958 Cuts at: 4117 442 Size: 1753 Fragments arranged by size: 1753 1063 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2306 4088 1239 Size: 980 87 1782 2579 Fragments arranged by size: 2579 1782 980 87 Bpu10I CC'TnA_GC Cuts at: 2660 2660 Size: 5428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2317 3553 4418 4716 2317 Size: 1236 865 298 3029 Fragments arranged by size: 3029 1236 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4091 882 Size: 617 2592 2219 Fragments arranged by size: 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 3219 493 Size: 711 1164 851 2702 Fragments arranged by size: 2702 1164 851 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 2506 3235 3617 Size: 53 83 186 936 649 324 729 382 Cuts at: 3617 4252 275 Size: 635 1451 Fragments arranged by size: 1451 936 729 649 635 382 324 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2212 2242 1178 Size: 30 165 30 809 30 4364 Fragments arranged by size: 4364 809 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2282 1186 Size: 941 155 4332 Fragments arranged by size: 4332 941 155 BseYI C'CCAG_C Cuts at: 2133 2147 2371 2427 2446 2453 4631 5127 2133 Size: 14 224 56 19 7 2178 496 2434 Fragments arranged by size: 2434 2178 496 224 56 19 14 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 1163 Size: 573 747 4108 Fragments arranged by size: 4108 747 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2080 2189 2251 2878 3639 4667 Size: 275 186 79 109 62 627 761 1028 Cuts at: 4667 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 627 275 186 109 79 62 BsiHKAI G_wGCw'C Cuts at: 2292 2466 3489 3574 4645 2292 Size: 174 1023 85 1071 3075 Fragments arranged by size: 3075 1071 1023 174 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2146 2292 2318 2466 2568 2595 Size: 399 260 96 146 26 148 102 27 Cuts at: 2595 2747 3489 3574 4645 1391 Size: 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 260 152 148 146 102 96 85 27 26 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2783 844 Size: 1939 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2211 3315 1394 Size: 429 388 1104 3507 Fragments arranged by size: 3507 1104 429 388 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2673 4072 4259 1700 Size: 110 863 1399 187 2869 Fragments arranged by size: 2869 1399 863 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 2339 3488 4500 2339 Size: 1149 1012 3267 Fragments arranged by size: 3267 1149 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2641 2758 3125 3510 3527 4979 5065 5077 Size: 669 117 367 385 17 1452 86 12 Cuts at: 5077 5423 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 669 385 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2410 513 Size: 552 939 406 3531 Fragments arranged by size: 3531 939 552 406 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2248 2875 Size: 54 1425 50 275 18 247 171 627 Cuts at: 2875 3764 5393 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 275 247 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2077 2248 2875 1537 Size: 275 265 171 627 4090 Fragments arranged by size: 4090 627 275 265 171 EarI CTCTTCn'nnn_ Cuts at: 1353 2209 2583 3358 1353 Size: 856 374 775 3423 Fragments arranged by size: 3423 856 775 374 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4015 4389 4535 387 Size: 1064 138 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2290 2290 Size: 5428 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2306 3491 4088 4875 5143 1239 Size: 393 587 87 1185 597 787 268 1524 Fragments arranged by size: 1524 1185 787 597 587 393 268 87 EcoNI CCTnn'n_nnAGG Cuts at: 2615 2615 Size: 5428 EcoO109I rG'GnC_Cy Cuts at: 2046 2167 2214 2382 2395 2608 2667 2046 Size: 121 47 168 13 213 59 4807 Fragments arranged by size: 4807 213 168 121 59 47 13 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 2454 2509 4575 1678 Size: 776 55 2066 2531 Fragments arranged by size: 2531 2066 776 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373 Size: 32 135 32 2497 32 42 32 2626 Fragments arranged by size: 2626 2497 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KasI G'GCGC_C Cuts at: 2505 2505 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4542 4726 1305 Size: 3237 184 2007 Fragments arranged by size: 3237 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3386 3745 3904 518 Size: 648 843 1377 359 159 2042 Fragments arranged by size: 2042 1377 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2032 2133 2443 2687 2844 3521 4669 Size: 417 9 101 310 244 157 677 1148 Cuts at: 4669 4914 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 417 310 245 244 157 101 9 NaeI GCC'GGC Cuts at: 2675 2675 Size: 5428 NarI GG'CG_CC Cuts at: 2506 2506 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 2004 2410 513 Size: 552 939 406 3531 Fragments arranged by size: 3531 939 552 406 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NgoMIV G'CCGG_C Cuts at: 2673 2673 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2786 4331 1132 Size: 97 1557 1545 2229 Fragments arranged by size: 2229 1557 1545 97 PasI CC'CwG_GG Cuts at: 1348 2060 2084 1348 Size: 712 24 4692 Fragments arranged by size: 4692 712 24 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 1108 Size: 1025 4403 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PmlI CAC'GTG Cuts at: 2368 2368 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2214 2382 2608 2214 Size: 168 226 5034 Fragments arranged by size: 5034 226 168 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 5428 PstI C_TGCA'G Cuts at: 830 2022 2780 830 Size: 1192 758 3478 Fragments arranged by size: 3478 1192 758 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 PvuII CAG'CTG Cuts at: 2023 2023 Size: 5428 SacI G_AGCT'C Cuts at: 2292 2292 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SanDI GG'GwC_CC Cuts at: 2214 2214 Size: 5428 SapI GCTCTTCn'nnn_ Cuts at: 2583 2583 Size: 5428 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2018 2776 3911 4592 4783 826 Size: 219 973 758 1135 681 191 1471 Fragments arranged by size: 1471 1135 973 758 681 219 191 SfoI GGC'GCC Cuts at: 2507 2507 Size: 5428 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2401 2755 1872 Size: 529 354 4545 Fragments arranged by size: 4545 529 354 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 2296 3532 4433 4695 820 Size: 197 926 98 255 1236 901 262 1553 Fragments arranged by size: 1553 1236 926 901 262 255 197 98 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2004 2050 2410 2867 513 Size: 552 939 46 360 457 3074 Fragments arranged by size: 3074 939 552 457 360 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2161 2203 3455 3472 3625 3810 4255 Size: 1255 581 42 1252 17 153 185 445 Cuts at: 4255 325 Size: 1498 Fragments arranged by size: 1498 1255 1252 581 445 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3674 5386 96 Size: 274 80 33 51 1443 1697 1712 138 Fragments arranged by size: 1712 1697 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2136 2168 2212 2244 5388 5420 2136 Size: 32 44 32 3144 32 2144 Fragments arranged by size: 3144 2144 44 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2057 2057 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 2041 1943 Size: 98 5330 XmaI C'CCGG_G Cuts at: 1870 2399 2753 1870 Size: 529 354 4545 Fragments arranged by size: 4545 529 354 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI