Restriction map for
pFN21AB7199
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbsI BbvCI BciVI
BclI BfrI BlnI BpuAI BsaBI
BsaI BseAI BsgI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 1081 GAATTT [GA]^AATT[CT]
AcyI 709 GGCGCC G[GA]^CG[CT]C
AflIII 840 ACATGT A^C[GA][CT]GT
Alw44I 961 GTGCAC G^TGCAC
AlwNI 745 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 961 GTGCAC G^TGCAC
ApoI 1081 GAATTT [GA]^AATT[CT]
AscI 58 GGCGCGCC GG^CGCGCC
AviII 1208 TGCGCA TGC^GCA
BalI 605 TGGCCA TGG^CCA
BbeI 709 GGCGCC GGCGC^C
BbrPI 532 CACGTG CAC^GTG
BglII 1010 AGATCT A^GATCT
BpmI 446 CTCCAG CTGGAG(16/14)
BsaAI 532 CACGTG [CT]AC^GT[GA]
BsaHI 709 GGCGCC G[GA]^CG[CT]C
BsaWI 806 ACCGGA [AT]^CCGG[AT]
BseRI 898 GAGGAG GAGGAG(10/8)
BsmFI 122 GGGAC GGGAC(10/14)
BspHI 1227 TCATGA T^CATGA
BspLU11I 840 ACATGT A^CATGT
BssHII 59 GCGCGC G^CGCGC
BssSI 73 CACGAG CACGAG(-5/-1)
BstAPI 1169 GCAAAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT14I 848 CCATGG C^C[AT][AT]GG
FspI 1208 TGCGCA TGC^GCA
HgaI 1100 GACGC GACGC(5/10)
Hin1I 709 GGCGCC G[GA]^CG[CT]C
HincII 946 GTCAAC GT[CT]^[GA]AC
HindII 946 GTCAAC GT[CT]^[GA]AC
KasI 709 GGCGCC G^GCGCC
KspI 182 CCGCGG CCGC^GG
MluNI 605 TGGCCA TGG^CCA
MscI 605 TGGCCA TGG^CCA
MseI 1385 TTAA T^TAA
NarI 709 GGCGCC GG^CGCC
NcoI 848 CCATGG C^CATGG
PleI 124 GACTC GAGTC(4/5)
PmaCI 532 CACGTG CAC^GTG
PmlI 532 CACGTG CAC^GTG
PpuMI 1048 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 837 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1337 CTGCAG CTGCA^G
RcaI 1227 TCATGA T^CATGA
SacII 182 CCGCGG CCGC^GG
SexAI 545 ACCAGGT A^CC[AT]GGT
SfoI 709 GGCGCC GGC^GCC
SphI 441 GCATGC GCATG^C
StyI 848 CCATGG C^C[AT][AT]GG
TaqI 767 TCGA T^CGA
Tru9I 1385 TTAA T^TAA
TthHB8I 767 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 936 GTAC GT^AC
1395 GTAC
AlwI 1038 GATCC GGATC(4/5)
764 GGATC
ApaI 703 GGGCCC GGGCC^C
864 GGGCCC
BfaI 470 CTAG C^TAG
1349 CTAG
BglI 421 GCCGGCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
522 GCCCACAGGGC
BstF5I 456 CATCC GGATG(2/0)
954 CATCC
BstXI 245 CCAACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1359 CCACAACCCTGG
BstYI 1010 AGATCT [GA]^GATC[CT]
1037 AGATCC
DraII 863 GGGGCCC [GA]G^G[ACGT]CC[CT]
1048 AGGACCC
EagI 579 CGGCCG C^GGCCG
632 CGGCCG
EarI 415 CTCTTC CTCTTC(1/4)
795 GAAGAG
EclXI 579 CGGCCG C^GGCCG
632 CGGCCG
Eco52I 579 CGGCCG C^GGCCG
632 CGGCCG
EcoO109I 863 GGGGCCC [GA]G^G[ACGT]CC[CT]
1048 AGGACCC
EcoT22I 823 ATGCAT ATGCA^T
1135 ATGCAT
FokI 456 CATCC GGATG(9/13)
954 CATCC
Ksp632I 415 CTCTTC CTCTTC(1/4)
795 GAAGAG
MaeI 470 CTAG C^TAG
1349 CTAG
MflI 1010 AGATCT [GA]^GATC[CT]
1037 AGATCC
NsiI 823 ATGCAT ATGCA^T
1135 ATGCAT
PvuII 449 CAGCTG CAG^CTG
778 CAGCTG
RsaI 936 GTAC GT^AC
1395 GTAC
SacI 210 GAGCTC GAGCT^C
1200 GAGCTC
SapI 795 GAAGAGC GCTCTTC(1/4)
414 GCTCTTC
SmlI 1033 CTCAAG C^T[CT][GA]AG
1235 CTCAAG
Tsp509I 1082 AATT ^AATT
1381 AATT
TspRI 381 CAGTG CA[GC]TG[ACGT][ACGT]^
602 CAGTG
XhoII 1010 AGATCT [GA]^GATC[CT]
1037 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AluI Asp700I AspHI
AvaI AvaII BanI BanII BbvI
BcgI BcnI BlpI BmyI Bpu1102I
BsaJI BsiEI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI TfiI TseI Tsp45I
XcmI XmaCI XmaI XmnI