(Circular) (Six-base) MAPSORT of: pFN21AB7199.seq Check: 7396 from: 1 to: 6106 pFN21AB7199 6106 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 2803 4169 5553 5821 1632 Size: 558 613 1366 1384 268 1917 Fragments arranged by size: 1917 1384 1366 613 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 2845 5005 5982 1203 Size: 1642 2160 977 1327 Fragments arranged by size: 2160 1642 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 3063 1166 Size: 1897 4209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 2755 3849 5421 2755 Size: 1094 1572 3440 Fragments arranged by size: 3440 1572 1094 ApaI G_GGCC'C Cuts at: 2712 2873 2712 Size: 161 5945 ApaLI G'TGCA_C Cuts at: 2966 4163 5319 2966 Size: 1197 1156 3753 Fragments arranged by size: 3753 1197 1156 ApoI r'AATT_y Cuts at: 1493 3086 3415 3641 3793 5902 1493 Size: 1593 329 226 152 2109 1697 Fragments arranged by size: 2109 1697 1593 329 226 152 AscI GG'CGCG_CC Cuts at: 2064 2064 Size: 6106 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2398 2465 2492 2913 3001 3202 3431 Size: 73 455 67 27 421 88 201 229 Cuts at: 3431 1870 Size: 4545 Fragments arranged by size: 4545 455 421 229 201 88 73 67 27 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2517 2714 3427 4882 Size: 325 217 627 518 212 197 713 1455 Cuts at: 4882 618 Size: 1842 Fragments arranged by size: 1842 1455 713 627 518 325 217 212 197 BanII G_rGCy'C Cuts at: 1391 2183 2219 2385 2421 2472 2712 2873 3209 Size: 792 36 166 36 51 240 161 336 Cuts at: 3209 3425 1391 Size: 216 4072 Fragments arranged by size: 4072 792 336 240 216 166 161 51 36 36 BbeI G_GCGC'C Cuts at: 2718 2718 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2425 2459 2752 2786 3193 3227 4297 Size: 34 488 34 293 34 407 34 1070 Cuts at: 4297 4331 1903 Size: 34 3678 Fragments arranged by size: 3678 1070 488 407 293 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BfrBI ATG'CAT Cuts at: 2830 3142 2830 Size: 312 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 2533 4717 Size: 107 122 71 631 605 760 101 2184 Cuts at: 4717 136 Size: 1525 Fragments arranged by size: 2184 1525 760 631 605 122 107 101 71 BglII A'GATC_T Cuts at: 3015 6101 3015 Size: 3086 3020 BlpI GC'TnA_GC Cuts at: 2177 2781 2786 3523 2177 Size: 604 5 737 4760 Fragments arranged by size: 4760 737 604 5 Bme1580I G_kGCm'C Cuts at: 1790 2310 2520 2538 2712 2873 2912 2970 4167 Size: 520 210 18 174 161 39 58 1197 Cuts at: 4167 5323 1790 Size: 1156 2573 Fragments arranged by size: 2573 1197 1156 520 210 174 161 58 39 18 BmgBI CAC'GTC Cuts at: 1318 2890 1318 Size: 1572 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2600 2908 3837 Size: 416 53 83 399 24 1183 308 929 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1183 958 929 416 399 308 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2413 2413 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2436 4766 1239 Size: 1197 2330 2579 Fragments arranged by size: 2579 2330 1197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 3225 4231 5096 5394 3023 Size: 202 1006 865 298 3735 Fragments arranged by size: 3735 1006 865 298 202 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2539 3897 493 Size: 711 1335 1358 2702 Fragments arranged by size: 2702 1358 1335 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2715 3913 4295 4930 Size: 53 83 186 936 1182 1198 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1451 1198 1182 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2811 4539 4937 5211 5358 5769 2811 Size: 1728 398 274 147 411 3148 Fragments arranged by size: 3148 1728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2676 2706 2744 2774 2909 Size: 30 165 30 1273 30 38 30 135 Cuts at: 2909 2939 3191 3221 1178 Size: 30 252 30 4063 Fragments arranged by size: 4063 1273 252 165 135 38 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2918 1186 Size: 1732 4374 BseYI C'CCAG_C Cuts at: 2044 2309 2513 5309 5805 2044 Size: 265 204 2796 496 2345 Fragments arranged by size: 2796 2345 496 265 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 2640 2812 3127 3556 4317 Size: 275 186 586 53 172 315 429 761 Cuts at: 4317 5345 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 586 429 315 275 186 172 53 BsiHKAI G_wGCw'C Cuts at: 2219 2970 3209 4167 4252 5323 2219 Size: 751 239 958 85 1071 3002 Fragments arranged by size: 3002 1071 958 751 239 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2219 2310 2385 2421 2472 2520 Size: 399 393 36 91 75 36 51 48 Cuts at: 2520 2538 2712 2873 2912 2970 3209 3425 4167 Size: 18 174 161 39 58 239 216 742 Cuts at: 4167 4252 5323 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 239 216 174 161 91 85 75 58 51 48 39 36 36 18 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 3232 3995 5725 3232 Size: 763 1730 3613 Fragments arranged by size: 3613 1730 763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 844 Size: 2617 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2426 2639 2790 4750 4937 1700 Size: 110 288 328 213 151 1960 187 2869 Fragments arranged by size: 2869 1960 328 288 213 187 151 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 2064 1925 Size: 139 5967 BssSI C'ACGA_G Cuts at: 2078 4166 5178 2078 Size: 2088 1012 3006 Fragments arranged by size: 3006 2088 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3180 794 Size: 2386 3720 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 2257 3371 2257 Size: 1114 4992 BstYI r'GATC_y Cuts at: 1972 3015 3042 3436 3803 4188 4205 5657 5743 Size: 1043 27 394 367 385 17 1452 86 Cuts at: 5743 5755 6101 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1043 394 385 367 346 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2187 2704 2853 513 Size: 552 1122 517 149 3766 Fragments arranged by size: 3766 1122 552 517 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 3622 4416 4436 3622 Size: 794 20 5292 Fragments arranged by size: 5292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2584 2610 2637 Size: 54 1425 50 275 18 754 26 27 Cuts at: 2637 3553 4442 6071 8 Size: 916 889 1629 43 Fragments arranged by size: 1629 1425 916 889 754 275 54 50 43 27 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2584 2637 3553 1537 Size: 275 772 53 916 4090 Fragments arranged by size: 4090 916 772 275 53 EarI CTCTTCn'nnn_ Cuts at: 1353 2426 2795 4036 1353 Size: 1073 369 1241 3423 Fragments arranged by size: 3423 1241 1073 369 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 4693 5067 5213 387 Size: 1064 138 765 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 765 374 146 138 EcoICRI GAG'CTC Cuts at: 2217 3207 2217 Size: 990 5116 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2436 2803 4169 4766 5553 5821 Size: 393 558 246 367 1366 597 787 268 Cuts at: 5821 1239 Size: 1524 Fragments arranged by size: 1524 1366 787 597 558 393 367 268 246 EcoO109I rG'GnC_Cy Cuts at: 2869 3054 2869 Size: 185 5921 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 3215 4612 3215 Size: 1397 4709 HaeII r_GCGC'y Cuts at: 1678 2325 2514 2646 2718 5253 1678 Size: 647 189 132 72 2535 2531 Fragments arranged by size: 2535 2531 647 189 132 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2941 4747 4779 4821 Size: 32 135 32 1337 32 1806 32 42 Cuts at: 4821 4853 1373 Size: 32 2626 Fragments arranged by size: 2626 1806 1337 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2953 3450 3696 669 Size: 988 281 1015 497 246 3079 Fragments arranged by size: 3079 1015 988 497 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2714 2714 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 10 Size: 54 1425 343 780 3504 Fragments arranged by size: 3504 1425 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2672 2678 2684 2963 3063 3141 Size: 648 843 663 6 6 279 100 78 Cuts at: 3141 3143 4064 4423 4582 518 Size: 2 921 359 159 2042 Fragments arranged by size: 2042 921 843 663 648 359 279 159 100 78 6 6 2 MspA1I CmG'CkG Cuts at: 1606 2165 2189 2456 2785 3522 4199 5347 5592 Size: 559 24 267 329 737 677 1148 245 Cuts at: 5592 1606 Size: 2120 Fragments arranged by size: 2120 1148 737 677 559 329 267 245 24 NaeI GCC'GGC Cuts at: 2100 2428 2641 2792 2100 Size: 328 213 151 5414 Fragments arranged by size: 5414 328 213 151 NarI GG'CG_CC Cuts at: 2715 2715 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 2853 513 Size: 552 1788 3766 Fragments arranged by size: 3766 1788 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NgoMIV G'CCGG_C Cuts at: 2098 2426 2639 2790 2098 Size: 328 213 151 5414 Fragments arranged by size: 5414 328 213 151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NsiI A_TGCA'T Cuts at: 2832 3144 2832 Size: 312 5794 NspI r_CATG'y Cuts at: 1132 1229 2450 2849 2864 3142 3146 3464 5009 Size: 97 1221 399 15 278 4 318 1545 Cuts at: 5009 1132 Size: 2229 Fragments arranged by size: 2229 1545 1221 399 318 278 97 15 4 PasI CC'CwG_GG Cuts at: 1348 2039 2257 1348 Size: 691 218 5197 Fragments arranged by size: 5197 691 218 PciI A'CATG_T Cuts at: 2845 5005 2845 Size: 2160 3946 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PfoI T'CCnGG_A Cuts at: 2016 2016 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PmlI CAC'GTG Cuts at: 2539 2539 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2377 4175 4207 5688 5720 2345 Size: 32 1798 32 1481 32 2731 Fragments arranged by size: 2731 1798 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3054 3054 Size: 6106 PshAI GACnn'nnGTC Cuts at: 1613 2846 1613 Size: 1233 4873 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 2708 2869 2708 Size: 161 5945 PstI C_TGCA'G Cuts at: 830 3346 3458 830 Size: 2516 112 3478 Fragments arranged by size: 3478 2516 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2456 2785 2456 Size: 329 5777 SacI G_AGCT'C Cuts at: 2219 3209 2219 Size: 990 5116 SacII CC_GC'GG Cuts at: 2190 2190 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 2426 2795 2426 Size: 369 5737 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SexAI A'CCwGG_T Cuts at: 2550 2550 Size: 6106 SfcI C'TryA_G Cuts at: 826 1045 2659 3300 3342 3454 4589 5270 5461 Size: 219 1614 641 42 112 1135 681 191 Cuts at: 5461 826 Size: 1471 Fragments arranged by size: 1614 1471 1135 681 641 219 191 112 42 SfoI GGC'GCC Cuts at: 2716 2716 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 2494 2915 3003 3433 1872 Size: 622 421 88 430 4545 Fragments arranged by size: 4545 622 430 421 88 SmlI C'TyrA_G Cuts at: 820 1017 1943 3038 3240 4210 5111 5373 820 Size: 197 926 1095 202 970 901 262 1553 Fragments arranged by size: 1553 1095 970 926 901 262 202 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2450 3464 1132 Size: 1318 1014 3774 Fragments arranged by size: 3774 1318 1014 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2853 3545 513 Size: 552 1788 692 3074 Fragments arranged by size: 3074 1788 692 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2940 4352 6064 Size: 274 80 33 51 1443 963 1412 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1712 1443 1412 963 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 2744 2776 3191 3223 6066 6098 2676 Size: 32 36 32 415 32 2843 32 2684 Fragments arranged by size: 2843 2684 415 36 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2536 2833 2860 2536 Size: 297 27 5782 Fragments arranged by size: 5782 297 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 2492 2913 3001 3431 1870 Size: 622 421 88 430 4545 Fragments arranged by size: 4545 622 430 421 88 XmnI GAAnn'nnTTC Cuts at: 2120 2984 3074 4235 2120 Size: 864 90 1161 3991 Fragments arranged by size: 3991 1161 864 90 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI