Restriction map for
pFN21AB7199
(Linear) (Six-base) MAPSORT of: KIBB7199.seq Check: 3592 from: 1 to: 1401
KIBB7199.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 798 1401
Size: 185 613 603
Fragments arranged by size:
613 603 185
AflIII A'CryG_T
Cuts at: 0 840 1401
Size: 840 561
AleI CACnn'nnGTG
Cuts at: 0 1058 1401
Size: 1058 343
AlwNI CAG_nnn'CTG
Cuts at: 0 750 1401
Size: 750 651
ApaI G_GGCC'C
Cuts at: 0 707 868 1401
Size: 707 161 533
Fragments arranged by size:
707 533 161
ApaLI G'TGCA_C
Cuts at: 0 961 1401
Size: 961 440
ApoI r'AATT_y
Cuts at: 0 1081 1401
Size: 1081 320
AscI GG'CGCG_CC
Cuts at: 0 59 1401
Size: 59 1342
AvaI C'yCGr_G
Cuts at: 0 393 460 487 908 996 1197 1401
Size: 393 67 27 421 88 201 204
Fragments arranged by size:
421 393 204 201 88 67 27
BanI G'GyrC_C
Cuts at: 0 300 512 709 1401
Size: 300 212 197 692
Fragments arranged by size:
692 300 212 197
BanII G_rGCy'C
Cuts at: 0 178 214 380 416 467 707 868 1204
Size: 178 36 166 36 51 240 161 336
Cuts at: 1204 1401
Size: 197
Fragments arranged by size:
336 240 197 178 166 161 51 36
36
BbeI G_GCGC'C
Cuts at: 0 713 1401
Size: 713 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 420 454 747 781 1188 1222 1401
Size: 420 34 293 34 407 34 179
Fragments arranged by size:
420 407 293 179 34 34 34
BfrBI ATG'CAT
Cuts at: 0 825 1137 1401
Size: 825 312 264
Fragments arranged by size:
825 312 264
BglI GCCn_nnn'nGGC
Cuts at: 0 427 528 1401
Size: 427 101 873
Fragments arranged by size:
873 427 101
BglII A'GATC_T
Cuts at: 0 1010 1401
Size: 1010 391
BlpI GC'TnA_GC
Cuts at: 0 172 776 781 1401
Size: 172 604 5 620
Fragments arranged by size:
620 604 172 5
Bme1580I G_kGCm'C
Cuts at: 0 305 515 533 707 868 907 965 1401
Size: 305 210 18 174 161 39 58 436
Fragments arranged by size:
436 305 210 174 161 58 39 18
BmgBI CAC'GTC
Cuts at: 0 885 1401
Size: 885 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 595 903 1401
Size: 595 308 498
Fragments arranged by size:
595 498 308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 1401
Size: 408 993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1401
Size: 431 970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1220 1401
Size: 1018 202 181
Fragments arranged by size:
1018 202 181
BsaAI yAC'GTr
Cuts at: 0 534 1401
Size: 534 867
BsaHI Gr'CG_yC
Cuts at: 0 710 1401
Size: 710 691
BsaWI w'CCGG_w
Cuts at: 0 806 1401
Size: 806 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 671 701 739 769 904 934 1186 1216
Size: 671 30 38 30 135 30 252 30
Cuts at: 1216 1401
Size: 185
Fragments arranged by size:
671 252 185 135 38 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 1401
Size: 913 488
BseYI C'CCAG_C
Cuts at: 0 39 304 508 1401
Size: 39 265 204 893
Fragments arranged by size:
893 265 204 39
BsiEI CG_ry'CG
Cuts at: 0 582 635 807 1122 1401
Size: 582 53 172 315 279
Fragments arranged by size:
582 315 279 172 53
BsiHKAI G_wGCw'C
Cuts at: 0 214 965 1204 1401
Size: 214 751 239 197
Fragments arranged by size:
751 239 214 197
Bsp1286I G_dGCh'C
Cuts at: 0 178 214 305 380 416 467 515 533
Size: 178 36 91 75 36 51 48 18
Cuts at: 533 707 868 907 965 1204 1401
Size: 174 161 39 58 239 197
Fragments arranged by size:
239 197 178 174 161 91 75 58
51 48 39 36 36 18
BspHI T'CATG_A
Cuts at: 0 1227 1401
Size: 1227 174
BsrFI r'CCGG_y
Cuts at: 0 93 421 634 785 1401
Size: 93 328 213 151 616
Fragments arranged by size:
616 328 213 151 93
BssHII G'CGCG_C
Cuts at: 0 59 1401
Size: 59 1342
BssSI C'ACGA_G
Cuts at: 0 73 1401
Size: 73 1328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1175 1401
Size: 1175 226
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 1366 1401
Size: 252 1114 35
Fragments arranged by size:
1114 252 35
BstYI r'GATC_y
Cuts at: 0 1010 1037 1401
Size: 1010 27 364
Fragments arranged by size:
1010 364 27
BtgI C'CryG_G
Cuts at: 0 182 699 848 1401
Size: 182 517 149 553
Fragments arranged by size:
553 517 182 149
EaeI y'GGCC_r
Cuts at: 0 579 605 632 1401
Size: 579 26 27 769
Fragments arranged by size:
769 579 27 26
EagI C'GGCC_G
Cuts at: 0 579 632 1401
Size: 579 53 769
Fragments arranged by size:
769 579 53
EarI CTCTTCn'nnn_
Cuts at: 0 421 790 1401
Size: 421 369 611
Fragments arranged by size:
611 421 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 1401
Size: 349 1052
EcoICRI GAG'CTC
Cuts at: 0 212 1202 1401
Size: 212 990 199
Fragments arranged by size:
990 212 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 431 798 1401
Size: 185 246 367 603
Fragments arranged by size:
603 367 246 185
EcoO109I rG'GnC_Cy
Cuts at: 0 864 1049 1401
Size: 864 185 352
Fragments arranged by size:
864 352 185
FspI TGC'GCA
Cuts at: 0 1210 1401
Size: 1210 191
HaeII r_GCGC'y
Cuts at: 0 320 509 641 713 1401
Size: 320 189 132 72 688
Fragments arranged by size:
688 320 189 132 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1401
Size: 904 32 465
Fragments arranged by size:
904 465 32
HincII GTy'rAC
Cuts at: 0 948 1401
Size: 948 453
KasI G'GCGC_C
Cuts at: 0 709 1401
Size: 709 692
MscI TGG'CCA
Cuts at: 0 607 1401
Size: 607 794
MslI CAynn'nnrTG
Cuts at: 0 667 673 679 958 1058 1136 1138 1401
Size: 667 6 6 279 100 78 2 263
Fragments arranged by size:
667 279 263 100 78 6 6 2
MspA1I CmG'CkG
Cuts at: 0 160 184 451 780 1401
Size: 160 24 267 329 621
Fragments arranged by size:
621 329 267 160 24
NaeI GCC'GGC
Cuts at: 0 95 423 636 787 1401
Size: 95 328 213 151 614
Fragments arranged by size:
614 328 213 151 95
NarI GG'CG_CC
Cuts at: 0 710 1401
Size: 710 691
NcoI C'CATG_G
Cuts at: 0 848 1401
Size: 848 553
NgoMIV G'CCGG_C
Cuts at: 0 93 421 634 785 1401
Size: 93 328 213 151 616
Fragments arranged by size:
616 328 213 151 93
NsiI A_TGCA'T
Cuts at: 0 827 1139 1401
Size: 827 312 262
Fragments arranged by size:
827 312 262
NspI r_CATG'y
Cuts at: 0 445 844 859 1137 1141 1401
Size: 445 399 15 278 4 260
Fragments arranged by size:
445 399 278 260 15 4
PasI CC'CwG_GG
Cuts at: 0 34 252 1401
Size: 34 218 1149
Fragments arranged by size:
1149 218 34
PciI A'CATG_T
Cuts at: 0 840 1401
Size: 840 561
PfoI T'CCnGG_A
Cuts at: 0 11 1401
Size: 11 1390
PmlI CAC'GTG
Cuts at: 0 534 1401
Size: 534 867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 1401
Size: 340 32 1029
Fragments arranged by size:
1029 340 32
PpuMI rG'GwC_Cy
Cuts at: 0 1049 1401
Size: 1049 352
PshAI GACnn'nnGTC
Cuts at: 0 841 1401
Size: 841 560
PspOMI G'GGCC_C
Cuts at: 0 703 864 1401
Size: 703 161 537
Fragments arranged by size:
703 537 161
PstI C_TGCA'G
Cuts at: 0 1341 1401
Size: 1341 60
PvuII CAG'CTG
Cuts at: 0 451 780 1401
Size: 451 329 621
Fragments arranged by size:
621 451 329
SacI G_AGCT'C
Cuts at: 0 214 1204 1401
Size: 214 990 197
Fragments arranged by size:
990 214 197
SacII CC_GC'GG
Cuts at: 0 185 1401
Size: 185 1216
SapI GCTCTTCn'nnn_
Cuts at: 0 421 790 1401
Size: 421 369 611
Fragments arranged by size:
611 421 369
SexAI A'CCwGG_T
Cuts at: 0 545 1401
Size: 545 856
SfcI C'TryA_G
Cuts at: 0 654 1295 1337 1401
Size: 654 641 42 64
Fragments arranged by size:
654 641 64 42
SfoI GGC'GCC
Cuts at: 0 711 1401
Size: 711 690
SmaI CCC'GGG
Cuts at: 0 489 910 998 1401
Size: 489 421 88 403
Fragments arranged by size:
489 421 403 88
SmlI C'TyrA_G
Cuts at: 0 1033 1235 1401
Size: 1033 202 166
Fragments arranged by size:
1033 202 166
SphI G_CATG'C
Cuts at: 0 445 1401
Size: 445 956
StyI C'CwwG_G
Cuts at: 0 848 1401
Size: 848 553
TatI w'GTAC_w
Cuts at: 0 935 1401
Size: 935 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 739 771 1186 1218 1401
Size: 671 32 36 32 415 32 183
Fragments arranged by size:
671 415 183 36 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 828 855 1401
Size: 531 297 27 546
Fragments arranged by size:
546 531 297 27
XmaI C'CCGG_G
Cuts at: 0 487 908 996 1401
Size: 487 421 88 405
Fragments arranged by size:
487 421 405 88
XmnI GAAnn'nnTTC
Cuts at: 0 115 979 1069 1401
Size: 115 864 90 332
Fragments arranged by size:
864 332 115 90
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AscI
AvaI BanI BanII BbeI BcgI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SapI SexAI SfcI SfoI SmaI SmlI SphI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BmtI Bpu10I BsaI BsaBI
BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI PacI PflMI PmeI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI ZraI